Strain identifier
BacDive ID: 555
Type strain: 
Species: Anoxybacillus caldiproteolyticus
Strain Designation: R-35652, SF03
Strain history: <- X.-G. Chen, Nanyang Univ., Singapore; SF03
NCBI tax ID(s): 247480 (species)
version 8.1 (current version)
General
@ref: 6087
BacDive-ID: 555
DSM-Number: 15730
keywords: genome sequence, 16S sequence, Bacteria, thermophilic
description: Anoxybacillus caldiproteolyticus R-35652 is a thermophilic bacterium that was isolated from sewage sludge.
NCBI tax id
- NCBI tax id: 247480
- Matching level: species
strain history
- @ref: 6087
- history: <- X.-G. Chen, Nanyang Univ., Singapore; SF03
doi: 10.13145/bacdive555.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Anoxybacillus
- species: Anoxybacillus caldiproteolyticus
- full scientific name: Anoxybacillus caldiproteolyticus Coorevits et al. 2012
synonyms
- @ref: 20215
- synonym: Geobacillus caldoproteolyticus
@ref: 6087
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Anoxybacillus
species: Anoxybacillus caldiproteolyticus
full scientific name: Anoxybacillus caldiproteolyticus Coorevits et al. 2012
strain designation: R-35652, SF03
type strain: yes
Culture and growth conditions
culture medium
- @ref: 6087
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
- @ref: 6087
- growth: positive
- type: growth
- temperature: 55
- range: thermophilic
Isolation, sampling and environmental information
isolation
- @ref: 6087
- sample type: sewage sludge
- geographic location: Seletar water reclamation plant
- country: Singapore
- origin.country: SGP
- continent: Asia
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Sewage sludge
taxonmaps
- @ref: 69479
- File name: preview.99_101774.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_1044;97_1219;98_69454;99_101774&stattab=map
- Last taxonomy: Anoxybacillus caldiproteolyticus subclade
- 16S sequence: AY327448
- Sequence Identity:
- Total samples: 60
- soil counts: 10
- aquatic counts: 25
- animal counts: 22
- plant counts: 3
Safety information
risk assessment
- @ref: 6087
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 6087 | Geobacillus sp. SF03 16S ribosomal RNA gene, partial sequence | AY327448 | 1506 | ena | 247480 |
| 6087 | Geobacillus caldoproteolyticus partial 16S rRNA gene, strain R-35652 | FN428698 | 1504 | ena | 247480 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Anoxybacillus caldiproteolyticus DSM 15730 | GCA_013761245 | contig | ncbi | 247480 |
| 66792 | Anoxybacillus caldiproteolyticus strain DSM 15730 | 247480.4 | wgs | patric | 247480 |
| 66792 | Anoxybacillus caldiproteolyticus DSM 15730 | 2861199460 | draft | img | 247480 |
GC content
- @ref: 6087
- GC-content: 40.2
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 90.479 | no |
| gram-positive | yes | 85.824 | no |
| anaerobic | no | 96.962 | no |
| aerobic | yes | 85.813 | no |
| halophile | no | 87.35 | no |
| spore-forming | yes | 97.178 | no |
| glucose-util | yes | 90.863 | no |
| flagellated | yes | 91.346 | no |
| thermophile | yes | 99.147 | yes |
| glucose-ferment | no | 90.373 | no |
External links
@ref: 6087
culture collection no.: DSM 15730, ATCC BAA 818, LMG 26209
straininfo link
- @ref: 70233
- straininfo: 132195
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 21856988 | Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov. | Coorevits A, Dinsdale AE, Halket G, Lebbe L, De Vos P, Van Landschoot A, Logan NA | Int J Syst Evol Microbiol | 10.1099/ijs.0.030346-0 | 2011 | Aerobiosis, Bacillales/*classification/genetics/physiology, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial/cytology | Genetics |
| Phylogeny | 24052627 | Anoxybacillus calidus sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant. | Cihan AC, Cokmus C, Koc M, Ozcan B | Int J Syst Evol Microbiol | 10.1099/ijs.0.056549-0 | 2013 | Anoxybacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, *Power Plants, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Turkey, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 27126386 | Anoxybacillusgeothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station. | Filippidou S, Jaussi M, Junier T, Wunderlin T, Jeanneret N, Palmieri F, Palmieri I, Roussel-Delif L, Vieth-Hillebrand A, Vetter A, Chain PS, Regenspurg S, Junier P | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001125 | 2016 | Anoxybacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, Groundwater/*microbiology, Minerals, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Power Plants, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Phylogeny | 29931494 | Anoxybacillus sediminis sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring. | Khan IU, Habib N, Xiao M, Devi AM, Habib M, Hejazi MS, Salam N, Zhi XY, Li WJ | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1118-5 | 2018 | Anoxybacillus/classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/chemistry/*microbiology, Hot Springs/chemistry/*microbiology, Hot Temperature, Phylogeny, RNA, Ribosomal, 16S/genetics, Tibet | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 6087 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15730) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15730 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 70233 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID132195.1 | StrainInfo: A central database for resolving microbial strain identifiers |