Strain identifier

BacDive ID: 6006

Type strain: Yes

Species: Halotalea alkalilenta

Strain Designation: AW-7

Strain history: CIP <- 2007, CECT <- 2006, S. Ntougias, Kalamata Inst., Kalamata, Greece: strain AW-7

NCBI tax ID(s): 1403347 (strain), 376489 (species)

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General

@ref: 7133

BacDive-ID: 6006

DSM-Number: 17697

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative

description: Halotalea alkalilenta AW-7 is an obligate aerobe, Gram-negative bacterium that was isolated from olive mill waste .

NCBI tax id

NCBI tax idMatching level
376489species
1403347strain

strain history

@refhistory
7133<- S. Ntougias and G. Zervakis, Natl. Agricultural Research Foundation, Institute of Kalamata; AW-7 <- S. Ntougias
116240CIP <- 2007, CECT <- 2006, S. Ntougias, Kalamata Inst., Kalamata, Greece: strain AW-7

doi: 10.13145/bacdive6006.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halotalea
  • species: Halotalea alkalilenta
  • full scientific name: Halotalea alkalilenta Ntougias et al. 2007

@ref: 7133

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halotalea

species: Halotalea alkalilenta

full scientific name: Halotalea alkalilenta Ntougias et al. 2007

strain designation: AW-7

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
116240negativerod-shapedyes
69480negative98.5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7133TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33319MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116240CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
7133positivegrowth25-32
33319positivegrowth30

Physiology and metabolism

oxygen tolerance

  • @ref: 116240
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-tagatose-builds acid from16443
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116240nitrate-reduction17632
116240nitrite-reduction16301

metabolite production

  • @ref: 116240
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116240oxidase+
116240catalase+1.11.1.6
116240urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116240-+++-+-------+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116240---------+/-++/-+/--+/----------+--+/--+/-+/---+/------+/-+/--+/----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7133olive mill waste (alkaline alpeorujo)Crete, SitiaGreeceGRCEurope
116240Olive mill waste, alkaline alpeorujoSitia, CreteGreeceGRCEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Industrial waste

taxonmaps

  • @ref: 69479
  • File name: preview.99_6964.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_3094;97_4011;98_5116;99_6964&stattab=map
  • Last taxonomy: Halotalea alkalilenta subclade
  • 16S sequence: DQ421388
  • Sequence Identity:
  • Total samples: 2278
  • soil counts: 65
  • aquatic counts: 1264
  • animal counts: 886
  • plant counts: 63

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71331Risk group (German classification)
1162401Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7133
  • description: Halotalea alkalilenta strain AW-7 16S ribosomal RNA gene, partial sequence
  • accession: DQ421388
  • length: 1491
  • database: nuccore
  • NCBI tax ID: 376489

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halotalea alkalilenta DSM 17697GCA_000621205scaffoldncbi1403347
66792Halotalea alkalilenta DSM 176971403347.3wgspatric1403347
66792Halotalea alkalilenta DSM 176972556921652draftimg1403347

GC content

  • @ref: 7133
  • GC-content: 64.4

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno98.5no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.072yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes80.554no
69480spore-formingspore-formingAbility to form endo- or exosporesno85.26no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.478no
69480flagellatedmotile2+Ability to perform flagellated movementyes68.218no

External links

@ref: 7133

culture collection no.: DSM 17697, CECT 7134, CIP 109710

straininfo link

  • @ref: 75494
  • straininfo: 309541

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17766858Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996.Ntougias S, Zervakis GI, Fasseas CInt J Syst Evol Microbiol10.1099/ijs.0.65078-02007Aerobiosis/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Glucose/metabolism, Halomonadaceae/*classification/genetics/*isolation & purification/physiology, Hydrogen-Ion Concentration, Industrial Waste, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, Nucleic Acid Hybridization, Olea/*microbiology, Organic Chemicals/metabolism, Osmotic Pressure, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolismMetabolism
Genetics26380640High-quality permanent draft genome sequence of the extremely osmotolerant diphenol degrading bacterium Halotalea alkalilenta AW-7(T), and emended description of the genus Halotalea.Ntougias S, Lapidus A, Copeland A, Reddy TB, Pati A, Ivanova NN, Markowitz VM, Klenk HP, Woyke T, Fasseas C, Kyrpides NC, Zervakis GIStand Genomic Sci10.1186/s40793-015-0052-72015
Phylogeny31535159Phytohalomonas tamaricis gen. nov., sp. nov., an endophytic bacterium isolated from Tamarix ramosissima roots growing in Kumtag desert.Liu L, Wang SY, He CF, Zhang XX, Chi M, Liang LX, Li LBArch Microbiol10.1007/s00203-019-01724-x2019Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Desert Climate, Fatty Acids/analysis, Halomonadaceae/chemistry/*classification/genetics, Multilocus Sequence Typing, Nucleic Acid Hybridization, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Tamaricaceae/*microbiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7133Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17697)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17697
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33319Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7484
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75494Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309541.1StrainInfo: A central database for resolving microbial strain identifiers
116240Curators of the CIPCollection of Institut Pasteur (CIP 109710)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109710