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Strain identifier

BacDive ID: 5298

Type strain: No

Species: Enterococcus faecium

Strain Designation: 64/3

Strain history: <- G. Reuter; 64/3 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin

NCBI tax ID(s): 1352 (species)

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General

@ref: 5097

BacDive-ID: 5298

DSM-Number: 13589

keywords: genome sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus faecium 64/3 is a microaerophile, mesophilic bacterium that was isolated from human faeces.

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history: <- G. Reuter; 64/3 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin

doi: 10.13145/bacdive5298.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 5097

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

strain designation: 64/3

type strain: no

Morphology

colony morphology

  • @ref: 5097
  • type of hemolysis: alpha
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5097COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5097TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 5097
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5097
  • oxygen tolerance: microaerophile

observation

  • @ref: 5097
  • observation: Vancomycin sensitive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838116199urea-hydrolysis
6838116443D-tagatose-builds acid from
683816731melezitose-builds acid from
6838128053melibiose-builds acid from
6838117306maltose+builds acid from
6838127941pullulan-builds acid from
6838128087glycogen-builds acid from
68381606565hippurate-hydrolysis
6838140585alpha-cyclodextrin+builds acid from
6838118333D-arabitol-builds acid from
6838130849L-arabinose+builds acid from
6838117992sucrose+builds acid from
6838116634raffinose-builds acid from
6838127082trehalose+builds acid from
6838117716lactose+builds acid from
6838130911sorbitol-builds acid from
6838116899D-mannitol+builds acid from
6838116988D-ribose+builds acid from
6838129016arginine+hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
5097catalase-1.11.1.6
5097cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5097+++---++-++-++-++-++-+---+--+/--+-

Isolation, sampling and environmental information

isolation

  • @ref: 5097
  • sample type: human faeces
  • geographic location: Berlin
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 5097
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus faecium 64/3GCA_001298485completencbi1352
66792Enterococcus faecium 64/32671181005completeimg1352

External links

@ref: 5097

culture collection no.: DSM 13589

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/401037

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5097Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13589)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13589
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68381Automatically annotated from API rID32STR