Strain identifier
BacDive ID: 6183
Type strain: 
Species: Hyphomicrobium methylovorum
Strain Designation: KM 146
Strain history: T. Urakami TK 0412 <-- IFO 14180 <-- Y. Izumi KM146.
NCBI tax ID(s): 84 (species)
version 9 (current version)
General
@ref: 2173
BacDive-ID: 6183
DSM-Number: 5458
keywords: 16S sequence, Bacteria
description: Hyphomicrobium methylovorum KM 146 is a bacterium that was isolated from soil samples.
NCBI tax id
- NCBI tax id: 84
- Matching level: species
strain history
| @ref | history |
|---|---|
| 2173 | <- IFO <- Dept. Agric. Chem., Kyoto Univ. |
| 67770 | T. Urakami TK 0412 <-- IFO 14180 <-- Y. Izumi KM146. |
doi: 10.13145/bacdive6183.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Hyphomicrobiaceae
- genus: Hyphomicrobium
- species: Hyphomicrobium methylovorum
- full scientific name: Hyphomicrobium methylovorum Izumi et al. 1983
@ref: 2173
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Hyphomicrobiaceae
genus: Hyphomicrobium
species: Hyphomicrobium methylovorum
full scientific name: Hyphomicrobium methylovorum Izumi et al. 1983
strain designation: KM 146
type strain: yes
Culture and growth conditions
culture medium
- @ref: 2173
- name: HYPHOMICROBIUM METHYLOVORUM MEDIUM (DSMZ Medium 619)
- growth: yes
- link: https://mediadive.dsmz.de/medium/619
- composition: Name: HYPHOMICROBIUM METHYLOVORUM MEDIUM (DSMZ Medium 619) Composition: Agar 15.0 g/l Methanol 7.92 g/l (NH4)2HPO4 3.0 g/l NaCl 1.0 g/l MgSO4 x 7 H2O 0.2 g/l FeSO4 x 7 H2O 0.01 g/l MnSO4 x 1 H2O 0.005 g/l Inositol 0.001 g/l Choline 0.0005 g/l Riboflavin 0.0002 g/l Niacin 0.0002 g/l Pyridoxine hydrochloride 0.0002 g/l Calcium pantothenate 0.0002 g/l p-Aminobenzoic acid 0.0001 g/l Thiamine-HCl x 2 H2O 0.0001 g/l Biotin 1e-06 g/l Folic acid 1e-06 g/l Vitamin B12 1e-08 g/l Tap water Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 2173 | positive | growth | 30 |
| 67770 | positive | growth | 30 |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: Q-9
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 2173 | soil samples | Japan | JPN | Asia |
| 67770 | Soil |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
- @ref: 2173
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Hyphomicrobium methylovorum partial 16S rRNA gene, strain DSM 5458 | Y14307 | 1420 | nuccore | 84 |
| 20218 | Hyphomicrobium methylovorum gene for 16S rRNA, partial sequence, strain: NBRC 14180 | AB680579 | 1406 | nuccore | 84 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 67770 | 60.2 | thermal denaturation, midpoint method (Tm) |
| 67770 | 60.6 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 2173
culture collection no.: DSM 5458, ATCC 35216, IFO 14180, NBRC 14180, JCM 6890, BCRC 15013
straininfo link
- @ref: 75665
- straininfo: 126030
literature
- topic: Enzymology
- Pubmed-ID: 8452223
- title: Enzymatic assay for L-serine and glyoxylate involving the enzymes in the serine pathway of a methylotroph.
- authors: Yoshida T, Mitsunaga T, Yamada H, Izumi Y
- journal: Anal Biochem
- DOI: 10.1006/abio.1993.1049
- year: 1993
- mesh: Alcohol Oxidoreductases/isolation & purification, Bacteria/enzymology, Biochemistry/methods, Evaluation Studies as Topic, Glyoxylates/*analysis/isolation & purification, Hydroxypyruvate Reductase, Serine/*analysis/isolation & purification, Transaminases/isolation & purification
- topic2: Phylogeny
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 2173 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5458) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-5458 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 75665 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID126030.1 | StrainInfo: A central database for resolving microbial strain identifiers |