Strain identifier

BacDive ID: 4922

Type strain: No

Species: Escherichia coli

Strain Designation: BfR/NRL-AR-E467, NRL-ECRL 102/2005, NRL-Salm05E00174

Strain history: <- B. Guerra Román, Natl. Reference Lab. Antimicrobial Resistance (NRL-AR), Federal Inst. Risk Assessment (BfR), Berlin, Germany; BfR/NRL-AR-E467 <- Natl. Salmonella Reference Lab. (NRL-Salm), Federal Inst. Risk Assessment (BfR), Berlin, Germany; NRL-Salm05E00174 <- L. Beutin, Natl. Reference Lab. Escherichia coli (NRL-EC), Federal Inst. Risk Assessment (BfR), Berlin, Germany; NRL-ECRL 102/2005 <- Charité - Universitätsmedizin Berlin, Germany

NCBI tax ID(s): 562 (species)

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General

@ref: 16454

BacDive-ID: 4922

DSM-Number: 22664

keywords: Bacteria, aerobe, mesophilic, antibiotic resistance, human pathogen

description: Escherichia coli BfR/NRL-AR-E467 is an aerobe, mesophilic human pathogen that has multiple antibiotic resistances and was isolated from human clinical isolate.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 16454
  • history: <- B. Guerra Román, Natl. Reference Lab. Antimicrobial Resistance (NRL-AR), Federal Inst. Risk Assessment (BfR), Berlin, Germany; BfR/NRL-AR-E467 <- Natl. Salmonella Reference Lab. (NRL-Salm), Federal Inst. Risk Assessment (BfR), Berlin, Germany; NRL-Salm05E00174 <- L. Beutin, Natl. Reference Lab. Escherichia coli (NRL-EC), Federal Inst. Risk Assessment (BfR), Berlin, Germany; NRL-ECRL 102/2005 <- Charité - Universitätsmedizin Berlin, Germany

doi: 10.13145/bacdive4922.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 16454

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: BfR/NRL-AR-E467, NRL-ECRL 102/2005, NRL-Salm05E00174

type strain: no

Culture and growth conditions

culture medium

  • @ref: 16454
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16454positivegrowth30mesophilic
62729positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62729
  • oxygen tolerance: aerobe

antibiogram

  • @ref: 16454
  • medium: Mueller-Hinton Agar
  • Penicillin G: 0
  • oxacillin: 0
  • ampicillin: 0
  • ticarcillin: 0
  • mezlocillin: 10
  • cefalotin: 0
  • cefazolin: 8
  • cefotaxime: 18
  • aztreonam: 26
  • imipenem: 34
  • tetracycline: 8
  • chloramphenicol: 28-30
  • gentamycin: 20
  • amikacin: 18-20
  • vancomycin: 0
  • erythromycin: 0
  • lincomycin: 0
  • ofloxacin: 6-8
  • norfloxacin: 0
  • colistin: 14
  • pipemidic acid: 0
  • nitrofurantoin: 26
  • bacitracin: 0
  • polymyxin b: 14
  • kanamycin: 24
  • neomycin: 18
  • doxycycline: 10
  • ceftriaxone: 20
  • clindamycin: 0
  • fosfomycin: 34
  • moxifloxacin: 0
  • linezolid: 0
  • nystatin: 0
  • quinupristin/dalfopristin: 0
  • teicoplanin: 0
  • piperacillin/tazobactam: 20

compound production

  • @ref: 16454
  • compound: CTX-M15 beta lactamase

observation

  • @ref: 16454
  • observation: ESBL

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
6837418257ornithine+degradation
6837429016arginine-hydrolysis
6837425094lysine+degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid+builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol+builds acid from
6837417306maltose+builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan+energy source
6837417634D-glucose+builds acid from
6837417992sucrose+builds acid from
6837430849L-arabinose+builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose+builds acid from
6837462345L-rhamnose+builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6837435581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837435581indole+
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase+3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase+3.2.1.23
68374N-acetyl-beta-glucosaminidase-3.2.1.52
68374beta-glucuronidase+3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase+4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase+4.1.1.17
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
16454+-++----+--++-++++-+-

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
62729+-+--+--+-++--+-+-++++--+++-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16454human clinical isolateBerlinGermanyDEUEurope
62729Human clinical isolateBerlinGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Infection#Patient
#Infection#Medical environment#Clinic
#Infection#Patient#Specimen

Safety information

risk assessment

  • @ref: 16454
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 16454

culture collection no.: DSM 22664, CCUG 61908

straininfo link

  • @ref: 74420
  • straininfo: 396568

Reference

@idauthorscataloguedoi/urltitle
16454Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22664)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22664
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
62729Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61908)https://www.ccug.se/strain?id=61908
68368Automatically annotated from API 20E
68374Automatically annotated from API ID32E
74420Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID396568.1StrainInfo: A central database for resolving microbial strain identifiers