Strain identifier
BacDive ID: 432
Type strain: 
Species: Alicyclobacillus macrosporangiidus
Strain Designation: 5A-239-2O-A
Strain history: IAM 15370 <-- K. Goto 5-A239-2O-A.
NCBI tax ID(s): 392015 (species)
version 9.3 (current version)
General
@ref: 7312
BacDive-ID: 432
DSM-Number: 17980
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, motile, rod-shaped
description: Alicyclobacillus macrosporangiidus 5A-239-2O-A is an aerobe, spore-forming, thermophilic bacterium that was isolated from soil, crop field.
NCBI tax id
- NCBI tax id: 392015
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7312 | <- Keiichi Goto, 5-A239-2O-A |
| 67770 | IAM 15370 <-- K. Goto 5-A239-2O-A. |
doi: 10.13145/bacdive432.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Alicyclobacillaceae
- genus: Alicyclobacillus
- species: Alicyclobacillus macrosporangiidus
- full scientific name: Alicyclobacillus macrosporangiidus Goto et al. 2007
@ref: 7312
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Alicyclobacillaceae
genus: Alicyclobacillus
species: Alicyclobacillus macrosporangiidus
full scientific name: Alicyclobacillus macrosporangiidus Goto et al. 2007
strain designation: 5A-239-2O-A
type strain: yes
Morphology
cell morphology
- @ref: 31943
- gram stain: positive
- cell length: 05-06 µm
- cell width: 0.7-0.8 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 31943
- production: no
Culture and growth conditions
culture medium
- @ref: 7312
- name: BACILLUS ACIDOCALDARIUS MEDIUM (DSMZ Medium 13)
- growth: yes
- link: https://mediadive.dsmz.de/medium/13
- composition: Name: BACILLUS ACIDOCALDARIUS MEDIUM (DSMZ Medium 13) Composition: Agar 20.0 g/l Glucose 1.0 g/l Yeast extract 1.0 g/l KH2PO4 0.6 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 7312 | positive | growth | 50 |
| 31943 | positive | growth | 35-60 |
| 31943 | positive | optimum | 50-55 |
| 67770 | positive | growth | 50 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31943 | positive | growth | 4.0-4.5 | acidophile |
| 31943 | positive | optimum | 4.25 |
Physiology and metabolism
oxygen tolerance
- @ref: 31943
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 31943 | yes | |
| 125438 | yes | 91.334 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 31943 | NaCl | positive | growth | 0-5 % |
| 31943 | NaCl | positive | optimum | 2.5 % |
observation
- @ref: 67770
- observation: quinones: MK-7
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31943 | 22599 | arabinose | + | carbon source |
| 31943 | 33984 | fucose | + | carbon source |
| 31943 | 17754 | glycerol | + | carbon source |
| 31943 | 17716 | lactose | + | carbon source |
| 31943 | 17306 | maltose | + | carbon source |
| 31943 | 26546 | rhamnose | + | carbon source |
| 31943 | 17814 | salicin | + | carbon source |
| 31943 | 27082 | trehalose | + | carbon source |
| 31943 | 18222 | xylose | + | carbon source |
enzymes
- @ref: 31943
- value: catalase
- activity: +
- ec: 1.11.1.6
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 7312 | soil, crop field | Fujieda city | Japan | JPN | Asia |
| 67770 | Soil of a crop field in Fujieda City | Shizuoka Pref. | Japan | JPN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Field |
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
Safety information
risk assessment
- @ref: 7312
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7312
- description: Alicyclobacillus macrosporangiidus gene for 16S ribosomal RNA, partial sequence
- accession: AB264025
- length: 1526
- database: nuccore
- NCBI tax ID: 392015
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Alicyclobacillus macrosporangiidus DSM 17980 | GCA_900116805 | scaffold | ncbi | 392015 |
| 66792 | Alicyclobacillus macrosporangiidus strain DSM 17980 | 392015.5 | wgs | patric | 392015 |
| 66792 | Alicyclobacillus macrosporangiidus DSM 17980 | 2675903231 | draft | img | 392015 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 7312 | 62.5 | |
| 67770 | 62.5 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 67.696 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 91.711 | yes |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 79.266 | yes |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 91.334 | yes |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | yes | 61.444 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | yes | 72.405 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | yes | 66 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 65.3 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 72.8 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | aerobe | 66.5 |
External links
@ref: 7312
culture collection no.: DSM 17980, IAM 15370, JCM 21814
straininfo link
- @ref: 70111
- straininfo: 309421
literature
- topic: Phylogeny
- Pubmed-ID: 17551043
- title: Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov.
- authors: Goto K, Mochida K, Kato Y, Asahara M, Fujita R, An SY, Kasai H, Yokota A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.64692-0
- year: 2007
- mesh: Aerobiosis, Bacterial Proteins/genetics, Bacterial Typing Techniques, Beverages/*microbiology, Carbohydrate Metabolism, Carboxylic Acids/metabolism, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Genes, rRNA, Gram-Positive Endospore-Forming Bacteria/*classification/cytology/*isolation & purification/physiology, Guaiacol/metabolism, Hydrogen-Ion Concentration, Microscopy, Phase-Contrast, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Temperature
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 7312 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17980) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17980 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 31943 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28199 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
| 70111 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID309421.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | ||||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |