Strain identifier
BacDive ID: 5003
Type strain: 
Species: Pantoea cypripedii
Strain history: <- DSM 60216 <- L.E. Webb <- R. Marwitz
NCBI tax ID(s): 55209 (species)
version 8.1 (current version)
General
@ref: 9185
BacDive-ID: 5003
DSM-Number: 30182
keywords: Bacteria, mesophilic, plant pathogen
description: Pantoea cypripedii DSM 30182 is a mesophilic plant pathogen that was isolated from Paphiopedilum philippinense, soft rot of leaf.
NCBI tax id
- NCBI tax id: 55209
- Matching level: species
strain history
- @ref: 9185
- history: <- DSM 60216 <- L.E. Webb <- R. Marwitz
doi: 10.13145/bacdive5003.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Erwiniaceae
- genus: Pantoea
- species: Pantoea cypripedii
- full scientific name: Pantoea cypripedii (Hori 1911) Brady et al. 2010
synonyms
@ref synonym 20215 Bacillus cypripedii 20215 Pectobacterium cypripedii 20215 Erwinia cypripedii
@ref: 9185
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Erwiniaceae
genus: Pantoea
species: Pantoea cypripedii
full scientific name: Pantoea cypripedii (Hori 1911) Brady et al. 2010
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9185 | GLUCOSE YEAST EXTRACT MEDIUM (DSMZ Medium 54) | yes | https://mediadive.dsmz.de/medium/54 | Name: GLUCOSE YEAST EXTRACT MEDIUM (DSMZ Medium 54) Composition: CaCO3 20.0 g/l Glucose 20.0 g/l Agar 17.0 g/l Yeast extract 10.0 g/l Distilled water |
| 9185 | REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) | yes | https://mediadive.dsmz.de/medium/1a | Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
| 9185 | YPGA (DSMZ Medium 1015) | yes | https://mediadive.dsmz.de/medium/1015 | Name: YPGA (DSMZ Medium 1015) Composition: Agar 15.0 g/l Glucose 7.0 g/l Bacto peptone 7.0 g/l Yeast extract 7.0 g/l Distilled water |
culture temp
- @ref: 9185
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 17632 | nitrate | - | reduction |
| 68368 | 30849 | L-arabinose | + | fermentation |
| 68368 | 27613 | amygdalin | + | fermentation |
| 68368 | 28053 | melibiose | + | fermentation |
| 68368 | 17992 | sucrose | + | fermentation |
| 68368 | 62345 | L-rhamnose | + | fermentation |
| 68368 | 30911 | sorbitol | - | fermentation |
| 68368 | 17268 | myo-inositol | + | fermentation |
| 68368 | 16899 | D-mannitol | + | fermentation |
| 68368 | 17634 | D-glucose | + | fermentation |
| 68368 | 5291 | gelatin | - | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | - | hydrolysis |
| 68368 | 16947 | citrate | + | assimilation |
| 68368 | 18257 | ornithine | - | degradation |
| 68368 | 25094 | lysine | - | degradation |
| 68368 | 29016 | arginine | - | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 17997 | dinitrogen | no |
| 68368 | 16301 | nitrite | no |
| 68368 | 15688 | acetoin | no |
| 68368 | 35581 | indole | no |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | - | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | cytochrome oxidase | - | 1.9.3.1 |
| 68368 | gelatinase | - | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | - | 3.5.1.5 |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 |
| 68368 | lysine decarboxylase | - | 4.1.1.18 |
| 68368 | arginine dihydrolase | - | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX | NO2 | N2 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 9185 | + | - | - | - | + | - | - | - | - | - | - | + | + | + | - | + | + | + | + | + | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 9185
- sample type: Paphiopedilum philippinense, soft rot of leaf
- host species: Paphiopedilum philippinense
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Decomposing plant |
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Leaf (Phyllosphere) |
Safety information
risk assessment
- @ref: 9185
- pathogenicity plant: yes
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 9185
culture collection no.: DSM 30182, DSM 60216
straininfo link
- @ref: 74498
- straininfo: 2755
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 9185 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30182) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-30182 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68368 | Automatically annotated from API 20E | |||
| 74498 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID2755.1 | StrainInfo: A central database for resolving microbial strain identifiers |