Strain identifier

BacDive ID: 549

Type strain: Yes

Species: Alkalibacillus flavidus

Strain Designation: ISL-17

Strain history: <- JH Yoon, KRIBB

NCBI tax ID(s): 546021 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 17256

BacDive-ID: 549

DSM-Number: 23520

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Alkalibacillus flavidus ISL-17 is an aerobe, spore-forming, mesophilic bacterium that was isolated from Sediment, marine solar saltern.

NCBI tax id

  • NCBI tax id: 546021
  • Matching level: species

strain history

@refhistory
17256<- CCUG; CCUG 56753 <- J.-H. Yoon, KRIBB; ISL-17
67771<- JH Yoon, KRIBB

doi: 10.13145/bacdive549.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Alkalibacillus
  • species: Alkalibacillus flavidus
  • full scientific name: Alkalibacillus flavidus Yoon et al. 2010

@ref: 17256

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Alkalibacillus

species: Alkalibacillus flavidus

full scientific name: Alkalibacillus flavidus Yoon et al. 2010

strain designation: ISL-17

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
29431positive5.15 µm0.3 µmrod-shapedyes
67771rod-shapedyesperitrichous
67771positive

colony morphology

  • @ref: 61075
  • incubation period: 5 days

pigmentation

  • @ref: 29431
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 17256
  • name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/220
  • composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17256positivegrowth35mesophilic
29431positivegrowth10-55
29431positiveoptimum37mesophilic
61075positivegrowth37mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
29431positivegrowth5.5-9.5alkaliphile
29431positiveoptimum8.75

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29431aerobe
61075aerobe
67771aerobe

spore formation

@refspore formationtype of spore
29431yes
67771yesendospore

halophily

@refsaltgrowthtested relationconcentration
29431NaClpositivegrowth04-26 %
29431NaClpositiveoptimum10 %

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2943117306maltose+carbon source
2943133942ribose+carbon source

enzymes

@refvalueactivityec
29431alkaline phosphatase+3.1.3.1
29431catalase+1.11.1.6
29431cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
17256Sediment, marine solar salternYellow SeaRepublic of KoreaKORAsia
61075Marine solar saltern sedimentYellow SeaRepublic of KoreaKORAsia2006
67771From sediment, marine solar salternYellow SeaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_96553.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_15055;97_18382;98_22890;99_96553&stattab=map
  • Last taxonomy: Alkalibacillus flavidus
  • 16S sequence: EU874387
  • Sequence Identity:
  • Total samples: 12
  • aquatic counts: 11
  • animal counts: 1

Safety information

risk assessment

  • @ref: 17256
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17256
  • description: Alkalibacillus flavidus strain ISL-17 16S ribosomal RNA gene, partial sequence
  • accession: EU874387
  • length: 1520
  • database: ena
  • NCBI tax ID: 546021

Genome sequences

  • @ref: 66792
  • description: Alkalibacillus flavidus DSM 23520
  • accession: 2928986558
  • assembly level: draft
  • database: img
  • NCBI tax ID: 546021

GC content

@refGC-contentmethod
1725648.1
6777148.1high performance liquid chromatography (HPLC)

External links

@ref: 17256

culture collection no.: DSM 23520, CCUG 56753, KCTC 13258, ISL -17

straininfo link

  • @ref: 70227
  • straininfo: 399242

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19651725Alkalibacillus flavidus sp. nov., isolated from a marine solar saltern.Yoon JH, Kang SJ, Jung YT, Lee MH, Oh TKInt J Syst Evol Microbiol10.1099/ijs.0.013003-02009Bacillaceae/*classification/genetics, Base Composition, DNA, Bacterial/chemistry, Korea, Molecular Sequence Data, Oceans and Seas, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Salts/chemistry, Seawater/*microbiology, Sequence Homology, Nucleic Acid, Sodium Chloride, *Water MicrobiologyGenetics
Phylogeny24670895Alkalibacillus almallahensis sp. nov., a halophilic bacterium isolated from an inland solar saltern.Perez-Davo A, Aguilera M, Ramos-Cormenzana A, Monteoliva-Sanchez MInt J Syst Evol Microbiol10.1099/ijs.0.059121-02014Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Sodium Chloride, Spain, Vitamin K 2/analogs & derivatives/chemistry, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17256Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23520)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23520
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29431Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2583728776041
61075Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56753)https://www.ccug.se/strain?id=56753
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70227Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID399242.1StrainInfo: A central database for resolving microbial strain identifiers