Strain identifier

BacDive ID: 5616

Type strain: Yes

Species: Mesonia algae

Strain Designation: M-2Alg 33-2

Strain history: CIP <- 2003, CCUG <- 2002, KMM

NCBI tax ID(s): 213248 (species)

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General

@ref: 5875

BacDive-ID: 5616

DSM-Number: 15361

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, chemoorganotroph, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Mesonia algae M-2Alg 33-2 is an obligate aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from green alga Acrosiphonia sonderi.

NCBI tax id

  • NCBI tax id: 213248
  • Matching level: species

strain history

@refhistory
5875<- O. I. Nedashkovskaya, KMM; M-2Alg 33-2
116219CIP <- 2003, CCUG <- 2002, KMM

doi: 10.13145/bacdive5616.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Mesonia
  • species: Mesonia algae
  • full scientific name: Mesonia algae Nedashkovskaya et al. 2003

@ref: 5875

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Mesonia

species: Mesonia algae

full scientific name: Mesonia algae Nedashkovskaya et al. 2003

strain designation: M-2Alg 33-2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43388negative1.6-2.3 µm0.4-0.5 µmrod-shapedno
69480negative99.994
116219negativerod-shapedno

colony morphology

@refcolony sizecolony colorcolony shapemedium used
433882-4 mmyellowcircularMarine agar
116219

pigmentation

  • @ref: 116219
  • production: no
  • name: Flexirubin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5875BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
32901Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
43388Marine agar (MA)yes
116219CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5875positivegrowth25mesophilic
32901positivegrowth25mesophilic
43388positiveoptimum21-23psychrophilic
43388positivegrowth4-34
43388nogrowth37mesophilic
57504nogrowth37mesophilic
57504positivegrowth25-28mesophilic
116219positivegrowth5-30
116219nogrowth37mesophilic
116219nogrowth41thermophilic
116219nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43388obligate aerobe
57504aerobe
116219obligate aerobe

nutrition type

  • @ref: 43388
  • type: chemoorganotroph

spore formation

@reftype of sporespore formationconfidence
43388endosporeno
69481no100
69480no99.994

halophily

@refsaltgrowthtested relationconcentration
43388NaClpositivegrowth1-15 %
116219NaClpositivegrowth2-10 %
116219NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4338817128adipate-assimilation
4338815963ribitol-builds acid from
433882509agar-hydrolysis
4338858187alginate-hydrolysis
4338822599arabinose-builds acid from
4338822599arabinose-assimilation
4338827689decanoate-assimilation
4338885146carboxymethylcellulose-hydrolysis
43388casein+degradation
43388casein+hydrolysis
4338817057cellobiose-builds acid from
4338862968cellulose-hydrolysis
4338817029chitin-hydrolysis
4338816947citrate-builds acid from
4338816947citrate-assimilation
4338825115malate-assimilation
4338816991dna-hydrolysis
4338816813galactitol-builds acid from
4338833984fucose-builds acid from
4338828260galactose-builds acid from
433885291gelatin+degradation
433885291gelatin+hydrolysis
4338824265gluconate-assimilation
4338817234glucose-builds acid from
4338817234glucose-assimilation
4338817754glycerol-builds acid from
4338817268myo-inositol-builds acid from
4338817268myo-inositol-assimilation
4338817716lactose-builds acid from
4338817716lactose-assimilation
4338815792malonate-assimilation
4338817306maltose-builds acid from
4338829864mannitol-builds acid from
4338829864mannitol-assimilation
4338837684mannose-builds acid from
4338837684mannose-assimilation
4338828053melibiose-builds acid from
43388506227N-acetylglucosamine-assimilation
4338817632nitrate-reduction
4338818401phenylacetate-assimilation
4338816634raffinose-builds acid from
4338826546rhamnose-builds acid from
4338830911sorbitol-assimilation
4338828017starch-hydrolysis
4338817992sucrose-builds acid from
4338817992sucrose-assimilation
4338827897tryptophan-energy source
4338853424tween 20+degradation
4338853423tween 40+degradation
4338853426tween 80+degradation
4338816199urea-hydrolysis
4338818222xylose-builds acid from
11621916947citrate-carbon source
1162194853esculin+hydrolysis
116219606565hippurate-hydrolysis
11621917632nitrate-builds gas from
11621917632nitrate-reduction
11621916301nitrite-builds gas from
11621916301nitrite+reduction
11621915792malonate-assimilation

metabolite production

@refChebi-IDmetaboliteproduction
4338816136hydrogen sulfideyes
4338815688acetoinno
4338835581indoleno
11621935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
4338815688acetoin-
4338835581indole-
11621915688acetoin-
11621917234glucose-

