Strain identifier

BacDive ID: 5378

Type strain: Yes

Species: Kandleria vitulina

Strain Designation: RL 2, T 185

Strain history: CIP <- 1988, NCFB <- M.J. Latham: strain RL 2 <- M.P. Bryant: strain T 185

NCBI tax ID(s): 1410657 (strain), 1630 (species)

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General

@ref: 8791

BacDive-ID: 5378

DSM-Number: 20405

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive

description: Kandleria vitulina RL 2 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from calf rumen.

NCBI tax id

NCBI tax idMatching level
1410657strain
1630species

strain history

@refhistory
8791<- ATCC <- M.E. Sharpe, RL 2
67770ATCC 27783 <-- M. E. Sharpe RL 2 <-- M. P. Bryant.
123879CIP <- 1988, NCFB <- M.J. Latham: strain RL 2 <- M.P. Bryant: strain T 185

doi: 10.13145/bacdive5378.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Erysipelotrichia
  • order: Erysipelotrichales
  • family: Coprobacillaceae
  • genus: Kandleria
  • species: Kandleria vitulina
  • full scientific name: Kandleria vitulina (Sharpe et al. 1973) Salvetti et al. 2011

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus vitulinus

@ref: 8791

domain: Bacteria

phylum: Firmicutes

class: Erysipelotrichia

order: Erysipelotrichales

family: Coprobacillaceae

genus: Kandleria

species: Kandleria vitulina

full scientific name: Kandleria vitulina (Sharpe et al. 1973) Salvetti et al. 2011

strain designation: RL 2, T 185

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shape
69480positive99.72
123879positiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8791BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)yeshttps://mediadive.dsmz.de/medium/58Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
8791MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232)yeshttps://mediadive.dsmz.de/medium/232Name: MRS MEDIUM WITH CYSTEINE (DSMZ Medium 232) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)2 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37121MEDIUM 43 - for Lactobacillus hamsteri and Lactobacillus ruminisyesDistilled water make up to (900.000 ml);Man Rogosa Sharp broth (55.000 g);Cysteine hydrochloride 5 % solution - M0172 (100.000 ml)
123879CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
8791positivegrowth37mesophilic
37121positivegrowth37mesophilic
50919positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8791anaerobe
50919anaerobe
69480anaerobe98.651
123879anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.998

murein

  • @ref: 8791
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12387917632nitrate-reduction
12387916301nitrite-reduction
12387917632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6838035581indole-
12387915688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
123879alcohol dehydrogenase-1.1.1.1
123879lysine decarboxylase-4.1.1.18
123879ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
50919----------++--------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
50919--+-+-+----------------------

Isolation, sampling and environmental information

isolation

@refsample type
8791calf rumen
50919Calf rumen
67770Calf rumen
123879Animal, Calf rumen

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Rumen

taxonmaps

  • @ref: 69479
  • File name: preview.99_2929.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_300;96_1545;97_1844;98_2264;99_2929&stattab=map
  • Last taxonomy: Kandleria vitulina subclade
  • 16S sequence: LC064895
  • Sequence Identity:
  • Total samples: 21263
  • soil counts: 827
  • aquatic counts: 655
  • animal counts: 19322
  • plant counts: 459

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
87911Risk group (German classification)
1238791Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus vitulinus gene for 16S rRNA, partial sequence, strain: JCM 1143AB289312710ena1410657
8791Lactobacillus vitulinus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1143AB2108251450ena1410657
67770Kandleria vitulina gene for 16S ribosomal RNA, partial sequence, strain: JCM 1143LC0648951452ena1410657
67770L.vitulinus 16S ribosomal RNA small subunitM237271477ena1630

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kandleria vitulina DSM 204051410657.5wgspatric1410657
66792Kandleria vitulina DSM 204051410657.3wgspatric1410657
66792Kandleria vitulina DSM 204052660237909draftimg1410657
66792Kandleria vitulina DSM 204052561511092draftimg1410657
67770Kandleria vitulina DSM 20405GCA_000702065scaffoldncbi1410657
67770Kandleria vitulina DSM 20405GCA_001436965scaffoldncbi1410657

GC content

@refGC-contentmethod
879134.4
6777034.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes75no
motileno93.044no
gram-positiveyes95.569no
anaerobicyes97.421no
halophileno60.616no
spore-formingno85.838no
thermophileno97.468yes
glucose-utilyes87.785no
aerobicno98.834yes
flagellatedno95.221no
glucose-fermentyes76.845no

External links

@ref: 8791

culture collection no.: DSM 20405, ATCC 27783, CCUG 32236, JCM 1143, JCM 8228, LMG 18931, CIP 103154, NRRL B-14854, BCRC 14621, KCTC 3578, LMG 17754, LMG 18316, LMG 9474, NCFB 2030, NCIMB 702030, NCDO 2030

straininfo link

  • @ref: 74868
  • straininfo: 10473

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21112984Reclassification of Lactobacillus catenaformis (Eggerth 1935) Moore and Holdeman 1970 and Lactobacillus vitulinus Sharpe et al. 1973 as Eggerthia catenaformis gen. nov., comb. nov. and Kandleria vitulina gen. nov., comb. nov., respectively.Salvetti E, Felis GE, Dellaglio F, Castioni A, Torriani S, Lawson PAInt J Syst Evol Microbiol10.1099/ijs.0.029231-02010Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Lactobacillus/*classification/genetics/physiology, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidGenetics
Phylogeny30796749Intestinibaculum porci gen. nov., sp. nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.Kim JS, Choe H, Lee YR, Kim KM, Park DSJ Microbiol10.1007/s12275-019-8631-82019Animals, Bacterial Typing Techniques, Base Composition/genetics, DNA, Bacterial/genetics, Fatty Acids/analysis, Firmicutes/*classification/genetics/*isolation & purification, Intestine, Small/*microbiology, Peptidoglycan/analysis, RNA, Ribosomal, 16S/genetics, Swine/*microbiologyEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8791Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20405)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20405
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37121Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14920
50919Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32236)https://www.ccug.se/strain?id=32236
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74868Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10473.1StrainInfo: A central database for resolving microbial strain identifiers
123879Curators of the CIPCollection of Institut Pasteur (CIP 103154)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103154