Strain identifier

BacDive ID: 6247

Type strain: Yes

Species: Intrasporangium mesophilum

Strain history: DSM 23217 <-- S.-K. Tang YIM 49065 <-- L.-L. Yang.

NCBI tax ID(s): 889944 (species)

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General

@ref: 16753

BacDive-ID: 6247

DSM-Number: 23217

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Intrasporangium mesophilum DSM 23217 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from rhizospheric soil of Jatropha curcas.

NCBI tax id

  • NCBI tax id: 889944
  • Matching level: species

strain history

@refhistory
16753<- S.-K. Tang, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China <- L.-L. Yang
67770DSM 23217 <-- S.-K. Tang YIM 49065 <-- L.-L. Yang.

doi: 10.13145/bacdive6247.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Intrasporangiaceae
  • genus: Intrasporangium
  • species: Intrasporangium mesophilum
  • full scientific name: Intrasporangium mesophilum Yang et al. 2012

@ref: 16753

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Intrasporangiaceae

genus: Intrasporangium

species: Intrasporangium mesophilum

full scientific name: Intrasporangium mesophilum Yang et al. 2012

type strain: yes

Morphology

cell morphology

  • @ref: 30144
  • gram stain: positive
  • motility: no

colony morphology

  • @ref: 16753
  • incubation period: 3-7 days

pigmentation

  • @ref: 30144
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16753GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
16753TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16753positivegrowth28mesophilic
30144positivegrowth20-37
30144positiveoptimum28mesophilic
67770positivegrowth28mesophilic

culture pH

  • @ref: 30144
  • ability: positive
  • type: growth
  • pH: 06-08

Physiology and metabolism

oxygen tolerance

  • @ref: 30144
  • oxygen tolerance: aerobe

halophily

  • @ref: 30144
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3014428757fructose+carbon source
3014417234glucose+carbon source
3014433942ribose+carbon source
3014417992sucrose+carbon source
3014418222xylose+carbon source
3014417632nitrate+reduction

enzymes

@refvalueactivityec
30144catalase+1.11.1.6
30144gelatinase+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
16753rhizospheric soil of Jatropha curcasJatropha curcasYunnan Province, ChuxiongChinaCHNAsia
67770Rhizospheric soil of an oilseed plant, Jatropha curcasJatropha curcasChuxiong, Yunnan ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 16753
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16753
  • description: Intrasporangium mesophilum strain YIM 49065 16S ribosomal RNA gene, partial sequence
  • accession: HQ204221
  • length: 1417
  • database: ena
  • NCBI tax ID: 889944

GC content

@refGC-contentmethod
1675369.6high performance liquid chromatography (HPLC)
3014469.6

External links

@ref: 16753

culture collection no.: DSM 23217, CCTCC AA 209077, JCM 19548, YIM 49065

straininfo link

  • @ref: 75725
  • straininfo: 408106

literature

  • topic: Phylogeny
  • Pubmed-ID: 21685254
  • title: Proposal of Intrasporangium mesophilum sp. nov., and reclassification of Humihabitans oryzae Kageyama et al. 2007 as Intrasporangium oryzae comb. nov.
  • authors: Yang LL, Ao T, Wang XH, He J, Klenk HP, Tang SK, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.029934-0
  • year: 2011
  • mesh: Actinomycetales/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Jatropha/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16753Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23217)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23217
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30144Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2649528776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
75725Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID408106.1StrainInfo: A central database for resolving microbial strain identifiers