Strain identifier

BacDive ID: 4396

Type strain: Yes

Species: Erwinia papayae

Strain Designation: MTQ2-1

Strain history: CIP <- 2004, CFBP <- 1995, L. de Lapeyre: strain MTQ2-1

NCBI tax ID(s): 206499 (species)

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General

@ref: 6478

BacDive-ID: 4396

DSM-Number: 16540

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, plant pathogen

description: Erwinia papayae MTQ2-1 is an obligate aerobe, mesophilic, Gram-negative plant pathogen that was isolated from Carica papaya cv. local.

NCBI tax id

  • NCBI tax id: 206499
  • Matching level: species

strain history

@refhistory
6478<- M. Le Saux, INRA <- L. De Lapeyre
121538CIP <- 2004, CFBP <- 1995, L. de Lapeyre: strain MTQ2-1

doi: 10.13145/bacdive4396.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Erwiniaceae
  • genus: Erwinia
  • species: Erwinia papayae
  • full scientific name: Erwinia papayae Gardan et al. 2004

@ref: 6478

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Erwiniaceae

genus: Erwinia

species: Erwinia papayae

full scientific name: Erwinia papayae Gardan et al. 2004

strain designation: MTQ2-1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
29937negativerod-shaped
121538negativerod-shapedno

pigmentation

  • @ref: 29937
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6478NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
33232MEDIUM 596 - for Erwinia papayeyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (7.000g);Yeast extract (7.000 g);Peptone (7.000 g)
121538CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6478positivegrowth28mesophilic
29937positivegrowth25-30mesophilic
29937positiveoptimum28mesophilic
33232positivegrowth30mesophilic
121538positivegrowth30-37mesophilic
121538nogrowth5psychrophilic
121538nogrowth10psychrophilic
121538nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121538
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 29937
  • spore formation: no

observation

  • @ref: 29937
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2993722599arabinose+carbon source
2993728757fructose+carbon source
2993728260galactose+carbon source
2993717754glycerol+carbon source
2993737684mannose+carbon source
2993733942ribose+carbon source
2993717992sucrose+carbon source
2993727082trehalose+carbon source
6836830849L-arabinose+fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol+fermentation
6836816899D-mannitol+fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
12153829864mannitol-fermentation
12153816947citrate-carbon source
12153817234glucose+fermentation
12153817716lactose-fermentation
12153817632nitrate-reduction
12153816301nitrite-reduction
12153815792malonate-assimilation
121538132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 121538
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12153835581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
12153815688acetoin+
12153817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
121538oxidase-
121538beta-galactosidase-3.2.1.23
121538alcohol dehydrogenase-1.1.1.1
121538gelatinase-
121538catalase+1.11.1.6
121538lysine decarboxylase-4.1.1.18
121538ornithine decarboxylase-4.1.1.17
121538phenylalanine ammonia-lyase-4.3.1.24
121538tryptophan deaminase-
121538urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121538-++-------++--------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
6478+---+----+++++--+--+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121538+++++--+---------+-++----------+-+---------++----+-----+---++-------------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
6478Carica papaya cv. localCarica papayaMorne Vert, MartiniqueFranceFRAEurope
121538Carica papayaMartiniqueFranceFRAEurope1995

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Tree

Safety information

risk assessment

@refpathogenicity plantbiosafety levelbiosafety level comment
6478yes1Risk group (German classification)
1215381Risk group (French classification)

Sequence information

16S sequences

  • @ref: 29937
  • description: Erwinia papayae 16S ribosomal RNA gene, partial sequence
  • accession: AY131237
  • length: 1436
  • database: nuccore
  • NCBI tax ID: 206499

GC content

  • @ref: 29937
  • GC-content: 52.5

External links

@ref: 6478

culture collection no.: DSM 16540, CFBP 5189, NCPPB 4294, CIP 108341

straininfo link

  • @ref: 73887
  • straininfo: 111270

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14742466Erwinia papayae sp. nov., a pathogen of papaya (Carica papaya).Gardan L, Christen R, Achouak W, Prior PInt J Syst Evol Microbiol10.1099/ijs.0.02718-02004Base Composition, Caribbean Region, Carica/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Erwinia/classification/isolation & purification/*pathogenicity, France, Molecular Sequence Data, Phenotype, Phylogeny, Plant Diseases/microbiology, RNA, Ribosomal, 16S/geneticsGenetics
30743675First Record of Bacterial Crown Rot of Papaya (Carica papaya) Caused by an Erwinia papayae-Like Bacterium in the Kingdom of Tonga.Fullerton RA, Taufa L, Vanneste JL, Yu J, Cornish DA, Park DPlant Dis10.1094/PDIS-06-10-04552011

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
6478Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16540)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16540
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29937Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2630828776041
33232Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5953
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
73887Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID111270.1StrainInfo: A central database for resolving microbial strain identifiers
121538Curators of the CIPCollection of Institut Pasteur (CIP 108341)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108341