Strain identifier

BacDive ID: 5694

Type strain: Yes

Species: Chryseobacterium hungaricum

Strain Designation: CHB-20p

Strain history: CIP <- 2010, DSMZ <- S. Szoboszlay, Agruniver Holding, Hungary: strain CHB-20p

NCBI tax ID(s): 454006 (species)

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General

@ref: 8254

BacDive-ID: 5694

DSM-Number: 19684

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Chryseobacterium hungaricum CHB-20p is an aerobe, mesophilic, Gram-negative bacterium that was isolated from hydrocarbon-contaminated soil.

NCBI tax id

  • NCBI tax id: 454006
  • Matching level: species

strain history

@refhistory
8254<- S. Szoboszlay, Agruniver Holding, Environmental Management, Res. and Technol. Development Ltd., Hungary; CHB-20p <- S. Szoboszlay and B. Kriszt
119990CIP <- 2010, DSMZ <- S. Szoboszlay, Agruniver Holding, Hungary: strain CHB-20p

doi: 10.13145/bacdive5694.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Weeksellaceae
  • genus: Chryseobacterium
  • species: Chryseobacterium hungaricum
  • full scientific name: Chryseobacterium hungaricum Szoboszlay et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Epilithonimonas hungarica

@ref: 8254

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Weeksellaceae

genus: Chryseobacterium

species: Chryseobacterium hungaricum

full scientific name: Chryseobacterium hungaricum Szoboszlay et al. 2008

strain designation: CHB-20p

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32652negative0.9 µm0.5 µmrod-shapedno
69480no91.041
69480negative99.987
119990negativerod-shapedyes

pigmentation

  • @ref: 32652
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8254NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39756MEDIUM 328- for nutrient agaryesDistilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
119990CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328

culture temp

@refgrowthtypetemperaturerange
8254positivegrowth28mesophilic
32652positivegrowth05-37
32652positiveoptimum28-30mesophilic
39756positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
32652positivegrowth06-10alkaliphile
32652positiveoptimum6.5-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32652aerobe
119990obligate aerobe

spore formation

@refspore formationconfidence
32652no
69481no100
69480no99.994

halophily

@refsaltgrowthtested relationconcentration
32652NaClpositivegrowth0-2 %
32652NaClpositiveoptimum0-1 %

observation

  • @ref: 32652
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3265217234glucose+carbon source
3265217306maltose+carbon source
3265237684mannose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11999017632nitrate+reduction
11999016301nitrite-reduction

metabolite production

  • @ref: 119990
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
32652catalase+1.11.1.6
32652cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119990oxidase+
119990catalase+1.11.1.6
119990urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119990-+++-++---++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119990-----+/-----+/--+/--+/--------+/-+/-+/-+/-+/-+/----+/----+/-+/--+/-----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8254hydrocarbon-contaminated soilTököl regionHungaryHUNEurope
119990Environment, Hydrocarbon-contaminated soilTököl regionHungaryHUNEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
82541Risk group (German classification)
1199901Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8254
  • description: Chryseobacterium hungaricum strain CHB-20p 16S ribosomal RNA gene, partial sequence
  • accession: EF685359
  • length: 1406
  • database: ena
  • NCBI tax ID: 454006

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Epilithonimonas hungarica DSM 19684GCA_900102265contigncbi454006
66792Chryseobacterium hungaricum strain DSM 19684454006.31wgspatric454006
66792Epilithonimonas hungarica DSM 196842684622908draftimg454006

GC content

  • @ref: 8254
  • GC-content: 37.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.454yes
anaerobicno99.454yes
halophileno95.53yes
spore-formingno96.092yes
glucose-utilyes86.458yes
aerobicyes89.513no
flagellatedno95.926yes
thermophileno99.584yes
motileno91.81yes
glucose-fermentno89.931no

External links

@ref: 8254

culture collection no.: DSM 19684, NCAIM B2269, NCAIM B.02269, CIP 110178

straininfo link

  • @ref: 75176
  • straininfo: 360268

literature

  • topic: Phylogeny
  • Pubmed-ID: 19060052
  • title: Chryseobacterium hungaricum sp. nov., isolated from hydrocarbon-contaminated soil.
  • authors: Szoboszlay S, Atzel B, Kukolya J, Toth EM, Marialigeti K, Schumann P, Kriszt B
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65847-0
  • year: 2008
  • mesh: Chryseobacterium/chemistry/*classification/genetics/*physiology, Fatty Acids/analysis, Hydrocarbons, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Soil Pollutants, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
8254Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19684)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19684
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32652Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2886328776041
39756Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8008
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75176Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID360268.1StrainInfo: A central database for resolving microbial strain identifiers
119990Curators of the CIPCollection of Institut Pasteur (CIP 110178)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110178