Strain identifier

BacDive ID: 5992

Type strain: Yes

Species: Sporohalobacter lortetii

Strain Designation: MD-2

Strain history: <- A. Oren <- ATCC <- A. Oren, MD-2

NCBI tax ID(s): 974 (species)

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General

@ref: 1289

BacDive-ID: 5992

DSM-Number: 3070

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Sporohalobacter lortetii MD-2 is an anaerobe, mesophilic bacterium that was isolated from sediment.

NCBI tax id

  • NCBI tax id: 974
  • Matching level: species

strain history

  • @ref: 1289
  • history: <- A. Oren <- ATCC <- A. Oren, MD-2

doi: 10.13145/bacdive5992.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Halanaerobiales
  • family: Halobacteroidaceae
  • genus: Sporohalobacter
  • species: Sporohalobacter lortetii
  • full scientific name: Sporohalobacter lortetii (Oren 1984) Oren et al. 1988

  • synonyms

    • @ref: 20215
    • synonym: Clostridium lortetii

@ref: 1289

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Halanaerobiales

family: Halobacteroidaceae

genus: Sporohalobacter

species: Sporohalobacter lortetii

full scientific name: Sporohalobacter lortetii (Oren 1984) Oren et al. 1988

strain designation: MD-2

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1289
  • name: SPOROHALOBACTER LORTETII MEDIUM (DSMZ Medium 319)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/319
  • composition: Name: SPOROHALOBACTER LORTETII MEDIUM (DSMZ Medium 319) Composition: MgCl2 x 6 H2O 9.87167 g/l L-Glutamic acid 3.94867 g/l CaCl2 x 2 H2O 3.65252 g/l Yeast extract 1.97433 g/l Casamino acids 1.97433 g/l Nutrient broth 1.97433 g/l KCl 0.740375 g/l L-Cysteine HCl x H2O 0.493583 g/l MgSO4 x 7 H2O 0.029615 g/l Nitrilotriacetic acid 0.0148075 g/l NaCl 0.00987167 g/l MnSO4 x H2O 0.00493583 g/l FeSO4 x 7 H2O 0.00197433 g/l CoSO4 x 7 H2O 0.0017769 g/l ZnSO4 x 7 H2O 0.0017769 g/l Sodium resazurin 0.000493583 g/l NiCl2 x 6 H2O 0.00029615 g/l AlK(SO4)2 x 12 H2O 0.000197433 g/l Pyridoxine hydrochloride 9.87167e-05 g/l H3BO3 9.87167e-05 g/l Na2MoO4 x 2 H2O 9.87167e-05 g/l CuSO4 x 5 H2O 9.87167e-05 g/l p-Aminobenzoic acid 4.93583e-05 g/l Calcium D-(+)-pantothenate 4.93583e-05 g/l Nicotinic acid 4.93583e-05 g/l Riboflavin 4.93583e-05 g/l Thiamine HCl 4.93583e-05 g/l (DL)-alpha-Lipoic acid 4.93583e-05 g/l Folic acid 1.97433e-05 g/l Biotin 1.97433e-05 g/l Na2WO4 x 2 H2O 3.94867e-06 g/l Na2SeO3 x 5 H2O 2.9615e-06 g/l Vitamin B12 9.87167e-07 g/l Distilled water

culture temp

  • @ref: 1289
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1289
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1289
  • sample type: sediment
  • geographic location: Dead Sea
  • country: Israel
  • origin.country: ISR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_49733.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_270;96_22455;97_28059;98_35746;99_49733&stattab=map
  • Last taxonomy: Sporohalobacter lortetii subclade
  • 16S sequence: KJ477330
  • Sequence Identity:
  • Total samples: 83
  • soil counts: 17
  • aquatic counts: 35
  • animal counts: 28
  • plant counts: 3

Safety information

risk assessment

  • @ref: 1289
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sporohalobacter lortetii strain DSM 3070 16S ribosomal RNA gene, partial sequenceKJ4773301494ena974
20218Sporohalobacter lortetii 16S ribosomal RNAM591221539ena974
1289Sporohalobacter lortetii partial 16S rRNA gene, type strain DSM 3070T, clone 1HE5827741527ena974
1289Sporohalobacter lortetii partial 16S rRNA gene, type strain DSM 3070T, clone 2HE5827821526ena974

GC content

  • @ref: 1289
  • GC-content: 31.5

External links

@ref: 1289

culture collection no.: DSM 3070, ATCC 35059

straininfo link

  • @ref: 75481
  • straininfo: 40845

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1289Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3070)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3070
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75481Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40845.1StrainInfo: A central database for resolving microbial strain identifiers