Strain identifier

BacDive ID: 6035

Type strain: Yes

Species: Halomonas campisalis

Strain Designation: 4A, A4

Strain history: CIP <- 2000, M.R. Mormile, Dept. Biol. Sci., Univ. Missouri, USA: strain 4A

NCBI tax ID(s): 74661 (species)

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General

@ref: 5802

BacDive-ID: 6035

DSM-Number: 15413

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Halomonas campisalis 4A is a mesophilic, Gram-negative, motile bacterium that was isolated from Soil sample collected from a salt flat south of Alkali Lake.

NCBI tax id

  • NCBI tax id: 74661
  • Matching level: species

strain history

@refhistory
5802<- A. Aiken, Montana State Univ., Bozeman, USA; 4A <- M. R. Mormile, Univ. Missouri, USA
403252000, M.R. Mormile, Dept. Biol. Sci., Univ. Missouri, USA: strain 4A
117120CIP <- 2000, M.R. Mormile, Dept. Biol. Sci., Univ. Missouri, USA: strain 4A

doi: 10.13145/bacdive6035.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halomonas
  • species: Halomonas campisalis
  • full scientific name: Halomonas campisalis Mormile et al. 2000

  • synonyms

    • @ref: 20215
    • synonym: Billgrantia campisalis

@ref: 5802

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halomonas

species: Halomonas campisalis

full scientific name: Halomonas campisalis Mormile et al. 2000

strain designation: 4A, A4

type strain: yes

Morphology

cell morphology

  • @ref: 117120
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5802BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40325Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
117120CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5802positivegrowth30mesophilic
40325positivegrowth30mesophilic
117120positivegrowth25-41
117120nogrowth5psychrophilic
117120nogrowth10psychrophilic
117120nogrowth45thermophilic

Physiology and metabolism

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
117120esculin-hydrolysis4853
117120hippurate-hydrolysis606565
117120nitrate+reduction17632
117120nitrite-reduction16301

metabolite production

  • @ref: 117120
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
117120oxidase+
117120beta-galactosidase-3.2.1.23
117120alcohol dehydrogenase-1.1.1.1
117120gelatinase-
117120caseinase-3.4.21.50
117120catalase+1.11.1.6
117120tween esterase-
117120gamma-glutamyltransferase+2.3.2.2
117120lecithinase-
117120lipase-
117120lysine decarboxylase-4.1.1.18
117120ornithine decarboxylase-4.1.1.17
117120phenylalanine ammonia-lyase+4.3.1.24
117120protease-
117120tryptophan deaminase-
117120urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117120-----+---------+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117120-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117120---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5802Soil sample collected from a salt flat south of Alkali LakeWashington StateUSAUSANorth America
117120Environment, Soil sample collected from a salt flat south of Alkali LakeWashingtonUnited States of AmericaUSANorth America1996

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Soil
#Condition#Alkaline
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_16926.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_2666;97_10456;98_12816;99_16926&stattab=map
  • Last taxonomy: Halomonas
  • 16S sequence: AF054286
  • Sequence Identity:
  • Total samples: 17
  • soil counts: 4
  • aquatic counts: 8
  • animal counts: 5

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58021Risk group (German classification)
1171201Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5802
  • description: Halomonas campisalis 16S ribosomal RNA gene, partial sequence
  • accession: AF054286
  • length: 1400
  • database: ena
  • NCBI tax ID: 74661

Genome sequences

  • @ref: 66792
  • description: Halomonas campisalis A4
  • accession: GCA_022341425
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 74661

GC content

  • @ref: 5802
  • GC-content: 66.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedyes82.739no
gram-positiveno98.422no
anaerobicno97.578no
aerobicyes81.057no
halophileyes92.023no
spore-formingno95.922no
motileyes91.464no
glucose-fermentno77.909no
thermophileno96.301yes
glucose-utilyes90.914no

External links

@ref: 5802

culture collection no.: DSM 15413, ATCC 700597, CIP 106639

straininfo link

  • @ref: 75522
  • straininfo: 46968

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10794143Halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium.Mormile MR, Romine MF, Garcia MT, Ventosa A, Bailey TJ, Peyton BMSyst Appl Microbiol10.1016/S0723-2020(99)80008-31999Biodegradation, Environmental, DNA, Ribosomal/analysis/genetics, Halomonas/*classification/genetics/*isolation & purification/physiology, Hydrogen-Ion Concentration, Industrial Waste, Ion Exchange, Molecular Sequence Data, Nitrates/*metabolism, Nitrites/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Water MicrobiologyMetabolism
Phylogeny17473232Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil.Wang YN, Cai H, Yu SL, Wang ZY, Liu J, Wu XLInt J Syst Evol Microbiol10.1099/ijs.0.64826-02007Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Flagella/ultrastructure, Genes, rRNA, Gentian Violet, Halomonas/chemistry/*classification/*isolation & purification/physiology, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Molecular Sequence Data, Nucleic Acid Hybridization, Petroleum/microbiology, Phenazines, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, *Soil Microbiology, TemperatureGenetics

Reference

@idauthorscataloguedoi/urltitle
5802Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15413)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15413
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40325Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18792
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75522Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46968.1StrainInfo: A central database for resolving microbial strain identifiers
117120Curators of the CIPCollection of Institut Pasteur (CIP 106639)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106639