Strain identifier

BacDive ID: 59

Type strain: Yes

Species: Rhodopila globiformis

Strain Designation: 7950

Strain history: CIP <- 2003, DSMZ <- N. Pfennig: strain 7950

NCBI tax ID(s): 1071 (species)

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General

@ref: 2128

BacDive-ID: 59

DSM-Number: 161

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Rhodopila globiformis 7950 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from sulfur spring.

NCBI tax id

  • NCBI tax id: 1071
  • Matching level: species

strain history

@refhistory
2128<- N. Pfennig, 7950
119646CIP <- 2003, DSMZ <- N. Pfennig: strain 7950

doi: 10.13145/bacdive59.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Rhodopila
  • species: Rhodopila globiformis
  • full scientific name: Rhodopila globiformis (Pfennig 1974) Imhoff et al. 1984
  • synonyms

    • @ref: 20215
    • synonym: Rhodopseudomonas globiformis

@ref: 2128

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Rhodopila

species: Rhodopila globiformis

full scientific name: Rhodopila globiformis (Pfennig 1974) Imhoff et al. 1984

strain designation: 7950

type strain: yes

Morphology

cell morphology

  • @ref: 119646
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2128RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM (DSMZ Medium 25)yeshttps://mediadive.dsmz.de/medium/25Name: RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM (DSMZ Medium 25) Composition: Mannitol 1.5 g/l Na-gluconate 0.5 g/l KH2PO4 0.5 g/l MgSO4 x 7 H2O 0.4 g/l NaCl 0.4 g/l NH4Cl 0.4 g/l Yeast extract 0.25 g/l Sodium thiosulfate pentahydrate 0.2 g/l CaCl2 x 2 H2O 0.05 g/l Fe(III) citrate 0.005 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l p-Aminobenzoic acid 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Biotin 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
36597MEDIUM 462 - for Rodophila globiformisyesDistilled water make up to (1000.000 ml);Sodium chloride (0.400 g);Magnesium sulphate heptahydrate (0.400 g);Calcium chloride dihydrate (0.050 g);Yeast extract (0.250 g);Potassium di-hydrogen phosphate (0.500 g);Ammonium chloride (0.400 g);Mannitol (1.500
119646CIP Medium 462yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=462

culture temp

@refgrowthtypetemperaturerange
2128positivegrowth25mesophilic
36597positivegrowth25mesophilic
119646positivegrowth25mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2128
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2128sulfur springWyoming, Yellowstone Natl. ParkUSAUSANorth America
119646Environment, Sulfur springUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Spring
#Condition#Sulfuric

taxonmaps

  • @ref: 69479
  • File name: preview.99_88374.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_36667;97_46587;98_61044;99_88374&stattab=map
  • Last taxonomy: Rhodopila globiformis subclade
  • 16S sequence: D86513
  • Sequence Identity:
  • Total samples: 13690
  • soil counts: 9890
  • aquatic counts: 612
  • animal counts: 650
  • plant counts: 2538

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
21281Risk group (German classification)
1196461Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Rhodopila globiformis DNA for 16S rRNA
  • accession: D86513
  • length: 1465
  • database: ena
  • NCBI tax ID: 1071

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Rhodopila globiformis DSM 161GCA_002937115scaffoldncbi1071
66792Rhodopila globiformis strain DSM 1611071.7wgspatric1071
66792Rhodopila globiformis DSM 1612831737867draftimg1071

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno79.181no
gram-positiveno98.497no
anaerobicno80.248yes
aerobicyes52.679yes
halophileno92.4no
spore-formingno90.799no
glucose-utilyes84.016no
thermophileno94.122no
motileyes73.692no
glucose-fermentno89.918no

External links

@ref: 2128

culture collection no.: DSM 161, ATCC 35887, Pfennig 7950, CIP 107835, LMG 4312

straininfo link

  • @ref: 69743
  • straininfo: 4899

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism29423563New insights into the metabolic potential of the phototrophic purple bacterium Rhodopila globiformis DSM 161(T) from its draft genome sequence and evidence for a vanadium-dependent nitrogenase.Imhoff JF, Rahn T, Kunzel S, Neulinger SCArch Microbiol10.1007/s00203-018-1489-z2018Acetobacteraceae/*genetics/isolation & purification/*metabolism/radiation effects, Bacterial Proteins/genetics/*metabolism, Base Composition, Base Sequence, Chromosome Mapping, *Genome, Bacterial, Hot Springs/microbiology, Nitrogenase/genetics/*metabolism, Photosynthetic Reaction Center Complex Proteins/genetics/metabolism, Phototrophic Processes, Vanadium/metabolismGenetics
Phylogeny31355740Acidibrevibacterium fodinaquatile gen. nov., sp. nov., isolated from acidic mine drainage.Muhadesi JB, Huang Y, Wang BJ, Jiang CY, Liu SJInt J Syst Evol Microbiol10.1099/ijsem.0.0036182019Acetobacteraceae/*classification/isolation & purification, Acids, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Mining, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2128Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 161)https://www.dsmz.de/collection/catalogue/details/culture/DSM-161
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36597Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5390
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69743Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4899.1StrainInfo: A central database for resolving microbial strain identifiers
119646Curators of the CIPCollection of Institut Pasteur (CIP 107835)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107835