enzymes

@refvalueactivityec
43388cytochrome oxidase+1.9.3.1
43388catalase+1.11.1.6
43388alkaline phosphatase+3.1.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116219oxidase+
116219beta-galactosidase+3.2.1.23
116219alcohol dehydrogenase-1.1.1.1
116219gelatinase+/-
116219amylase-
116219DNase+
116219caseinase+3.4.21.50
116219catalase+1.11.1.6
116219tween esterase-
116219gamma-glutamyltransferase+2.3.2.2
116219lecithinase-
116219lipase-
116219lysine decarboxylase-4.1.1.18
116219ornithine decarboxylase-4.1.1.17
116219phenylalanine ammonia-lyase-4.3.1.24
116219tryptophan deaminase-
116219urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116219-+++-++---++-+-+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116219+-+-+--+-+-+------+--+--+-----------------------------+--------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
5875green alga Acrosiphonia sonderiAcrosiphonia sonderiSea of Japan, Gulf of Peter the GreatRussiaRUSAsia
116219Green alga, Acrosiphonia sonderiTroitsa bay, Sea of JapanRussian FederationRUSEurope2000

isolation source categories

Cat1Cat2Cat3
#Host#Algae#Green algae
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_8724.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_52;96_4071;97_4994;98_6375;99_8724&stattab=map
  • Last taxonomy: Mesonia algae subclade
  • 16S sequence: AF536383
  • Sequence Identity:
  • Total samples: 9208
  • soil counts: 360
  • aquatic counts: 7836
  • animal counts: 964
  • plant counts: 48

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58751Risk group (German classification)
1162191Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5875
  • description: Marine bacterium KMM 3909 16S ribosomal RNA gene, partial sequence
  • accession: AF536383
  • length: 1428
  • database: ena
  • NCBI tax ID: 213248

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesonia algae DSM 15361GCA_003253545scaffoldncbi213248
66792Mesonia algae strain DSM 15361213248.10wgspatric213248
66792Mesonia algae DSM 153612593339303draftimg213248

GC content

@refGC-contentmethod
587532.7-34
4338832.7-34Thermal denaturation, fluorometry

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno92.751yes
gram-positiveno98.292yes
anaerobicno99.144yes
halophileyes72.857no
spore-formingno95.006yes
aerobicyes92.224yes
thermophileno99.623no
glucose-utilyes73.38yes
motileno83.731yes
glucose-fermentno92.577no

External links

@ref: 5875

culture collection no.: DSM 15361, CCUG 47092, KCTC 12089, KMM 3909, CIP 108200

straininfo link

  • @ref: 75099
  • straininfo: 99595

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14657131Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kutz) Kornm.Nedashkovskaya OI, Kim SB, Han SK, Lysenko AM, Rohde M, Zhukova NV, Falsen E, Frolova GM, Mikhailov VV, Bae KSInt J Syst Evol Microbiol10.1099/ijs.0.02626-02003Chlorophyta/*microbiology, DNA, Bacterial/genetics/isolation & purification, DNA, Ribosomal/genetics, Flavobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Bacterial/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Seawater/microbiologyEnzymology
Metabolism17066955[Distribution of intracellular fucoidan hydrolases among marine bacteria of the family Flavobacteriaceae].Urvantseva AM, Bakunina IIu, Nedashkovskaia OI, Kim SB, Zviagintseva TNPrikl Biokhim Mikrobiol2006Animals, Bacterial Proteins/*chemistry/metabolism, Eukaryota/microbiology, Flavobacteriaceae/chemistry/*enzymology/isolation & purification, Hydrolases/*chemistry/metabolism, Oceans and Seas, Polysaccharides/*metabolism, Sea Cucumbers/microbiology, Strongylocentrotus/microbiology, *Water MicrobiologyEnzymology
Phylogeny32441611Haloflavibacter putidus gen. nov., sp. nov., isolated from coastal seawater.Feng X, Wang YR, Zou QH, Zhang JY, Du ZJInt J Syst Evol Microbiol10.1099/ijsem.0.0042282020Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5875Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15361)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15361
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32901Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5797
43388Olga I. Nedashkovskaya, Seung Bum Kim, Suk Kyun Han, Anatoly M. Lysenko, Manfred Rohde, Natalia V. Zhukova, Enevold Falsen, Galina M. Frolova, Valery V. Mikhailov, Kyung Sook Bae10.1099/ijs.0.02626-0Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) KornmIJSEM 53: 1967-1971 200314657131
57504Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47092)https://www.ccug.se/strain?id=47092
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75099Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID99595.1StrainInfo: A central database for resolving microbial strain identifiers
116219Curators of the CIPCollection of Institut Pasteur (CIP 108200)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108200