Strain identifier

BacDive ID: 5992

Type strain: Yes

Species: Sporohalobacter lortetii

Strain Designation: MD-2

Strain history: <- A. Oren <- ATCC <- A. Oren, MD-2

NCBI tax ID(s): 974 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 1289

BacDive-ID: 5992

DSM-Number: 3070

keywords: anaerobe, Bacteria, 16S sequence, mesophilic

description: Sporohalobacter lortetii MD-2 is an anaerobe, mesophilic bacterium that was isolated from sediment.

NCBI tax id

  • NCBI tax id: 974
  • Matching level: species

strain history: <- A. Oren <- ATCC <- A. Oren, MD-2

doi: 10.13145/bacdive5992.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Halanaerobiales
  • family: Halobacteroidaceae
  • genus: Sporohalobacter
  • species: Sporohalobacter lortetii
  • full scientific name: Sporohalobacter lortetii (Oren 1984) Oren et al. 1988
  • synonyms

    • @ref: 20215
    • synonym: Clostridium lortetii

@ref: 1289

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Halanaerobiales

family: Halobacteroidaceae

genus: Sporohalobacter

species: Sporohalobacter lortetii

full scientific name: Sporohalobacter lortetii (Oren 1984) Oren et al. 1988

strain designation: MD-2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1289
  • name: SPOROHALOBACTER LORTETII MEDIUM (DSMZ Medium 319)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/319
  • composition: Name: SPOROHALOBACTER LORTETII MEDIUM (DSMZ Medium 319) Composition: MgCl2 x 6 H2O 10.0 g/l L-Glutamic acid 4.0 g/l CaCl2 x 2 H2O 3.7 g/l Casamino acids 2.0 g/l Nutrient broth 2.0 g/l Yeast extract 2.0 g/l KCl 0.75 g/l L-Cysteine HCl x H2O 0.5 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l FeSO4 x 7 H2O 0.002 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l Pyridoxine hydrochloride 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l Riboflavin 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Nicotinic acid 5e-05 g/l Thiamine HCl 5e-05 g/l (±)-alpha-Lipoic acid 5e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

  • @ref: 1289
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1289
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1289
  • sample type: sediment
  • geographic location: Dead Sea
  • country: Israel
  • origin.country: ISR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Sediment

Safety information

risk assessment

  • @ref: 1289
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sporohalobacter lortetii strain DSM 3070 16S ribosomal RNA gene, partial sequenceKJ4773301494ena974
20218Sporohalobacter lortetii 16S ribosomal RNAM591221539ena974
1289Sporohalobacter lortetii partial 16S rRNA gene, type strain DSM 3070T, clone 1HE5827741527ena974
1289Sporohalobacter lortetii partial 16S rRNA gene, type strain DSM 3070T, clone 2HE5827821526ena974

GC content

  • @ref: 1289
  • GC-content: 31.5

External links

@ref: 1289

culture collection no.: DSM 3070, ATCC 35059

straininfo link

@refpassport
20218http://www.straininfo.net/strains/110132
20218http://www.straininfo.net/strains/110130

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1289Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3070)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3070
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274