Strain identifier

BacDive ID: 5298

Type strain: No

Species: Enterococcus faecium

Strain Designation: 64/3

Strain history: <- G. Reuter; 64/3 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin

NCBI tax ID(s): 1352 (species)

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General

@ref: 5097

BacDive-ID: 5298

DSM-Number: 13589

keywords: genome sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus faecium 64/3 is a microaerophile, mesophilic bacterium that was isolated from human faeces.

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history

  • @ref: 5097
  • history: <- G. Reuter; 64/3 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin

doi: 10.13145/bacdive5298.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 5097

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

strain designation: 64/3

type strain: no

Morphology

colony morphology

  • @ref: 5097
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5097COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5097TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 5097
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5097
  • oxygen tolerance: microaerophile

observation

  • @ref: 5097
  • observation: Vancomycin sensitive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
5097catalase-1.11.1.6
5097cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5097+++---++-++-++-++-++-+---+--+/--+-

Isolation, sampling and environmental information

isolation

  • @ref: 5097
  • sample type: human faeces
  • geographic location: Berlin
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 5097
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus faecium 64/3GCA_001298485completencbi1352
66792Enterococcus faecium 64/32671181005completeimg1352

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.93no
flagellatedno98.062no
gram-positiveyes94.341no
anaerobicno96.743yes
aerobicno93.996no
halophileyes92.755no
spore-formingno91.253no
glucose-utilyes89.016no
thermophileno99.722yes
glucose-fermentyes87.572no

External links

@ref: 5097

culture collection no.: DSM 13589

straininfo link

  • @ref: 74788
  • straininfo: 143342

Reference

@idauthorscataloguedoi/urltitle
5097Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13589)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13589
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR
74788Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID143342.1StrainInfo: A central database for resolving microbial strain identifiers