Strain identifier

BacDive ID: 6149

Type strain: Yes

Species: Acidihalobacter aeolianus

Strain Designation: V6

Strain history: E. Watkin; Curtin Univ., Australia <-- DSM 14174 <-- P. R. Norris; Univ. Warwick, UK; V6.

NCBI tax ID(s): 2792603 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5241

BacDive-ID: 6149

DSM-Number: 14174

keywords: rod-shaped, Gram-negative, motile, mesophilic, chemolithoautotroph, halotolerant, aerobe, Bacteria, 16S sequence, genome sequence

description: Acidihalobacter aeolianus V6 is an aerobe, halotolerant, chemolithoautotroph Proteobacterium that was isolated from acidic, salty water.

NCBI tax id

  • NCBI tax id: 2792603
  • Matching level: species

strain history

doi: 10.13145/bacdive6149.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Ectothiorhodospiraceae
  • genus: Acidihalobacter
  • species: Acidihalobacter aeolianus
  • full scientific name: Acidihalobacter aeolianus Khaleque et al. 2019

@ref: 5241

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Hydrogenophilales

family: Ectothiorhodospiraceae

genus: Acidihalobacter

species: Acidihalobacter aeolianus

full scientific name: Acidihalobacter aeolianus Khaleque et al. 2019

strain designation: V6

type strain: yes

Morphology

cell morphology

  • @ref: 67866
  • gram stain: negative
  • cell length: 1-2 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 5241
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 5241
  • name: ACIDIHALOBACTER PROSPERUS MEDIUM (DSMZ Medium 477)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/477
  • composition: Name: ACIDIHALOBACTER PROSPERUS MEDIUM (DSMZ Medium 477; with strain-specific modifications) Composition: FeSO4 x 7 H2O 20.0 g/l MgCl2 x 6 H2O 2.75 g/l NH4Cl 1.25 g/l KCl 0.33 g/l K2S4O6 0.3 g/l K2HPO4 0.14 g/l KH2PO4 0.14 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l NiCl2 x 6 H2O 0.002 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
5241positivegrowth35mesophilic
67770positivegrowth35mesophilic
67866positivegrowth26-42
67866positiveoptimum36mesophilic

culture pH

@refabilitytypepH
67866positivegrowth1.5-3
67866positiveoptimum1.8

Physiology and metabolism

oxygen tolerance

  • @ref: 67866
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 67866
  • type: chemolithoautotroph

halophily

@refhalophily levelsaltgrowthtested relationconcentration
67866halotolerantNaClpositivegrowth0.06-1-283 M
67866NaClpositiveoptimum0.428 M

observation

  • @ref: 67866
  • observation: Grows optimally on the sulfide mineral pyrite at 256 mM NaCl.

metabolite utilization

  • @ref: 67866
  • Chebi-ID: 15226
  • metabolite: tetrathionate
  • utilization activity: +
  • kind of utilization tested: electron donor

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5241acidic, salty waterAeolian Islands, VulcanoItalyITAEurope
67770Acidic, salty water, VulcanoAeolian IslandsItalyITAEurope
67866shallow pools of acidic, salty water in VulcanoAeolian Islands, VulcanoItalyITAEurope

isolation source categories

Cat1Cat2
#Environmental#Aquatic
#Condition#Acidic
#Condition#Saline

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5241Thiobacillus prosperus strain V6 16S ribosomal RNA gene, partial sequenceEU6532901460ena160660
67770Acidihalobacter prosperus strain V6 sequenceCP0174483363634ena2792603

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acidihalobacter aeolianus V6GCA_001753165chromosomencbi2792603
66792Acidihalobacter prosperus strain V6160660.18plasmidpatric160660
66792Acidihalobacter prosperus strain V6160660.9completepatric160660
67770Acidihalobacter prosperus strain V6 sequenceCP017448ena2792603

GC content

  • @ref: 67866
  • GC-content: 62.2
  • method: genome sequence analysis

External links

@ref: 5241

culture collection no.: DSM 14174, JCM 32253

Reference

@idauthorscataloguedoi/urltitlejournal
5241Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14174)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14174
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67866Himel N. Khaleque, Carolina González, Anna H. Kaksonen, Naomi J. Boxall, David S. Holmes, Elizabeth L. J. Watkin10.1099/ijsem.0.003313Genome-based classification of two halotolerant extreme acidophiles, Acidihalobacter prosperus V6 (=DSM 14174 =JCM 32253) and 'Acidihalobacter ferrooxidans' V8 (=DSM 14175 =JCM 32254) as two new species, Acidihalobacter aeolianus sp. nov. and Acidihalobacter ferrooxydans sp. nov., respectivelyIJSEM 69: 1557-1565 2019