Strain identifier

BacDive ID: 6006

Type strain: Yes

Species: Halotalea alkalilenta

Strain Designation: AW-7

Strain history: CIP <- 2007, CECT <- 2006, S. Ntougias, Kalamata Inst., Kalamata, Greece: strain AW-7

NCBI tax ID(s): 1403347 (strain), 376489 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9.1:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.3 (current version)

General

@ref: 7133

BacDive-ID: 6006

DSM-Number: 17697

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Halotalea alkalilenta AW-7 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from olive mill waste .

NCBI tax id

NCBI tax idMatching level
376489species
1403347strain

strain history

@refhistory
7133<- S. Ntougias and G. Zervakis, Natl. Agricultural Research Foundation, Institute of Kalamata; AW-7 <- S. Ntougias
116240CIP <- 2007, CECT <- 2006, S. Ntougias, Kalamata Inst., Kalamata, Greece: strain AW-7

doi: 10.13145/bacdive6006.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halotalea
  • species: Halotalea alkalilenta
  • full scientific name: Halotalea alkalilenta Ntougias et al. 2007

@ref: 7133

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halotalea

species: Halotalea alkalilenta

full scientific name: Halotalea alkalilenta Ntougias et al. 2007

strain designation: AW-7

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
116240negativerod-shapedyes
125438negative98.5
125439negative99

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7133TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33319MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116240CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
7133positivegrowth25-32
33319positivegrowth30

Physiology and metabolism

oxygen tolerance

  • @ref: 116240
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 125439
  • spore formation: no
  • confidence: 98.7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11624017632nitrate-reduction
11624016301nitrite-reduction
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837117266L-sorbose-builds acid from
6837117634D-glucose+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

metabolite production

  • @ref: 116240
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116240oxidase+
116240catalase+1.11.1.6
116240urease-3.5.1.5
68382trypsin-3.4.21.4

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116240-+++-+-------+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116240---------+/-++/-+/--+/----------+--+/--+/-+/---+/------+/-+/--+/----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7133olive mill waste (alkaline alpeorujo)Crete, SitiaGreeceGRCEurope
116240Olive mill waste, alkaline alpeorujoSitia, CreteGreeceGRCEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Industrial waste

taxonmaps

  • @ref: 69479
  • File name: preview.99_6964.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_3094;97_4011;98_5116;99_6964&stattab=map
  • Last taxonomy: Halotalea alkalilenta subclade
  • 16S sequence: DQ421388
  • Sequence Identity:
  • Total samples: 2278
  • soil counts: 65
  • aquatic counts: 1264
  • animal counts: 886
  • plant counts: 63

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71331Risk group (German classification)
1162401Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7133
  • description: Halotalea alkalilenta strain AW-7 16S ribosomal RNA gene, partial sequence
  • accession: DQ421388
  • length: 1491
  • database: nuccore
  • NCBI tax ID: 376489

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halotalea alkalilenta DSM 17697GCA_000621205scaffoldncbi1403347
66792Halotalea alkalilenta DSM 176971403347.3wgspatric1403347
66792Halotalea alkalilenta DSM 176972556921652draftimg1403347

GC content

  • @ref: 7133
  • GC-content: 64.4

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno98.5no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.072yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes80.554no
125438spore-formingspore-formingAbility to form endo- or exosporesno85.26no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno96.478no
125438motile2+flagellatedAbility to perform flagellated movementyes68.218no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno98.7
125439BacteriaNetmotilityAbility to perform movementyes85.7
125439BacteriaNetgram_stainReaction to gram-stainingnegative99
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe71

External links

@ref: 7133

culture collection no.: DSM 17697, CECT 7134, CIP 109710

straininfo link

  • @ref: 75494
  • straininfo: 309541

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17766858Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996.Ntougias S, Zervakis GI, Fasseas CInt J Syst Evol Microbiol10.1099/ijs.0.65078-02007Aerobiosis/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Glucose/metabolism, Halomonadaceae/*classification/genetics/*isolation & purification/physiology, Hydrogen-Ion Concentration, Industrial Waste, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, Nucleic Acid Hybridization, Olea/*microbiology, Organic Chemicals/metabolism, Osmotic Pressure, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolismMetabolism
Genetics26380640High-quality permanent draft genome sequence of the extremely osmotolerant diphenol degrading bacterium Halotalea alkalilenta AW-7(T), and emended description of the genus Halotalea.Ntougias S, Lapidus A, Copeland A, Reddy TB, Pati A, Ivanova NN, Markowitz VM, Klenk HP, Woyke T, Fasseas C, Kyrpides NC, Zervakis GIStand Genomic Sci10.1186/s40793-015-0052-72015
Phylogeny31535159Phytohalomonas tamaricis gen. nov., sp. nov., an endophytic bacterium isolated from Tamarix ramosissima roots growing in Kumtag desert.Liu L, Wang SY, He CF, Zhang XX, Chi M, Liang LX, Li LBArch Microbiol10.1007/s00203-019-01724-x2019Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desert Climate, Fatty Acids/analysis, Halomonadaceae/chemistry/*classification/genetics, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Tamaricaceae/*microbiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7133Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17697)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17697
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33319Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7484
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75494Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309541.1StrainInfo: A central database for resolving microbial strain identifiers
116240Curators of the CIPCollection of Institut Pasteur (CIP 109710)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109710
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1