Strain identifier
BacDive ID: 545
Type strain: 
Species: Alkalibacillus filiformis
Strain Designation: 4AG
Strain history: DSM 15448 <-- A. Gambacorta 4AG <-- I. Romano.
NCBI tax ID(s): 200990 (species)
version 9 (current version)
General
@ref: 5807
BacDive-ID: 545
DSM-Number: 15448
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped
description: Alkalibacillus filiformis 4AG is an aerobe, spore-forming, Gram-positive bacterium that was isolated from water and mud.
NCBI tax id
- NCBI tax id: 200990
- Matching level: species
strain history
| @ref | history |
|---|---|
| 5807 | <- A. Gambacorta, ICB-CNR, Pozzuoli; 4AG <- I. Romano |
| 67770 | DSM 15448 <-- A. Gambacorta 4AG <-- I. Romano. |
doi: 10.13145/bacdive545.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Alkalibacillus
- species: Alkalibacillus filiformis
- full scientific name: Alkalibacillus filiformis Romano et al. 2005
@ref: 5807
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Alkalibacillus
species: Alkalibacillus filiformis
full scientific name: Alkalibacillus filiformis Romano et al. 2005
strain designation: 4AG
type strain: yes
Morphology
cell morphology
- @ref: 31594
- gram stain: positive
- cell length: 10 µm
- cell width: 0.275 µm
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
- @ref: 5807
- name: BACILLUS FILIFORMIS MEDIUM (DSMZ Medium 992)
- growth: yes
- link: https://mediadive.dsmz.de/medium/992
- composition: Name: BACILLUS FILIFORMIS MEDIUM (DSMZ Medium 992) Composition: NaCl 100.0 g/l Yeast extract 10.0 g/l Na2CO3 3.0 g/l Sodium citrate 3.0 g/l KCl 2.0 g/l MgSO4 x 7 H2O 1.0 g/l FeSO4 x 7 H2O 0.05 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 5807 | positive | growth | 37 |
| 31594 | positive | growth | 15-45 |
| 31594 | positive | optimum | 30 |
| 67770 | positive | growth | 30 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31594 | positive | growth | 7.0-10.0 | alkaliphile |
| 31594 | positive | optimum | 9 |
Physiology and metabolism
oxygen tolerance
- @ref: 31594
- oxygen tolerance: aerobe
spore formation
- @ref: 31594
- spore formation: yes
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 31594 | NaCl | positive | growth | <18 % |
| 31594 | NaCl | positive | optimum | 10 % |
observation
| @ref | observation |
|---|---|
| 31594 | aggregates in clumps |
| 67770 | quinones: MK-7, DMK-6 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31594 | 17234 | glucose | + | carbon source |
| 31594 | 29987 | glutamate | + | carbon source |
| 31594 | 15428 | glycine | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 31594 | alpha-galactosidase | + | 3.2.1.22 |
| 31594 | catalase | + | 1.11.1.6 |
| 31594 | gelatinase | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 5807 | water and mud | Malvizzia (AV)-Campania | Italy | ITA | Europe |
| 67770 | Mineral pool located in Malvizza | Campania | Italy | ITA | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Mud (Sludge)
Safety information
risk assessment
- @ref: 5807
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5807
- description: Alkalibacillus filiformis 16S rRNA gene, type strain 4AG
- accession: AJ493661
- length: 1442
- database: nuccore
- NCBI tax ID: 200990
Genome sequences
- @ref: 66792
- description: Alkalibacillus filiformis DSM 15448
- accession: 2926601655
- assembly level: draft
- database: img
- NCBI tax ID: 200990
GC content
| @ref | GC-content | method |
|---|---|---|
| 5807 | 39.5 | |
| 67770 | 39.5 | high performance liquid chromatography (HPLC) |
External links
@ref: 5807
culture collection no.: DSM 15448, ATCC BAA-956, CIP 109217, JCM 13893
straininfo link
- @ref: 70223
- straininfo: 281440
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16280503 | Alkalibacillus filiformis sp. nov., isolated from a mineral pool in Campania, Italy. | Romano I, Lama L, Nicolaus B, Gambacorta A, Giordano A | Int J Syst Evol Microbiol | 10.1099/ijs.0.63864-0 | 2005 | Bacillaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/chemistry/*microbiology, Italy, *Minerals, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/analysis/genetics, Sequence Analysis, DNA | Genetics |
| Phylogeny | 17392204 | Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan. | Usami R, Echigo A, Fukushima T, Mizuki T, Yoshida Y, Kamekura M | Int J Syst Evol Microbiol | 10.1099/ijs.0.64713-0 | 2007 | Bacillaceae/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Japan, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride, *Soil Microbiology, Trees | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 5807 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15448) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15448 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 31594 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27883 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 70223 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID281440.1 | StrainInfo: A central database for resolving microbial strain identifiers |