Strain identifier

BacDive ID: 6183

Type strain: Yes

Species: Hyphomicrobium methylovorum

Strain Designation: KM 146

Strain history: T. Urakami TK 0412 <-- IFO 14180 <-- Y. Izumi KM146.

NCBI tax ID(s): 84 (species)

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General

@ref: 2173

BacDive-ID: 6183

DSM-Number: 5458

keywords: 16S sequence, Bacteria, mesophilic

description: Hyphomicrobium methylovorum KM 146 is a mesophilic bacterium that was isolated from soil samples.

NCBI tax id

  • NCBI tax id: 84
  • Matching level: species

strain history

@refhistory
2173<- IFO <- Dept. Agric. Chem., Kyoto Univ.
67770T. Urakami TK 0412 <-- IFO 14180 <-- Y. Izumi KM146.

doi: 10.13145/bacdive6183.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Hyphomicrobiaceae
  • genus: Hyphomicrobium
  • species: Hyphomicrobium methylovorum
  • full scientific name: Hyphomicrobium methylovorum Izumi et al. 1983

@ref: 2173

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Hyphomicrobiaceae

genus: Hyphomicrobium

species: Hyphomicrobium methylovorum

full scientific name: Hyphomicrobium methylovorum Izumi et al. 1983

strain designation: KM 146

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 2173
  • name: HYPHOMICROBIUM METHYLOVORUM MEDIUM (DSMZ Medium 619)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/619
  • composition: Name: HYPHOMICROBIUM METHYLOVORUM MEDIUM (DSMZ Medium 619) Composition: Agar 15.0 g/l Methanol 7.92 g/l (NH4)2HPO4 3.0 g/l NaCl 1.0 g/l MgSO4 x 7 H2O 0.2 g/l FeSO4 x 7 H2O 0.01 g/l MnSO4 x 1 H2O 0.005 g/l Inositol 0.001 g/l Choline 0.0005 g/l Riboflavin 0.0002 g/l Niacin 0.0002 g/l Pyridoxine hydrochloride 0.0002 g/l Calcium pantothenate 0.0002 g/l p-Aminobenzoic acid 0.0001 g/l Thiamine-HCl x 2 H2O 0.0001 g/l Biotin 1e-06 g/l Folic acid 1e-06 g/l Vitamin B12 1e-08 g/l Tap water Distilled water

culture temp

@refgrowthtypetemperaturerange
2173positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: Q-9

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
2173soil samplesJapanJPNAsia
67770Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 2173
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Hyphomicrobium methylovorum partial 16S rRNA gene, strain DSM 5458Y143071420ena84
20218Hyphomicrobium methylovorum gene for 16S rRNA, partial sequence, strain: NBRC 14180AB6805791406ena84

GC content

@refGC-contentmethod
6777060.2thermal denaturation, midpoint method (Tm)
6777060.6thermal denaturation, midpoint method (Tm)

External links

@ref: 2173

culture collection no.: DSM 5458, ATCC 35216, IFO 14180, NBRC 14180, JCM 6890, BCRC 15013

straininfo link

  • @ref: 75665
  • straininfo: 126030

literature

  • topic: Enzymology
  • Pubmed-ID: 8452223
  • title: Enzymatic assay for L-serine and glyoxylate involving the enzymes in the serine pathway of a methylotroph.
  • authors: Yoshida T, Mitsunaga T, Yamada H, Izumi Y
  • journal: Anal Biochem
  • DOI: 10.1006/abio.1993.1049
  • year: 1993
  • mesh: Alcohol Oxidoreductases/isolation & purification, Bacteria/enzymology, Biochemistry/methods, Evaluation Studies as Topic, Glyoxylates/*analysis/isolation & purification, Hydroxypyruvate Reductase, Serine/*analysis/isolation & purification, Transaminases/isolation & purification
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2173Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5458)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5458
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
75665Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID126030.1StrainInfo: A central database for resolving microbial strain identifiers