Strain identifier

BacDive ID: 5184

Type strain: Yes

Species: Xenorhabdus nematophila subsp. bovienii

Strain Designation: T 228, T228

Strain history: CIP <- 2006, P. Tailliez, Montpellier, France: strain T228 <- N. Boemare <- R. J. Akhurst

NCBI tax ID(s): 40576 (species)

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General

@ref: 1761

BacDive-ID: 5184

DSM-Number: 4766

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, animal pathogen

description: Xenorhabdus nematophila subsp. bovienii T 228 is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from nematode Steinernema bibionis.

NCBI tax id

  • NCBI tax id: 40576
  • Matching level: species

strain history

@refhistory
1761<- UQM
116906CIP <- 2006, P. Tailliez, Montpellier, France: strain T228 <- N. Boemare <- R. J. Akhurst

doi: 10.13145/bacdive5184.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Morganellaceae
  • genus: Xenorhabdus
  • species: Xenorhabdus nematophila subsp. bovienii
  • full scientific name: Xenorhabdus nematophila subsp. bovienii corrig. Akhurst 1983

  • synonyms

    @refsynonym
    20215Xenorhabdus nematophilus subsp. bovienii
    20215Xenorhabdus nematophilus subsp. bovenii
    20215Xenorhabdus bovienii

@ref: 1761

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Morganellaceae

genus: Xenorhabdus

species: Xenorhabdus bovienii

full scientific name: Xenorhabdus bovienii (Akhurst 1983) Akhurst and Boemare 1993

strain designation: T 228, T228

type strain: yes

Morphology

cell morphology

  • @ref: 116906
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 116906

multimedia

  • @ref: 1761
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_4766.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1761TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
1761XENORHABDUS MEDIUM (DSMZ Medium 423)yeshttps://mediadive.dsmz.de/medium/423Name: XENORHABDUS MEDIUM (DSMZ Medium 423) Composition: Agar 15.0 g/l Peptone 10.0 g/l NaCl 5.0 g/l Yeast extract 5.0 g/l Distilled water
40128MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116906CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
1761positivegrowth28mesophilic
40128positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116906
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68369malate-assimilation25115
68369adipate-assimilation17128
68369decanoate-assimilation27689
68369gluconate+assimilation24265
68369maltose+assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol-assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose-assimilation30849
68369D-glucose+assimilation17634
68369gelatin-hydrolysis5291
68369esculin-hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369tryptophan-energy source27897
68369nitrate-reduction17632
116906nitrate-reduction17632
116906nitrite-reduction16301

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11690635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
116906oxidase-
116906alcohol dehydrogenase-1.1.1.1
116906catalase-1.11.1.6
116906lysine decarboxylase-4.1.1.18
116906ornithine decarboxylase-4.1.1.17
116906urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116906-+++-++-+-++-----+--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
1761--+/------+-+-+++-----+/-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116906+---------+++--------+-----+---+-----------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
1761nematode Steinernema bibionisSteinernema bibionisTasmaniaAustraliaAUSAustralia and Oceania
116906Animal, Nematode, Steinernema bibionisDover, TasmaniaAustraliaAUSAustralia and Oceania1983

isolation source categories

  • Cat1: #Host
  • Cat2: #Invertebrates (Other)
  • Cat3: #Nematoda

taxonmaps

  • @ref: 69479
  • File name: preview.99_1824.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_27;97_1204;98_1443;99_1824&stattab=map
  • Last taxonomy: Xenorhabdus bovienii subclade
  • 16S sequence: X82252
  • Sequence Identity:
  • Total samples: 440
  • soil counts: 300
  • aquatic counts: 13
  • animal counts: 110
  • plant counts: 17

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
1761yes1Risk group (German classification)
1169061Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Xenorhabdus bovienii gene for 16S rRNA, partial sequence, strain: ATCC 35271D780071481ena40576
20218Xenorhabdus bovienii 16S ribosomal RNA gene, partial sequenceAY2786731497ena40576
20218X.bovienii 16S rRNA gene (DSM 4766)X822521497ena40576

External links

@ref: 1761

culture collection no.: DSM 4766, ATCC 35271, CCM 7080, UQM 2210, CIP 109144

straininfo link

  • @ref: 74676
  • straininfo: 11106

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1761Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4766)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4766
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40128Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6853
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74676Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID11106.1StrainInfo: A central database for resolving microbial strain identifiers
116906Curators of the CIPCollection of Institut Pasteur (CIP 109144)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109144