Strain identifier

BacDive ID: 5318

Type strain: Yes

Species: Enterococcus mundtii

Strain Designation: W 102

Strain history: CIP <- 1987, NCFB <- 1981, D. Jones: strain W 102 <- J.O. Mundt: strain MUTK 559 <- W. Chesbro

NCBI tax ID(s): 53346 (species)

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General

@ref: 1844

BacDive-ID: 5318

DSM-Number: 4838

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, spore-forming, mesophilic, human pathogen

description: Enterococcus mundtii W 102 is a microaerophile, spore-forming, mesophilic human pathogen that was isolated from soil.

NCBI tax id

  • NCBI tax id: 53346
  • Matching level: species

strain history

@refhistory
1844<- NCDO <- J.O. Mundt, MUTK 559 <- W. Chesbro
67770ATCC 43186 <-- NCDO 2375 <-- D. Jones W102 <-- J. O. Mundt.
121183CIP <- 1987, NCFB <- 1981, D. Jones: strain W 102 <- J.O. Mundt: strain MUTK 559 <- W. Chesbro

doi: 10.13145/bacdive5318.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus mundtii
  • full scientific name: Enterococcus mundtii Collins et al. 1986

@ref: 1844

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus mundtii

full scientific name: Enterococcus mundtii Collins et al. 1986

strain designation: W 102

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.38
69480100positive
121183nopositivecoccus-shaped

colony morphology

@reftype of hemolysisincubation period
1844gamma1-2 days
121183

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1844COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1844TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40504MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121183CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
1844positivegrowth37mesophilic
40504positivegrowth37
46853positivegrowth37
67770positivegrowth37mesophilic
121183positivegrowth10-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
1844microaerophile
46853aerobe
121183facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480no99.911

halophily

  • @ref: 121183
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 1844
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
121183esculin+hydrolysis4853
121183nitrate-reduction17632
121183nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose+builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin+builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12118315688acetoin+

enzymes

@refvalueactivityec
1844catalase-1.11.1.6
1844cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
121183oxidase-
121183beta-galactosidase+3.2.1.23
121183alcohol dehydrogenase+1.1.1.1
121183catalase-1.11.1.6
121183gamma-glutamyltransferase-2.3.2.2
121183lysine decarboxylase-4.1.1.18
121183ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121183--++-+----++++--+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121183+--+++---++++-+--+++/--+++++++++++--++--++-+------+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
1844+++-+-++-++-++-++-++++---+--+++-
1844+++/--+-++-++-++-++-+++----+--+++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
1844soilUSAUSANorth America
46853Soil
67770Soil
121183Environment, SoilUnited KingdomGBREuropeLeicester

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
1844yes, in single cases1Risk group (German classification)
1211832Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus mundtii strain ATCC 43186 clone 1 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204542230ena53346
20218Enterococcus mundtii strain ATCC 43186 16S ribosomal RNA gene, partial sequenceKC510238815ena53346
1844Enterococcus mundtii 16S ribosomal RNA gene, partial sequenceAF0610131529ena53346
67770Enterococcus mundtii 16S rRNA gene, strain LMG 10748AJ3018361864ena53346
67770Enterococcus mundtii 16S rRNA gene, strain CECT972TAJ4208061520ena53346
67770Enterococcus mundtii gene for 16S ribosomal RNA, partial sequence, strain: JCM 8731LC0970691499ena53346
67770Enterococcus mundtii 16S rRNA geneY183401447ena53346

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus mundtii DSM 4838GCA_002813755completencbi53346
66792Enterococcus mundtii NBRC 100490GCA_007990125contigncbi53346
66792Enterococcus mundtii strain DSM 483853346.38plasmidpatric53346
66792Enterococcus mundtii strain DSM 483853346.33wgspatric53346
66792Enterococcus mundtii strain NBRC 10049053346.54wgspatric53346
66792Enterococcus mundtii DSM 4838GCA_001886035scaffoldncbi53346

GC content

@refGC-contentmethod
184438.0
6777038thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno98.154no
gram-positiveyes94.335no
anaerobicno94.973yes
halophileyes86.953no
spore-formingno89.005no
thermophileno99.69yes
glucose-utilyes89.207no
aerobicno95.542no
motileno94.931no
glucose-fermentyes88.492no

External links

@ref: 1844

culture collection no.: DSM 4838, ATCC 43186, NCDO 2375, MUTK 559, CCUG 18656, JCM 8731, BCRC 17141, BCRC 17356, CCM 4058, CECT 972, CIP 103010, HAMBI 1570, KCTC 3630, LMG 10748, NBRC 100490, NCIMB 13132, NCIMB 702375, NCTC 12363

straininfo link

  • @ref: 74807
  • straininfo: 2266

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7960141Resistance of Enterococcus faecium to neutrophil-mediated phagocytosis.Arduino RC, Jacques-Palaz K, Murray BE, Rakita RMInfect Immun10.1128/iai.62.12.5587-5594.19941994Bacterial Capsules/*chemistry, Enterococcus faecium/classification/*immunology/pathogenicity/ultrastructure, Humans, Microscopy, Electron, Microscopy, Fluorescence, N-Acetylneuraminic Acid, Neutrophils/*immunology/ultrastructure, Opsonin Proteins, *Phagocytosis, Sialic Acids/analysis, Species Specificity, VirulencePathogenicity
Pathogenicity10030000Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution.Bahirathan M, Puente L, Seyfried PCan J Microbiol10.1139/cjm-44-11-10661998Animals, Anti-Bacterial Agents/pharmacology, Dogs, Enterococcus/*classification/drug effects/*isolation & purification, *Environmental Microbiology, Feces/*microbiology, Humans, Microbial Sensitivity Tests, Pigments, Biological, Prohibitins, Restriction Mapping, Water Microbiology, *Water PollutionPhylogeny
Phylogeny30203322Genetic diversity and antimicrobial activity of lactic acid bacteria in the preparation of traditional fermented potato product 'tunta'.Ramos ER, Santos RA, Velazquez E, Velezmoro CE, Zuniga DEWorld J Microbiol Biotechnol10.1007/s11274-018-2525-52018Anti-Infective Agents/*metabolism/pharmacology, Biodiversity, Colony Count, Microbial, DNA, Bacterial/analysis, Fermentation, Fermented Foods/*microbiology, Food Microbiology, Genes, Bacterial/genetics, *Genetic Variation, Lactobacillales/classification/*genetics/isolation & purification/*metabolism, Listeria/drug effects, Microbial Consortia/genetics/physiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Solanum tuberosum/*microbiology, Yeasts/genetics/isolation & purification/physiologyMetabolism
33964599Efficient conversion of hemicellulose sugars from spent sulfite liquor into optically pure L-lactic acid by Enterococcus mundtii.Hoheneder R, Fitz E, Bischof RH, Russmayer H, Ferrero P, Peacock S, Sauer MBioresour Technol10.1016/j.biortech.2021.1252152021Enterococcus, Fermentation, *Lactic Acid, Polysaccharides, *Sugars, Sulfites
Phylogeny36496711Investigating the Probiotic Properties and Antimicrobial Activity of Lactic Acid Bacteria Isolated from an Iranian Fermented Dairy Product, Kashk.Saboori B, Shahidi F, Hedayati S, Javadmanesh AFoods10.3390/foods112339042022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1844Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4838)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4838
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40504Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14760
46853Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18656)https://www.ccug.se/strain?id=18656
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74807Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2266.1StrainInfo: A central database for resolving microbial strain identifiers
121183Curators of the CIPCollection of Institut Pasteur (CIP 103010)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103010