Strain identifier

BacDive ID: 5352

Type strain: Yes

Species: Enterococcus thailandicus

Strain Designation: FP48-3

Strain history: <- S Tanasupawat, Chulalonkorn Univ., Thailand

NCBI tax ID(s): 417368 (species)

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General

@ref: 15934

BacDive-ID: 5352

DSM-Number: 21767

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped

description: Enterococcus thailandicus FP48-3 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from fermented sausage .

NCBI tax id

  • NCBI tax id: 417368
  • Matching level: species

strain history

@refhistory
15934<- NBRC <- S. Tanasupawat, PCU; FP48-3
67771<- S Tanasupawat, Chulalonkorn Univ., Thailand

doi: 10.13145/bacdive5352.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus thailandicus
  • full scientific name: Enterococcus thailandicus Tanasupawat et al. 2008

@ref: 15934

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus thailandicus

full scientific name: Enterococcus thailandicus Tanasupawat et al. 2008

strain designation: FP48-3

type strain: yes

Morphology

cell morphology

@refgram staincell widthcell shapemotility
32469positive0.75 µmcoccus-shapedno
67771ovoid-shapedno
67771sphere-shaped
67771positive

pigmentation

  • @ref: 32469
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 15934
  • name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/92
  • composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
15934positivegrowth30mesophilic
32469positivegrowth15-45
67771positivegrowth30mesophilic

culture pH

  • @ref: 32469
  • ability: positive
  • type: growth
  • pH: 5-9.6
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15934microaerophile
32469facultative anaerobe
67771facultative anaerobe

spore formation

@refspore formation
32469no
67771no

halophily

  • @ref: 32469
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 2-6.5 %

observation

  • @ref: 32469
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
324694853esculin+hydrolysis
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
15934++-+--++-++-+--++-++-----+--+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15934fermented sausage (mum)Khonkaen provinceThailandTHAAsia
67771From fermented sausage(mum)Khonkaen provinceThailandTHAAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_23.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_22;99_23&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: AB681572
  • Sequence Identity:
  • Total samples: 55544
  • soil counts: 1007
  • aquatic counts: 2532
  • animal counts: 51351
  • plant counts: 654

Safety information

risk assessment

  • @ref: 15934
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus hirae gene for 16S rRNA, partial sequence, strain: NRIC 0107AB3625961554ena1354
20218Enterococcus thailandicus gene for 16S rRNA, partial sequence, strain: NBRC 101867AB6815721486ena417368
15934Enterococcus thailandicus strain FP48-3 16S ribosomal RNA gene, partial sequenceEF1979941485ena417368

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus thailandicus NBRC 101867GCA_007989705contigncbi417368
66792Enterococcus thailandicus strain DSM 21767417368.6wgspatric417368
66792Enterococcus thailandicus strain NBRC 101867417368.11wgspatric417368
67771Enterococcus thailandicus DSM 21767GCA_001886265contigncbi417368

GC content

  • @ref: 15934
  • GC-content: 37.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes92.677yes
anaerobicno94.429no
halophileyes85.859no
spore-formingno90.065yes
glucose-utilyes90.198no
aerobicno95.567no
flagellatedno97.604no
thermophileno99.152yes
motileno95.765yes
glucose-fermentyes89.243no

External links

@ref: 15934

culture collection no.: DSM 21767, CCM 7793, KCTC 13134, NBRC 101867, NRIC 0107, PCU 282, TISTR 933

straininfo link

@refstraininfo
74841386903
74842410112

literature

  • topic: Phylogeny
  • Pubmed-ID: 18599707
  • title: Enterococcus thailandicus sp. nov., isolated from fermented sausage ('mum') in Thailand.
  • authors: Tanasupawat S, Sukontasing S, Lee JS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65535-0
  • year: 2008
  • mesh: Enterococcus/*classification/genetics/isolation & purification, Fermentation, *Food Microbiology, Meat Products/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity, Thailand
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
15934Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21767)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21767
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32469Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128690
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74841Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID386903.1StrainInfo: A central database for resolving microbial strain identifiers
74842Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID410112.1StrainInfo: A central database for resolving microbial strain identifiers