Strain identifier

BacDive ID: 5898

Type strain: Yes

Species: Natronomonas moolapensis

Strain Designation: 8.8.11, CSW8.8.11

Strain history: D. Burns 8.8.11.

NCBI tax ID(s): 268739 (strain), 416273 (species)

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General

@ref: 7652

BacDive-ID: 5898

DSM-Number: 18674

keywords: genome sequence, 16S sequence, Archaea, aerobe, mesophilic, Gram-negative, motile, coccus-shaped

description: Natronomonas moolapensis 8.8.11 is an aerobe, mesophilic, Gram-negative archaeon that was isolated from solar saltern crystallizer pond.

NCBI tax id

NCBI tax idMatching level
416273species
268739strain

strain history

@refhistory
7652<- D. G. Burns, Univ. Melbourne, Australia; 8.8.11
67770D. Burns 8.8.11.

doi: 10.13145/bacdive5898.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Halobacteriales
  • family: Halobacteriaceae
  • genus: Natronomonas
  • species: Natronomonas moolapensis
  • full scientific name: Natronomonas moolapensis Burns et al. 2010

@ref: 7652

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Halobacteriaceae

genus: Natronomonas

species: Natronomonas moolapensis

full scientific name: Natronomonas moolapensis Burns et al. 2010

strain designation: 8.8.11, CSW8.8.11

type strain: yes

Morphology

cell morphology

  • @ref: 29282
  • gram stain: negative
  • cell width: 1.2 µm
  • cell shape: coccus-shaped
  • motility: yes

pigmentation

  • @ref: 29282
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 7652
  • name: HALOQUADRATUM WALSBYI MEDIUM (DSMZ Medium 1091)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1091
  • composition: Name: HALOQUADRATUM WALSBYI MEDIUM (DSMZ Medium 1091) Composition: NaCl 195.0 g/l MgSO4 x 7 H2O 50.0 g/l MgCl2 x 6 H2O 35.0 g/l KCl 5.0 g/l Tris buffer 2.4228 g/l Sodium pyruvate 1.0 g/l NaNO3 1.0 g/l CaCl2 x 2 H2O 0.5 g/l NaHCO3 0.25 g/l KH2PO4 0.05 g/l Yeast extract 0.05 g/l NH4Cl 0.03 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7652positivegrowth37mesophilic
29282positivegrowth25-45
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
29282positivegrowth5.5-8.5alkaliphile
29282positiveoptimum7.25

Physiology and metabolism

oxygen tolerance

  • @ref: 29282
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
29282NaClpositivegrowth14-36
29282NaClpositiveoptimum8-20 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2928230089acetate+carbon source
2928216236ethanol+carbon source
2928217754glycerol+carbon source
2928224996lactate+carbon source
2928217272propionate+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
7652solar saltern crystallizer pondGeelong, Moolap, Cheetham Salt Works (38°09.841 S, 144°25.274 E)AustraliaAUSAustralia and Oceania-38.1639144.421
67770Marine solar saltern crystallizer at MoolapVictoriaAustraliaAUSAustralia and Oceania

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Pond (small)
#Condition#Saline

Safety information

risk assessment

  • @ref: 7652
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Natronomonas moolapensis gene for 16S ribosomal RNA, partial sequenceAB5761271467ena268739
20218Natronomonas moolapensis gene for 16S rRNA, complete sequence, strain: JCM 14361AB6634311467ena268739
7652Natronomonas moolapensis 8.8.11 strain CSW8.8.11 16S ribosomal RNA gene, partial sequenceAY4986451311ena268739

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Natronomonas moolapensis 8.8.11GCA_000591055completencbi268739
66792Natronomonas moolapensis 8.8.11, DSM 186742540341082completeimg268739
66792Natronomonas moolapensis 8.8.11268739.6completepatric268739

GC content

@refGC-contentmethod
765263.4
2928263-64
6777063.4high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno66.535no
flagellatedno95.489no
gram-positiveno97.587yes
anaerobicno96.289no
halophileyes91.091yes
spore-formingno93.705no
glucose-utilyes85.123no
aerobicyes85.153yes
thermophileno87.762yes
glucose-fermentno81.577no

External links

@ref: 7652

culture collection no.: DSM 18674, CECT 7526, JCM 14361

straininfo link

  • @ref: 75378
  • straininfo: 370211

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19667395Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas.Burns DG, Janssen PH, Itoh T, Minegishi H, Usami R, Kamekura M, Dyall-Smith MLInt J Syst Evol Microbiol10.1099/ijs.0.010132-02009Crystallization, DNA, Archaeal/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Halobacteriaceae/*classification/genetics/*isolation & purification/physiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, *Sodium Chloride, Species SpecificityGenetics
23516216Genome of the haloarchaeon Natronomonas moolapensis, a neutrophilic member of a previously haloalkaliphilic genus.Dyall-Smith ML, Pfeiffer F, Oberwinkler T, Klee K, Rampp M, Palm P, Gross K, Schuster SC, Oesterhelt DGenome Announc10.1128/genomeA.00095-132013
Phylogeny23851717Natronomonas gomsonensis sp. nov., isolated from a solar saltern.Kim TY, Kim SJ, Park SJ, Kim JG, Cha IT, Jung MY, Lee SA, Roh SW, Yim KJ, Itoh T, Rhee SKAntonie Van Leeuwenhoek10.1007/s10482-013-9970-92013Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/microbiology, Halobacteriaceae/*classification/genetics/*isolation & purification/physiology, Hydrogen-Ion Concentration, Korea, Magnesium Chloride/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7652Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18674)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18674
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29282Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125698
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
75378Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID370211.1StrainInfo: A central database for resolving microbial strain identifiers