Strain identifier

BacDive ID: 4626

Type strain: No

Species: Escherichia coli

Strain Designation: K12 NAR 30, NAR 30

Strain history: <- D.P. Clark, Carbondale, Illinois

NCBI tax ID(s): 562 (species)

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General

@ref: 2788

BacDive-ID: 4626

DSM-Number: 6722

keywords: Bacteria, mesophilic

description: Escherichia coli K12 NAR 30 is a mesophilic bacterium of the family Enterobacteriaceae.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history

  • @ref: 2788
  • history: <- D.P. Clark, Carbondale, Illinois

doi: 10.13145/bacdive4626.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 2788

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: K12 NAR 30, NAR 30

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2788MINERAL MEDIUM M9 for E. coli JM strains (DSMZ Medium 382)yeshttps://mediadive.dsmz.de/medium/382Name: MINERAL MEDIUM M9 for E. coli JM strains (DSMZ Medium 382) Composition: Na2HPO4 6.0 g/l KH2PO4 3.0 g/l Glucose 2.0 g/l NH4Cl 1.0 g/l NaCl 0.5 g/l Thiamine-HCl x 2 H2O 0.373301 g/l MgSO4 0.12037 g/l Proline 0.02 g/l CaCl2 0.011098 g/l Distilled water
2788LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://mediadive.dsmz.de/medium/381Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 2788
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836830911sorbitol+fermentation
6836816899D-mannitol+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleyes
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole+
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
2788+-+-----+--++-++---+
2788+-+-----+---++++---+
2788+-+-----+--++-++---+-
2788+-------+--++-+----+
2788+-+-----+--++-++---+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
2788+++----+++++++++-+-+

Safety information

risk assessment

  • @ref: 2788
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 2788

culture collection no.: DSM 6722

straininfo link

  • @ref: 74121
  • straininfo: 48277

Reference

@idauthorscataloguedoi/urltitle
2788Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6722)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6722
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
74121Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48277.1StrainInfo: A central database for resolving microbial strain identifiers