Strain identifier
BacDive ID: 5893
Type strain: 
Species: Natrinema longum
Strain Designation: EJ-57
Strain history: A. Ventosa EJ-57.
NCBI tax ID(s): 373386 (species)
version 8.1 (current version)
General
@ref: 7418
BacDive-ID: 5893
DSM-Number: 18194
keywords: genome sequence, 16S sequence, Archaea, mesophilic, Gram-negative
description: Natrinema longum EJ-57 is a mesophilic, Gram-negative archaeon that was isolated from sediment sample of a saltern.
NCBI tax id
- NCBI tax id: 373386
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7418 | <- A. Ventosa, Univ. Seville, Dept. Microbiol. Parasitol., Spain; EJ-57 <- A. M. Castillo et al. |
| 67770 | A. Ventosa EJ-57. |
doi: 10.13145/bacdive5893.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Halobacteria
- order: Natrialbales
- family: Natrialbaceae
- genus: Natrinema
- species: Natrinema longum
- full scientific name: Natrinema longum (Cui et al. 2006) de la Haba et al. 2022
synonyms
@ref synonym 20215 Haloterrigena longa 20215 Natrinema ejinorense
@ref: 7418
domain: Archaea
phylum: Euryarchaeota
class: Halobacteria
order: Halobacteriales
family: Natrialbaceae
genus: Natrinema
species: Natrinema ejinorense
full scientific name: Natrinema ejinorense Castillo et al. 2006
strain designation: EJ-57
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.977
Culture and growth conditions
culture medium
- @ref: 7418
- name: MEDIUM FOR HALOPHILIC ARCHAEA (DSMZ Medium 1184)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1184
- composition: Name: MEDIUM FOR HALOPHILIC ARCHAEA (DSMZ Medium 1184) Composition: NaCl 195.0 g/l MgSO4 x 7 H2O 50.8 g/l MgCl2 x 6 H2O 32.5 g/l Agar 20.0 g/l KCl 5.0 g/l Yeast extract 5.0 g/l CaCl2 x 2 H2O 0.8 g/l NaBr 0.6 g/l NaHCO3 0.16 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7418 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.992 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 7418 | sediment sample of a saltern | Inner Mongolia, Lake Ejinor (45° 14' N 116° 31' E) | China | CHN | Asia | 45.2333 | 116.517 |
| 67770 | Sediments of a saltern beside Lake Ejinor | Inner Mongolia | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Sediment |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_191396.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17652;96_69228;97_89926;98_122263;99_191396&stattab=map
- Last taxonomy: Natrialbaceae
- 16S sequence: AB663461
- Sequence Identity:
- Total samples: 1
- aquatic counts: 1
Safety information
risk assessment
- @ref: 7418
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Natrinema ejinorense gene for 16S ribosomal RNA, partial sequence, strain: JCM 13890 | AB477229 | 1434 | ena | 373386 |
| 20218 | Natrinema ejinorense gene for 16S rRNA, complete sequence, strain: JCM 13890 | AB663461 | 1474 | ena | 373386 |
| 7418 | Natrinema ejinorense 16S rRNA gene, type strain EJ-57T | AM231733 | 1418 | ena | 373386 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 67770 | Natrinema ejinorense JCM 13890 | GCA_002494345 | contig | ncbi | 373386 |
| 66792 | Natrinema ejinorense strain JCM 13890 | 373386.4 | wgs | patric | 373386 |
GC content
- @ref: 7418
- GC-content: 64.7
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| gram-positive | no | 96.306 | no |
| anaerobic | no | 96.93 | no |
| halophile | yes | 90.101 | no |
| spore-forming | no | 91.893 | no |
| glucose-util | yes | 89.211 | no |
| aerobic | yes | 87.937 | no |
| motile | no | 80.243 | no |
| flagellated | no | 96.002 | no |
| thermophile | no | 84.874 | yes |
| glucose-ferment | no | 86.254 | no |
External links
@ref: 7418
culture collection no.: DSM 18194, CECT 7144, CGMCC 1.6202, JCM 13890
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 17082411 | Natrinema ejinorense sp. nov., isolated from a saline lake in Inner Mongolia, China. | Castillo AM, Gutierrez MC, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa A | Int J Syst Evol Microbiol | 10.1099/ijs.0.64421-0 | 2006 | Base Composition, Carbohydrate Metabolism, China, DNA, Archaeal/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Gelatin/metabolism, Genes, rRNA, Halobacteriaceae/*classification/cytology/*isolation & purification/physiology, Hydrogen Sulfide/metabolism, Hydrogen-Ion Concentration, Lipids/analysis, Molecular Sequence Data, Movement, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Starch/metabolism, Temperature, *Water Microbiology | Metabolism |
| Phylogeny | 22817877 | Natrinema salaciae sp. nov., a halophilic archaeon isolated from the deep, hypersaline anoxic Lake Medee in the Eastern Mediterranean Sea. | Albuquerque L, Taborda M, La Cono V, Yakimov M, da Costa MS | Syst Appl Microbiol | 10.1016/j.syapm.2012.06.005 | 2012 | Anaerobiosis, Genes, Archaeal/genetics, Halobacteriaceae/*classification/genetics/*isolation & purification, Italy, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Salts, Water Microbiology | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 7418 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18194) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18194 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 |