Strain identifier

BacDive ID: 4616

Type strain: No

Species: Escherichia coli

Strain Designation: K12 LEK12, LEK12, Stanford

Strain history: <- P. Nevers <- J.W. Lengeler, Univ. Osnabrück <- R. Schmitt, Univ. Regensburg <- E. Lederberg, Stanford <- E.L. Tatum

NCBI tax ID(s): 562 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2561

BacDive-ID: 4616

DSM-Number: 6255

keywords: mesophilic, Bacteria

description: Escherichia coli K12 LEK12 is a mesophilic bacterium that was isolated from faeces of reconvalescent diphteria patient in fall 1922.

NCBI tax id

  • NCBI tax id: 562
  • Matching level: species

strain history: <- P. Nevers <- J.W. Lengeler, Univ. Osnabrück <- R. Schmitt, Univ. Regensburg <- E. Lederberg, Stanford <- E.L. Tatum

doi: 10.13145/bacdive4616.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Enterobacteriaceae
  • genus: Escherichia
  • species: Escherichia coli
  • full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus coli

@ref: 2561

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Enterobacteriaceae

genus: Escherichia

species: Escherichia coli

full scientific name: Escherichia coli (Migula 1895) Castellani and Chalmers 1919

strain designation: K12 LEK12, LEK12, Stanford

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2561
  • name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/381
  • composition: Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

  • @ref: 2561
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose+fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose+fermentation
6836830911sorbitol+fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin-hydrolysis
6836827897tryptophan+energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole+

enzymes

@refvalueactivityec
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
2561+-+-----+--++-++-+-+
2561+-+-----+--++-++-+-+
2561+-+-----+--++-++-+-+-

Isolation, sampling and environmental information

isolation

  • @ref: 2561
  • sample type: faeces of reconvalescent diphteria patient in fall 1922
  • geographic location: Stanford
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 2561
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

External links

@ref: 2561

culture collection no.: DSM 6255

straininfo link

@refpassport
20218http://www.straininfo.net/strains/159877
20218http://www.straininfo.net/strains/159879

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2561Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6255)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6255
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68368Automatically annotated from API 20E