Archive BacDiveID:10.13145/bacdive6149.20230509.8.1

Strain identifier

BacDive ID: 6149

Type strain:

Species: Acidihalobacter aeolianus

Strain Designation: V6

Strain history: E. Watkin; Curtin Univ., Australia <-- DSM 14174 <-- P. R. Norris; Univ. Warwick, UK; V6.

NCBI tax ID(s): 2792603 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 5241

BacDive-ID: 6149

DSM-Number: 14174

keywords: genome sequence, 16S sequence, Bacteria, aerobe, halotolerant, chemolithoautotroph, mesophilic, Gram-negative, motile, rod-shaped

description: Acidihalobacter aeolianus V6 is an aerobe, halotolerant, chemolithoautotroph bacterium that was isolated from acidic, salty water.

NCBI tax id

NCBI tax id: 2792603
Matching level: species

strain history

@ref	history
5241	<- P. R. Norris, Univ. Warwick, Coventry, U.K.; V6 (Acidihalobacter prosperus) <- P. R. Norris {1998}
67770	E. Watkin; Curtin Univ., Australia <-- DSM 14174 <-- P. R. Norris; Univ. Warwick, UK; V6.

doi: 10.13145/bacdive6149.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Chromatiales
family: Ectothiorhodospiraceae
genus: Acidihalobacter
species: Acidihalobacter aeolianus
full scientific name: Acidihalobacter aeolianus Khaleque et al. 2019

@ref: 5241

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Hydrogenophilales

family: Ectothiorhodospiraceae

genus: Acidihalobacter

species: Acidihalobacter aeolianus

full scientific name: Acidihalobacter aeolianus Khaleque et al. 2019

strain designation: V6

type strain: yes

Morphology

cell morphology

@ref	gram stain	cell length	cell shape	motility	confidence
67866	negative	1-2 µm	rod-shaped	yes
69480				yes	94.683
69480	negative				99.999

colony morphology

@ref: 5241
incubation period: 2-3 days

Culture and growth conditions

culture medium

@ref: 5241
name: ACIDIHALOBACTER PROSPERUS MEDIUM (DSMZ Medium 477)
growth: yes
link: https://mediadive.dsmz.de/medium/477
composition: Name: ACIDIHALOBACTER PROSPERUS MEDIUM (DSMZ Medium 477; with strain-specific modifications) Composition: FeSO4 x 7 H2O 19.9601 g/l MgCl2 x 6 H2O 2.74451 g/l NH4Cl 1.2475 g/l KCl 0.329341 g/l K2S4O6 0.299401 g/l KH2PO4 0.139721 g/l K2HPO4 0.139721 g/l MgSO4 x 7 H2O 0.0299401 g/l Nitrilotriacetic acid 0.0149701 g/l NaCl 0.00998004 g/l MnSO4 x H2O 0.00499002 g/l NiCl2 x 6 H2O 0.00199601 g/l CoSO4 x 7 H2O 0.00179641 g/l ZnSO4 x 7 H2O 0.00179641 g/l CaCl2 x 2 H2O 0.000998004 g/l AlK(SO4)2 x 12 H2O 0.000199601 g/l H3BO3 9.98004e-05 g/l Na2MoO4 x 2 H2O 9.98004e-05 g/l CuSO4 x 5 H2O 9.98004e-05 g/l Na2WO4 x 2 H2O 3.99202e-06 g/l Na2SeO3 x 5 H2O 2.99401e-06 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
5241	positive	growth	35	mesophilic
67770	positive	growth	35	mesophilic
67866	positive	growth	26-42
67866	positive	optimum	36	mesophilic

culture pH

@ref	ability	type	pH	PH range
67866	positive	growth	1.5-3	acidophile
67866	positive	optimum	1.8

Physiology and metabolism

oxygen tolerance

@ref: 67866
oxygen tolerance: aerobe

nutrition type

@ref: 67866
type: chemolithoautotroph

spore formation

@ref	spore formation	confidence
69481	no	100
69480	no	99.998

halophily

@ref	halophily level	salt	growth	tested relation	concentration
67866	halotolerant	NaCl	positive	growth	0.06-1-283 M
67866		NaCl	positive	optimum	0.428 M

observation

@ref: 67866
observation: Grows optimally on the sulfide mineral pyrite at 256 mM NaCl.

