Strain identifier

BacDive ID: 6048

Type strain: Yes

Species: Halomonas almeriensis

Strain Designation: M8

Strain history: CIP <- 2005, CECT <- F. Martinez-Checa: strain M8

NCBI tax ID(s): 308163 (species)

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General

@ref: 17369

BacDive-ID: 6048

DSM-Number: 23775

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Halomonas almeriensis M8 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from hypersaline water from a saltern.

NCBI tax id

  • NCBI tax id: 308163
  • Matching level: species

strain history

@refhistory
17369<- CECT <- F. Martínez-Checa <- M. J. Martínez-Cánovas, Univ. Granada, Campus Cartuja, Fac. Pharmacy, Spain
116261CIP <- 2005, CECT <- F. Martinez-Checa: strain M8

doi: 10.13145/bacdive6048.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halomonas
  • species: Halomonas almeriensis
  • full scientific name: Halomonas almeriensis Martínez-Checa et al. 2005

@ref: 17369

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halomonas

species: Halomonas almeriensis

full scientific name: Halomonas almeriensis Martínez-Checa et al. 2005

strain designation: M8

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31490negative2.25 µm0.75 µmrod-shapedno
116261negativerod-shapedno

pigmentation

  • @ref: 31490
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17369BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 30.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40066MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116261CIP Medium 204yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=204
116261CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
17369positivegrowth28mesophilic
31490positivegrowth15-37
31490positiveoptimum26mesophilic
40066positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
31490positivegrowth06-10alkaliphile
31490positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 31490
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
31490NaClpositivegrowth05-25 %
31490NaClpositiveoptimum7.5 %

observation

  • @ref: 31490
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3149016449alanine+carbon source
3149017822serine+carbon source
11626117632nitrate-reduction
11626116301nitrite-reduction

metabolite production

  • @ref: 116261
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31490acid phosphatase+3.1.3.2
31490catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116261oxidase-
116261alcohol dehydrogenase-1.1.1.1
116261catalase+1.11.1.6
116261lysine decarboxylase-4.1.1.18
116261ornithine decarboxylase-4.1.1.17
116261urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116261-+++-++--------+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17369hypersaline water from a salternAlmería, Cabo de GataSpainESPEurope
116261Environment, Saltern waterSpainESPEurope

isolation source categories

Cat1Cat2
#Environmental#Aquatic
#Condition#Saline

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
173691Risk group (German classification)
1162611Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17369
  • description: Halomonas almeriensis strain M8 16S ribosomal RNA gene, partial sequence
  • accession: AY858696
  • length: 1459
  • database: ena
  • NCBI tax ID: 308163

GC content

@refGC-contentmethod
1736963.5thermal denaturation, midpoint method (Tm)
3149063.5

External links

@ref: 17369

culture collection no.: DSM 23775, CECT 7050, LMG 22904, CIP 108960

straininfo link

  • @ref: 75535
  • straininfo: 366364

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16166703Halomonas almeriensis sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium from Cabo de Gata, Almeria, south-east Spain.Martinez-Checa F, Bejar V, Martinez-Canovas MJ, Llamas I, Quesada EInt J Syst Evol Microbiol10.1099/ijs.0.63676-02005Aerobiosis, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Halomonas/*classification/genetics/metabolism/physiology, Molecular Sequence Data, Phylogeny, Polysaccharides, Bacterial/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride, SpainMetabolism
Phylogeny17519342Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas.Giordano A, Vella FM, Romano I, Gambacorta AJ Lipid Res10.1194/jlr.M700152-JLR2002007Glycolipids/*chemistry, Halomonas/*chemistry/classification, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Sequence Data, Phosphatidylglycerols/*chemistry, Phospholipids/*chemistry, PhylogenyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17369Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23775)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23775
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31490Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2778828776041
40066Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6647
68382Automatically annotated from API zym
75535Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID366364.1StrainInfo: A central database for resolving microbial strain identifiers
116261Curators of the CIPCollection of Institut Pasteur (CIP 108960)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108960