Strain identifier
BacDive ID: 6140
Type strain: 
Species: Annwoodia aquaesulis
Strain history: <- A. P. Wood, University of Warwick, Department of Environmental Sciences, Coventry, UK <- A. P. Wood {1984}
NCBI tax ID(s): 47959 (species)
version 8.1 (current version)
General
@ref: 1635
BacDive-ID: 6140
DSM-Number: 4255
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, chemolithoautotroph, thermophilic, Gram-negative, motile, rod-shaped
description: Annwoodia aquaesulis DSM 4255 is an anaerobe, chemolithoautotroph, thermophilic bacterium that was isolated from thermal sulfur spring.
NCBI tax id
- NCBI tax id: 47959
- Matching level: species
strain history
- @ref: 1635
- history: <- A. P. Wood, University of Warwick, Department of Environmental Sciences, Coventry, UK <- A. P. Wood {1984}
doi: 10.13145/bacdive6140.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Nitrosomonadales
- family: Thiobacillaceae
- genus: Annwoodia
- species: Annwoodia aquaesulis
- full scientific name: Annwoodia aquaesulis (Wood and Kelly 1995) Boden et al. 2017
synonyms
- @ref: 20215
- synonym: Thiobacillus aquaesulis
@ref: 1635
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Hydrogenophilales
family: Thiobacillaceae
genus: Annwoodia
species: Annwoodia aquaesulis
full scientific name: Annwoodia aquaesulis (Wood and Kelly 1995) Boden et al. 2017
type strain: yes
Morphology
cell morphology
- @ref: 43206
- gram stain: negative
- cell length: 0.9 µm
- cell width: 0.3 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: monotrichous, polar
colony morphology
| @ref | incubation period | colony size | colony color | colony shape | medium used |
|---|---|---|---|---|---|
| 1635 | 3-7 days | ||||
| 43206 | 1-2 mm | white to yellow | circular | Thiosulfate agar |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 1635 | ANNWOODIA AQUAESULIS MEDIUM (DSMZ Medium 389) | yes | https://mediadive.dsmz.de/medium/389 | Name: ANNWOODIA MEDIUM (DSMZ Medium 389) Composition: Agar 15.0 g/l Na2HPO4 x 2 H2O 7.9 g/l Na2S2O3 x 5 H2O 5.0 g/l KH2PO4 1.5 g/l Na2-EDTA 0.5 g/l NH4Cl 0.4 g/l ZnSO4 x 7 H2O 0.11 g/l NaOH 0.11 g/l MgSO4 x 7 H2O 0.1 g/l CaCl2 x 2 H2O 0.0734 g/l FeSO4 x 7 H2O 0.05 g/l MnCl2 x 4 H2O 0.025 g/l (NH4)6Mo7O24 x 4 H2O 0.005 g/l CoCl2 x 6 H2O 0.005 g/l CuSO4 x 5 H2O 0.002 g/l Distilled water |
| 43206 | Thiosulfate agar | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 1635 | positive | growth | 42 | thermophilic |
| 43206 | positive | optimum | 45 | thermophilic |
| 43206 | positive | growth | 30-55 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 43206 | positive | growth | 7.0-9.0 | alkaliphile |
| 43206 | positive | optimum | 7.6 |
Physiology and metabolism
oxygen tolerance
- @ref: 43206
- oxygen tolerance: anaerobe
nutrition type
| @ref | type |
|---|---|
| 43206 | chemolithoautotroph |
| 43206 | heterotroph |
| 43206 | mixotroph |
spore formation
| @ref | type of spore | spore formation |
|---|---|---|
| 43206 | endospore | no |
| 43206 | exospore (microbial cyst) | no |
observation
| @ref | observation |
|---|---|
| 43206 | containing volutin (polyphosphate) granules |
| 43206 | accumulate polyhydroxybutyrate |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43206 | 16094 | thiosulfate | + | energy source |
| 43206 | 15987 | trithionate | + | energy source |
| 43206 | 15226 | tetrathionate | + | energy source |
| 43206 | 33403 | elemental sulfur | + | energy source |
| 43206 | 18022 | thiocyanate | - | energy source |
| 43206 | 18276 | dihydrogen | - | energy source |
| 43206 | 17437 | dimethyl sulfide | - | energy source |
| 43206 | 4608 | dimethyl disulfide | - | energy source |
| 43206 | 23012 | carbon disulfide | - | energy source |
| 43206 | 17632 | nitrate | - | nitrogen source |
| 43206 | 28938 | ammonium | + | nitrogen source |
| 43206 | 17632 | nitrate | + | reduction |
| 43206 | 15138 | sulfide | + | energy source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 43206 | catalase | + | 1.11.1.6 |
| 43206 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 1635 | thermal sulfur spring | Avon, Great Roman Bath at Bath | United Kingdom | GBR | Europe |
| 43206 | thermal sulfur spring | Bath, UK | United Kingdom | GBR | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Thermal spring |
| #Condition | #Sulfuric |
taxonmaps
- @ref: 69479
- File name: preview.99_83977.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_421;96_11500;97_13918;98_17160;99_83977&stattab=map
- Last taxonomy: Annwoodia aquaesulis subclade
- 16S sequence: U58019
- Sequence Identity:
- Total samples: 505
- soil counts: 68
- aquatic counts: 394
- animal counts: 36
- plant counts: 7
Safety information
risk assessment
- @ref: 1635
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Thiobacillus aquaesulis 16S ribosomal RNA gene, complete sequence | U58019 | 1496 | ena | 47959 |
| 1635 | Thiobacillus aquaesulis partial 16S rRNA gene, type strain DSM 4255T | HE971728 | 1525 | ena | 47959 |
Genome sequences
- @ref: 66792
- description: Annwoodia aquaesulis DSM 4255
- accession: 2765235834
- assembly level: draft
- database: img
- NCBI tax ID: 47959
GC content
- @ref: 1635
- GC-content: 65.7
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 1635
culture collection no.: DSM 4255, ATCC 43788
straininfo link
- @ref: 75627
- straininfo: 42040
literature
- topic: Phylogeny
- Pubmed-ID: 28581923
- title: Reclassification of Thiobacillus aquaesulis (Wood & Kelly, 1995) as Annwoodia aquaesulis gen. nov., comb. nov., transfer of Thiobacillus (Beijerinck, 1904) from the Hydrogenophilales to the Nitrosomonadales, proposal of Hydrogenophilalia class. nov. within the 'Proteobacteria', and four new families within the orders Nitrosomonadales and Rhodocyclales.
- authors: Boden R, Hutt LP, Rae AW
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001927
- year: 2017
- mesh: Bacterial Typing Techniques, Betaproteobacteria/*classification, DNA, Bacterial/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thiobacillus/*classification
- topic2: Transcriptome
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1635 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4255) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4255 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 43206 | Rich Boden, Lee P. Hutt and Alex W. Rae | 10.1099/ijsem.0.001927 | Reclassification of Thiobacillus aquaesulis (Wood & Kelly, 1995) as Annwoodia aquaesulis gen. nov., comb. nov., transfer of Thiobacillus (Beijerinck, 1904) from the Hydrogenophilales to the Nitrosomonadales, proposal of Hydrogenophilalia class. nov. within the Proteobacteria, and four new families within the orders Nitrosomonadales and Rhodocyclales | IJSEM 67: 1191-1205 2017 | 28581923 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 75627 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42040.1 | StrainInfo: A central database for resolving microbial strain identifiers |