Strain identifier

BacDive ID: 5273

Type strain: No

Species: Enterococcus casseliflavus

Strain Designation: CA 3

Strain history: <- CCM <- R. Pompei; CA 3 (Enterococcus flavescens)

NCBI tax ID(s): 37734 (species)

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General

@ref: 3127

BacDive-ID: 5273

DSM-Number: 7371

keywords: Bacteria, microaerophile, mesophilic

description: Enterococcus casseliflavus CA 3 is a microaerophile, mesophilic bacterium that was isolated from human abscess.

NCBI tax id

  • NCBI tax id: 37734
  • Matching level: species

strain history

  • @ref: 3127
  • history: <- CCM <- R. Pompei; CA 3 (Enterococcus flavescens)

doi: 10.13145/bacdive5273.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus casseliflavus
  • full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984
  • synonyms

    @refsynonym
    20215Streptococcus faecium subsp. casseliflavus
    20215Enterococcus flavescens
    20215Streptococcus casseliflavus

@ref: 3127

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus casseliflavus

full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984

strain designation: CA 3

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3127COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3127TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 3127
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3127
  • oxygen tolerance: microaerophile

murein

  • @ref: 3127
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine+hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose+builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
3127+++-+--+-+++++-+/-+-++++---+++/-+-+-
50156+++-+--+-+++++-++-++++---++-+-+-

Isolation, sampling and environmental information

isolation

@refsample type
3127human abscess
50156Human abscess

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Other#Abscess
#Infection#Patient

Safety information

risk assessment

  • @ref: 3127
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

GC content

  • @ref: 3127
  • GC-content: 42.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 3127

culture collection no.: CCUG 30568, LMG 16313, DSM 7371, CCM 4240

straininfo link

  • @ref: 74763
  • straininfo: 8811

Reference

@idauthorscataloguedoi/urltitle
3127Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7371)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7371
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
50156Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30568)https://www.ccug.se/strain?id=30568
68381Automatically annotated from API rID32STR
74763Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8811.1StrainInfo: A central database for resolving microbial strain identifiers