Strain identifier

BacDive ID: 5974

Type strain: Yes

Species: Halopiger aswanensis

Strain Designation: 56, 98-618

Strain history: DSM 13151 <-- B. H. A. Rehm 56.

NCBI tax ID(s): 148449 (species)

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General

@ref: 4927

BacDive-ID: 5974

DSM-Number: 13151

keywords: genome sequence, 16S sequence, Archaea, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Halopiger aswanensis 56 is an aerobe, mesophilic, Gram-negative archaeon that was isolated from surface of hypersaline salt soil.

NCBI tax id

  • NCBI tax id: 148449
  • Matching level: species

strain history

@refhistory
4927<- B. H. A. Rehm, Univ. Münster, Germany; 98-618
67770DSM 13151 <-- B. H. A. Rehm 56.

doi: 10.13145/bacdive5974.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Natrialbales
  • family: Natrialbaceae
  • genus: Halopiger
  • species: Halopiger aswanensis
  • full scientific name: Halopiger aswanensis Hezayen et al. 2010

@ref: 4927

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Natrialbaceae

genus: Halopiger

species: Halopiger aswanensis

full scientific name: Halopiger aswanensis Hezayen et al. 2010

strain designation: 56, 98-618

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29433negative3.75 µm0.75 µmrod-shapedyes
69480negative99.913

pigmentation

  • @ref: 29433
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 4927
  • name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/372
  • composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4927positivegrowth37mesophilic
29433positivegrowth40-55thermophilic
29433positiveoptimum40thermophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
29433positivegrowth6-9.2alkaliphile
29433positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 29433
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no96
69480no99.966

halophily

@refsaltgrowthtested relationconcentration
29433NaClpositivegrowth>10 %
29433NaClpositiveoptimum23.75 %

observation

  • @ref: 67770
  • observation: quinones: MK-8, MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2943322599arabinose+carbon source
2943328757fructose+carbon source
2943317234glucose+carbon source
2943317306maltose+carbon source
2943317992sucrose+carbon source
2943318222xylose+carbon source
2943317632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
2943316136hydrogen sulfideyes
2943335581indoleyes

metabolite tests

  • @ref: 29433
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
29433catalase+1.11.1.6
29433cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4927surface of hypersaline salt soilAswanEgyptEGYAfrica
67770Surface of hypersaline soil close to AswanEgyptEGYAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_187122.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17652;96_68805;97_89447;98_121720;99_187122&stattab=map
  • Last taxonomy: Halopiger
  • 16S sequence: AB663437
  • Sequence Identity:
  • Total samples: 4
  • soil counts: 4

Safety information

risk assessment

  • @ref: 4927
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halopiger aswanensis gene for 16S rRNA, complete sequence, strain: JCM 11628AB6634371471ena148449
4927Halopiger aswanensis strain 56 16S ribosomal RNA gene, partial sequenceAF3337591456ena148449

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Halopiger aswanensis DSM 131512636416066draftimg148449
67770Halopiger aswanensis DSM 13151GCA_003610195scaffoldncbi148449
66792Halopiger aswanensis strain DSM 13151148449.3wgspatric148449

GC content

@refGC-contentmethod
492767.1high performance liquid chromatography (HPLC)
2943367.1

Genome-based predictions

predictions

  • trait: spore-forming
  • prediction: no
  • confidence: 96
  • training_data: no

External links

@ref: 4927

culture collection no.: DSM 13151, JCM 11628

straininfo link

  • @ref: 75463
  • straininfo: 407011

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19654343Halopiger aswanensis sp. nov., a polymer-producing and extremely halophilic archaeon isolated from hypersaline soil.Hezayen FF, Gutierrez MC, Steinbuchel A, Tindall BJ, Rehm BHAInt J Syst Evol Microbiol10.1099/ijs.0.013078-02009Biopolymers/*metabolism, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Halobacteriaceae/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Soil MicrobiologyMetabolism
Phylogeny27562780Halopiger thermotolerans sp. nov., a thermo-tolerant haloarchaeon isolated from commercial salt.Minegishi H, Shimogaki R, Enomoto S, Echigo A, Kondo Y, Nagaoka S, Shimane Y, Kamekura M, Itoh T, Ohkuma M, Nunoura T, Takai K, Usami RInt J Syst Evol Microbiol10.1099/ijsem.0.0014552016Australia, Base Composition, DNA, Archaeal/genetics, Genes, Archaeal, Halobacteriaceae/*classification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium ChlorideTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
4927Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13151)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13151
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29433Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125839
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75463Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID407011.1StrainInfo: A central database for resolving microbial strain identifiers