Strain identifier

BacDive ID: 4960

Type strain: No

Species: Klebsiella pneumoniae subsp. pneumoniae

Strain history: CIP <- 1994, ATCC <- E.R. Squibb & Sons: strain NCDC 400-68

NCBI tax ID(s): 72407 (subspecies)

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General

@ref: 9135

BacDive-ID: 4960

DSM-Number: 30105

keywords: Bacteria, facultative anaerobe, Gram-negative, rod-shaped, human pathogen

description: Klebsiella pneumoniae subsp. pneumoniae DSM 30105 is a facultative anaerobe, Gram-negative, rod-shaped human pathogen of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 72407
  • Matching level: subspecies

strain history

@refhistory
9135<- ATCC <- E.R. Squibb & Sons Co.
121884CIP <- 1994, ATCC <- E.R. Squibb & Sons: strain NCDC 400-68

doi: 10.13145/bacdive4960.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Klebsiella
  • species: Klebsiella pneumoniae subsp. pneumoniae
  • full scientific name: Klebsiella pneumoniae subsp. pneumoniae (Schroeter 1886) Ørskov 1984

@ref: 9135

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Klebsiella

species: Klebsiella pneumoniae subsp. pneumoniae

full scientific name: Klebsiella pneumoniae subsp. pneumoniae (Schroeter 1886) Ørskov 1984

type strain: no

Morphology

cell morphology

  • @ref: 121884
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9135NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37040MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
121884CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
9135positivegrowth37
37040positivegrowth30
121884positivegrowth10-41
121884nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 121884
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose+builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen+builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol+builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose+fermentation30849
68368amygdalin+fermentation27613
68368melibiose+fermentation28053
68368sucrose+fermentation17992
68368L-rhamnose+fermentation62345
68368sorbitol+fermentation30911
68368myo-inositol+fermentation17268
68368D-mannitol+fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan-energy source27897
68368ornithine-degradation18257
68368lysine+degradation25094
68368arginine-hydrolysis29016
121884mannitol+fermentation29864
121884citrate+carbon source16947
121884glucose+fermentation17234
121884lactose+fermentation17716
121884nitrate+reduction17632
121884nitrite-reduction16301
121884malonate-assimilation15792
121884sodium thiosulfate-builds gas from132112
121884glucose+degradation17234

metabolite production

@refChebi-IDmetaboliteproduction
6836835581indoleno
6836816136hydrogen sulfideno
12188435581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836835581indole-
12188415688acetoin+
12188417234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
121884oxidase-
121884beta-galactosidase+3.2.1.23
121884alcohol dehydrogenase-1.1.1.1
121884gelatinase-
121884catalase+1.11.1.6
121884lysine decarboxylase+4.1.1.18
121884ornithine decarboxylase-4.1.1.17
121884phenylalanine ammonia-lyase-4.3.1.24
121884tryptophan deaminase-
121884urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121884-+---+----++++-++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
9135+-+-+----+-+++++++++-
9135+-+-+----+-+++++++++-
9135+-+-+----+-+++++++++-
9135+-+---+----+++++++++-
9135--+-+-+--+-+++++++++-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121884+--+++-+-++++++-+++-++-+++++++++--+++-+----++-++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121884+++++++++++++++++++++++++-+----++++-++---+-+++--++++-++++--+++-------+---+-+--+++-++--+-+++++++----

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
9135yesyes2Risk group (German classification)
1218842Risk group (French classification)

External links

@ref: 9135

culture collection no.: DSM 30105, ATCC 9997, CIP 104034, NCDC 400-68

straininfo link

  • @ref: 74456
  • straininfo: 3115

Reference

@idauthorscataloguedoi/urltitle
9135Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 30105)https://www.dsmz.de/collection/catalogue/details/culture/DSM-30105
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37040Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15897
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
74456Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3115.1StrainInfo: A central database for resolving microbial strain identifiers
121884Curators of the CIPCollection of Institut Pasteur (CIP 104034)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104034