Strain identifier

BacDive ID: 4626

Type strain: No

Species: Escherichia coli

Strain Designation: K12 NAR 30, NAR 30

Strain history: <- D.P. Clark, Carbondale, Illinois

NCBI tax ID(s): 562 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2788

BacDive-ID: 4626

DSM-Number: 6722

keywords: mesophilic, Bacteria

description: Escherichia coli K12 NAR 30 is a mesophilic bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history: <- D.P. Clark, Carbondale, Illinois

doi: 10.13145/bacdive4626.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 2788

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: K12 NAR 30, NAR 30

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2788MINERAL MEDIUM M9 for E. coli JM strains (DSMZ Medium 382)yeshttps://bacmedia.dsmz.de/medium/382Name: MINERAL MEDIUM M9 for E. coli JM strains (DSMZ Medium 382) Composition: Na2HPO4 6.0 g/l KH2PO4 3.0 g/l Glucose 2.0 g/l NH4Cl 1.0 g/l NaCl 0.5 g/l Thiamine-HCl x 2 H2O 0.373301 g/l MgSO4 0.12037 g/l Proline 0.02 g/l CaCl2 0.011098 g/l Distilled water
2788LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://bacmedia.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 2788
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836830911sorbitol+fermentation
6836816899D-mannitol+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole+
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
2788+-+-----+--++-++---+
2788+-+-----+---++++---+
2788+-+-----+--++-++---+-
2788+-------+--++-+----+
2788+-+-----+--++-++---+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
2788+++----+++++++++-+-+

Safety information

risk assessment

  • @ref: 2788
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 2788

culture collection no.: DSM 6722

straininfo link

@refpassport
20218http://www.straininfo.net/strains/160262
20218http://www.straininfo.net/strains/160264

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2788Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6722)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6722
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE