Strain identifier
BacDive ID: 5170
Type strain: 
Species: Serratia symbiotica
Strain Designation: CWBI-2.3
Strain history: CIP <- 2016, DSMZ <- A. Sabri, Centre Wallon Biol. Indust., Liege Univ., Liege, Belgium: strain CWBI-2.3
NCBI tax ID(s): 138074 (species)
version 8.1 (current version)
General
@ref: 16770
BacDive-ID: 5170
DSM-Number: 23270
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped
description: Serratia symbiotica CWBI-2.3 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from black bean aphid Aphis fabae.
NCBI tax id
- NCBI tax id: 138074
- Matching level: species
strain history
| @ref | history |
|---|---|
| 16770 | <- A. Sabri, Centre Wallon de Biol. Industrielle, Univ. Liège, Belgium; CWBI-2.3 |
| 123398 | CIP <- 2016, DSMZ <- A. Sabri, Centre Wallon Biol. Indust., Liege Univ., Liege, Belgium: strain CWBI-2.3 |
doi: 10.13145/bacdive5170.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Yersiniaceae
- genus: Serratia
- species: Serratia symbiotica
- full scientific name: Serratia symbiotica Sabri et al. 2011
synonyms
- @ref: 20215
- synonym: Serratia symbiotica
@ref: 16770
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Yersiniaceae
genus: Serratia
species: Serratia symbiotica
full scientific name: Serratia symbiotica Sabri et al. 2011
strain designation: CWBI-2.3
type strain: yes
Morphology
cell morphology
- @ref: 123398
- gram stain: negative
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 16770 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
| 41623 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
| 123398 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16770 | positive | growth | 28 | mesophilic |
| 41623 | positive | growth | 30 | mesophilic |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | country | origin.country | continent |
|---|---|---|---|---|---|
| 16770 | black bean aphid Aphis fabae | Aphis fabae | Belgium | BEL | Europe |
| 123398 | Black bean aphid, Aphis fabae | Belgium | BEL | Europe |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
taxonmaps
- @ref: 69479
- File name: preview.99_2471.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_1333;97_1583;98_1925;99_2471&stattab=map
- Last taxonomy: Yersiniaceae
- 16S sequence: GU394001
- Sequence Identity:
- Total samples: 10317
- soil counts: 447
- aquatic counts: 1079
- animal counts: 8118
- plant counts: 673
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 16770 | 1 | Risk group (German classification) |
| 123398 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 16770
- description: Serratia symbiotica strain CWBI-2.3 16S ribosomal RNA gene, partial sequence
- accession: GU394001
- length: 1478
- database: ena
- NCBI tax ID: 138074
Genome sequences
- @ref: 66792
- description: Serratia symbiotica CWBI-2.3
- accession: GCA_000821185
- assembly level: complete
- database: ncbi
- NCBI tax ID: 138074
GC content
- @ref: 16770
- GC-content: 52.7
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 64.864 | no |
| motile | yes | 64.864 | no |
| flagellated | no | 88.159 | no |
| flagellated | no | 88.159 | no |
| gram-positive | no | 97.984 | no |
| gram-positive | no | 97.984 | no |
| anaerobic | no | 98.105 | no |
| anaerobic | no | 98.105 | no |
| aerobic | no | 63.403 | no |
| aerobic | no | 63.403 | no |
| halophile | no | 79.124 | no |
| halophile | no | 79.124 | no |
| spore-forming | no | 96.947 | no |
| spore-forming | no | 96.947 | no |
| glucose-util | yes | 85.244 | no |
| glucose-util | yes | 85.244 | no |
| thermophile | no | 98.043 | yes |
| thermophile | no | 98.043 | yes |
| glucose-ferment | yes | 79.278 | no |
| glucose-ferment | yes | 79.278 | no |
External links
@ref: 16770
culture collection no.: DSM 23270, CIP 111023, LMG 25624
straininfo link
- @ref: 74662
- straininfo: 369499
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 20870890 | Isolation, pure culture and characterization of Serratia symbiotica sp. nov., the R-type of secondary endosymbiont of the black bean aphid Aphis fabae. | Sabri A, Leroy P, Haubruge E, Hance T, Frere I, Destain J, Thonart P | Int J Syst Evol Microbiol | 10.1099/ijs.0.024133-0 | 2010 | Aerobiosis, Animals, Aphids/*microbiology, Bacterial Typing Techniques, Cluster Analysis, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Serratia/*classification/genetics/*isolation & purification/physiology, Symbiosis | Genetics |
| Genetics | 25146134 | Whole-Genome Sequence of Serratia symbiotica Strain CWBI-2.3T, a Free-Living Symbiont of the Black Bean Aphid Aphis fabae. | Foray V, Grigorescu AS, Sabri A, Haubruge E, Lognay G, Francis F, Fauconnier ML, Hance T, Thonart P | Genome Announc | 10.1128/genomeA.00767-14 | 2014 | ||
| Pathogenicity | 25811863 | Infection dynamic of symbiotic bacteria in the pea aphid Acyrthosiphon pisum gut and host immune response at the early steps in the infection process. | Renoz F, Noel C, Errachid A, Foray V, Hance T | PLoS One | 10.1371/journal.pone.0122099 | 2015 | Animals, Aphids/genetics/*immunology/*microbiology, Bacteria/genetics/*immunology, Gastrointestinal Microbiome/*immunology, Gene Expression Profiling, Genes, Insect, Host-Pathogen Interactions/*immunology, Symbiosis, Time Factors | |
| Proteome | 28503376 | Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont. | Renoz F, Champagne A, Degand H, Faber AM, Morsomme P, Foray V, Hance T | PeerJ | 10.7717/peerj.3291 | 2017 | ||
| Biotechnology | 33277267 | Engineering a Culturable Serratia symbiotica Strain for Aphid Paratransgenesis. | Elston KM, Perreau J, Maeda GP, Moran NA, Barrick JE | Appl Environ Microbiol | 10.1128/AEM.02245-20 | 2020 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 16770 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23270) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23270 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 41623 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/34635 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 74662 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID369499.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
| 123398 | Curators of the CIP | Collection of Institut Pasteur (CIP 111023) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111023 |