Strain identifier
BacDive ID: 4926
Type strain: 
Species: Escherichia coli
Strain Designation: ID 12-18
Strain history: <- N. Kunze, Universitätsmedizin Göttingen, Germany, Zentrum Anaesthesiologie, Rettungs- und Intensivmedizin, Göttingen ZARI 2012
NCBI tax ID(s): 562 (species)
version 8.1 (current version)
General
@ref: 18136
BacDive-ID: 4926
DSM-Number: 25944
keywords: Bacteria, mesophilic, antibiotic resistance, human pathogen
description: Escherichia coli ID 12-18 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from Tracheal secretion.
NCBI tax id
- NCBI tax id: 562
- Matching level: species
strain history
- @ref: 18136
- history: <- N. Kunze, Universitätsmedizin Göttingen, Germany, Zentrum Anaesthesiologie, Rettungs- und Intensivmedizin, Göttingen ZARI 2012
doi: 10.13145/bacdive4926.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Escherichia
- species: Escherichia coli
- full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus coli
@ref: 18136
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Escherichia
species: Escherichia coli
full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919
strain designation: ID 12-18
type strain: no
Culture and growth conditions
culture medium
- @ref: 18136
- name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
- growth: yes
- link: https://mediadive.dsmz.de/medium/381
- composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water
culture temp
- @ref: 18136
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
antibiogram
- @ref: 18136
- medium: Mueller-Hinton Agar
- incubation temperature: 37
- incubation time: 1
- oxygen condition: aerob
- Penicillin G: 0
- oxacillin: 0
- ampicillin: 0
- ticarcillin: 0
- mezlocillin: 0
- cefalotin: 0
- cefazolin: 0
- cefotaxime: 8
- aztreonam: 10
- imipenem: 32
- tetracycline: 28
- chloramphenicol: 30
- gentamycin: 0
- amikacin: 18
- vancomycin: 0
- erythromycin: 0
- lincomycin: 0
- ofloxacin: 0
- norfloxacin: 0
- colistin: 14
- pipemidic acid: 0
- nitrofurantoin: 22
- bacitracin: 0
- polymyxin b: 16
- kanamycin: 10
- neomycin: 20
- doxycycline: 22
- ceftriaxone: 6-8
- clindamycin: 0
- fosfomycin: 16-18
- moxifloxacin: 0
- linezolid: 0
- nystatin: 0
- quinupristin/dalfopristin: 0
- teicoplanin: 0
- piperacillin/tazobactam: 22
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 30849 | L-arabinose | + | fermentation |
| 68368 | 27613 | amygdalin | - | fermentation |
| 68368 | 17992 | sucrose | + | fermentation |
| 68368 | 62345 | L-rhamnose | + | fermentation |
| 68368 | 30911 | sorbitol | + | fermentation |
| 68368 | 17268 | myo-inositol | - | fermentation |
| 68368 | 16899 | D-mannitol | + | fermentation |
| 68368 | 17634 | D-glucose | + | fermentation |
| 68368 | 5291 | gelatin | - | hydrolysis |
| 68368 | 27897 | tryptophan | + | energy source |
| 68368 | 16199 | urea | - | hydrolysis |
| 68368 | 16947 | citrate | - | assimilation |
| 68368 | 18257 | ornithine | + | degradation |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 15688 | acetoin | no |
| 68368 | 35581 | indole | yes |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | - | |
| 68368 | 35581 | indole | + |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | cytochrome oxidase | - | 1.9.3.1 |
| 68368 | gelatinase | - | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | - | 3.5.1.5 |
| 68368 | ornithine decarboxylase | + | 4.1.1.17 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 18136 | + | - | +/- | + | - | - | - | - | + | - | - | + | + | - | + | + | + | - | - | + | |
| 18136 | + | + | + | + | - | - | - | - | + | - | - | + | + | - | + | + | + | + | - | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 18136
- sample type: Tracheal secretion
- geographic location: Göttingen
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body-Site | #Oral cavity and airways | #Trachea |
| #Host Body Product | #Fluids |
Safety information
risk assessment
- @ref: 18136
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
External links
@ref: 18136
culture collection no.: DSM 25944
straininfo link
- @ref: 74423
- straininfo: 403127
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 18136 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25944) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25944 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68368 | Automatically annotated from API 20E | |||
| 74423 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID403127.1 | StrainInfo: A central database for resolving microbial strain identifiers |