Strain identifier

BacDive ID: 5324

Type strain: Yes

Species: Enterococcus dispar

Strain Designation: E18-1

Strain history: CIP <- 1993, B. Phillips, AFRC, Reading, UK: strain E18-1

NCBI tax ID(s): 1139219 (strain), 44009 (species)

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General

@ref: 2636

BacDive-ID: 5324

DSM-Number: 6630

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, Gram-positive

description: Enterococcus dispar E18-1 is a microaerophile, Gram-positive bacterium that was isolated from human clinical source.

NCBI tax id

NCBI tax idMatching level
1139219strain
44009species

strain history

@refhistory
2636<- NCIMB <- M.D. Collins <- R.R. Facklam, E18-1
118944CIP <- 1993, B. Phillips, AFRC, Reading, UK: strain E18-1

doi: 10.13145/bacdive5324.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus dispar
  • full scientific name: Enterococcus dispar Collins et al. 1991

@ref: 2636

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus dispar

full scientific name: Enterococcus dispar Collins et al. 1991

strain designation: E18-1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
118944positivecoccus-shapedno
69480positive92.475

colony morphology

  • @ref: 2636
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2636COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
2636TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34408MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
118944CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
118944CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperature
2636positivegrowth37
34408positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
2636microaerophile
51449microaerophile
118944facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 91

murein

  • @ref: 2636
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate+builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118944nitrate-reduction17632
118944nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
11894435581indoleno
6838115688acetoinyes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
118944oxidase-
118944catalase-1.11.1.6
118944urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118944--++-+-+-++++---+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118944----+----++++-------+++++++++++/-+--+/----++/--+-----++/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
2636++--+-+--++-----+-+------+--++--
2636+++-+++--++-----+-++++---++/--+++/--

Isolation, sampling and environmental information

isolation

@refsample type
2636human clinical source
51449Synovial fluid
118944Other, Synovial fluid

isolation source categories

Cat1Cat2Cat3
#Infection#Medical environment#Clinic
#Host#Human

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
26362Risk group (German classification)
1189442Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus dispar 16S rRNA geneY183581397nuccore1139219
2636Enterococcus dispar 16S ribosomal RNA gene, partial sequenceAF0610071514nuccore1139219

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus dispar ATCC 51266GCA_000406945scaffoldncbi1139219
66792Enterococcus dispar ATCC 51266GCA_000407585contigncbi1139219
66792Enterococcus dispar DSM 6630GCA_001886105contigncbi44009
66792Enterococcus dispar ATCC 51266 [PRJNA206267]1139219.3wgspatric1139219
66792Enterococcus dispar ATCC 51266 [PRJNA206362]1139219.4wgspatric1139219
66792Enterococcus dispar strain DSM 663044009.3wgspatric44009
66792Enterococcus dispar ATCC 512662541047447draftimg1139219
66792Enterococcus dispar ATCC 512662545824666draftimg1139219

GC content

  • @ref: 2636
  • GC-content: 39.0
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno91no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes92.475no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no89.822yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no95.889yes
69480spore-formingspore-formingAbility to form endo- or exosporesno81.463no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.977yes
69480flagellatedmotile2+Ability to perform flagellated movementno88.479no

External links

@ref: 2636

culture collection no.: DSM 6630, ATCC 51266, NCFB 2821, NCIMB 13000, CCUG 33309, CIP 103646, CCM 4282

straininfo link

  • @ref: 74813
  • straininfo: 125770

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1370024Enterococcus dispar sp. nov. a new Enterococcus species from human sources.Collins MD, Rodrigues UM, Pigott NE, Facklam RRLett Appl Microbiol10.1111/j.1472-765x.1991.tb00514.x1991Base Sequence, Humans, Molecular Sequence Data, RNA, Bacterial/*chemistry, RNA, Ribosomal, 16S/*chemistry, Streptococcus/*classification/genetics, Transcription, GeneticPathogenicity
Phylogeny27374383Enterococcus saigonensis sp. nov., isolated from retail chicken meat and liver.Harada T, Dang VC, Nguyen DP, Nguyen TAD, Sakamoto M, Ohkuma M, Motooka D, Nakamura S, Uchida K, Jinnai M, Yonogi S, Kawahara R, Kanki M, Kawai T, Kumeda Y, Yamamoto YInt J Syst Evol Microbiol10.1099/ijsem.0.0012642016Animals, Bacterial Typing Techniques, Base Composition, Chickens/*microbiology, DNA, Bacterial/genetics, DNA-Directed RNA Polymerases/genetics, Enterococcus/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Liver/*microbiology, Meat/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, VietnamTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2636Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6630)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6630
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34408Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15466
51449Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33309)https://www.ccug.se/strain?id=33309
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74813Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125770.1StrainInfo: A central database for resolving microbial strain identifiers
118944Curators of the CIPCollection of Institut Pasteur (CIP 103646)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103646