metabolite utilization

@ref: 67866
Chebi-ID: 15226
metabolite: tetrathionate
utilization activity: +
kind of utilization tested: electron donor

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
5241	acidic, salty water	Aeolian Islands, Vulcano	Italy	ITA	Europe
67770	Acidic, salty water, Vulcano	Aeolian Islands	Italy	ITA	Europe
67866	shallow pools of acidic, salty water in Vulcano	Aeolian Islands, Vulcano	Italy	ITA	Europe

isolation source categories

Cat1	Cat2
#Environmental	#Aquatic
#Condition	#Acidic
#Condition	#Saline

taxonmaps

@ref: 69479
File name: preview.99_5314.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_56;96_1688;97_2021;98_3960;99_5314&stattab=map
Last taxonomy: Acidihalobacter aeolianus
16S sequence: EU653290
Sequence Identity:
Total samples: 59
soil counts: 10
aquatic counts: 39
animal counts: 6
plant counts: 4

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
5241	Thiobacillus prosperus strain V6 16S ribosomal RNA gene, partial sequence	EU653290	1460	ena	160660
67770	Acidihalobacter prosperus strain V6 sequence	CP017448	3363634	ena	2792603

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Acidihalobacter aeolianus V6	GCA_001753165	chromosome	ncbi	2792603
66792	Acidihalobacter prosperus strain V6	160660.18	plasmid	patric	160660
66792	Acidihalobacter prosperus strain V6	160660.9	complete	patric	160660

GC content

@ref: 67866
GC-content: 62.2
method: genome sequence analysis

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	100	no
motile	yes	90.928	yes
gram-positive	no	98.836	no
anaerobic	no	96.807	yes
aerobic	no	53.845	yes
halophile	no	87.498	no
spore-forming	no	93.441	no
thermophile	no	96.905	no
glucose-util	yes	66.461	no
flagellated	yes	57.369	no
glucose-ferment	no	87.915	no

External links

@ref: 5241

culture collection no.: DSM 14174, JCM 32253

straininfo link

@ref: 75636
straininfo: 398162

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	30835194	Genome-based classification of two halotolerant extreme acidophiles, Acidihalobacter prosperus V6 (=DSM 14174 =JCM 32253) and 'Acidihalobacter ferrooxidans' V8 (=DSM 14175 =JCM 32254) as two new species, Acidihalobacter aeolianus sp. nov. and Acidihalobacter ferrooxydans sp. nov., respectively.	Khaleque HN, Gonzalez C, Kaksonen AH, Boxall NJ, Holmes DS, Watkin ELJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003313	2019	Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/classification/isolation & purification, Genome, Bacterial, Italy, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA	Genetics
	35516430	Prediction and Inferred Evolution of Acid Tolerance Genes in the Biotechnologically Important Acidihalobacter Genus.	Boase K, Gonzalez C, Vergara E, Neira G, Holmes D, Watkin E	Front Microbiol	10.3389/fmicb.2022.848410	2022

Reference

@id	authors	catalogue	doi/url	title	journal
5241	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14174)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-14174
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
67866	Himel N. Khaleque, Carolina González, Anna H. Kaksonen, Naomi J. Boxall, David S. Holmes, Elizabeth L. J. Watkin		10.1099/ijsem.0.003313	Genome-based classification of two halotolerant extreme acidophiles, Acidihalobacter prosperus V6 (=DSM 14174 =JCM 32253) and 'Acidihalobacter ferrooxidans' V8 (=DSM 14175 =JCM 32254) as two new species, Acidihalobacter aeolianus sp. nov. and Acidihalobacter ferrooxydans sp. nov., respectively	IJSEM 69: 1557-1565 2019
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
75636	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID398162.1	StrainInfo: A central database for resolving microbial strain identifiers