Strain identifier
BacDive ID: 4298
Type strain: 
Species: Thiohalospira alkaliphila
Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ALgr 6sp
NCBI tax ID(s): 476279 (species)
version 8.1 (current version)
General
@ref: 6765
BacDive-ID: 4298
DSM-Number: 17116
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, motile, rod-shaped
description: Thiohalospira alkaliphila DSM 17116 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from sediments of hypersaline alkaline lakes.
NCBI tax id
- NCBI tax id: 476279
- Matching level: species
strain history
- @ref: 6765
- history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ALgr 6sp
doi: 10.13145/bacdive4298.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Chromatiales
- family: Ectothiorhodospiraceae
- genus: Thiohalospira
- species: Thiohalospira alkaliphila
- full scientific name: Thiohalospira alkaliphila Sorokin et al. 2008
@ref: 6765
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Chromatiales
family: Ectothiorhodospiraceae
genus: Thiohalospira
species: Thiohalospira alkaliphila
full scientific name: Thiohalospira alkaliphila Sorokin et al. 2008
type strain: yes
Morphology
cell morphology
- @ref: 32537
- gram stain: negative
- cell length: 02-04 µm
- cell width: 0.4-0.5 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 32537
- production: yes
Culture and growth conditions
culture medium
- @ref: 6765
- name: THIOHALOSPIRA ALKALIPHILA MEDIUM (DSMZ Medium 1229)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1229
- composition: Name: THIOHALOSPIRA ALKALIPHILA MEDIUM (DSMZ Medium 1229) Composition: NaCl 120.0 g/l Sodium thiosulfate pentahydrate 4.96372 g/l K2HPO4 2.0 g/l (NH4)2SO4 0.5 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l EDTA 5e-06 g/l FeSO4 x 7 H2O 2e-06 g/l MgCl2 x 6 H2O Vitamin B12 NaHCO3 Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6765 | positive | growth | 28 | mesophilic |
| 32537 | positive | growth | 35 | mesophilic |
| 32537 | positive | optimum | 35 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 32537 | positive | growth | 7-10.2 | alkaliphile |
| 32537 | positive | optimum | 8.5 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 6765 | microaerophile |
| 32537 | aerobe |
spore formation
- @ref: 32537
- spore formation: no
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 32537 | NaCl | positive | growth | 2.9-23.2 % |
| 32537 | NaCl | positive | optimum | 11.6 % |
observation
- @ref: 32537
- observation: aggregates in chains
Isolation, sampling and environmental information
isolation
- @ref: 6765
- sample type: sediments of hypersaline alkaline lakes
- geographic location: Libyan Desert, Wadi El Natrun
- country: Egypt
- origin.country: EGY
- continent: Africa
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Alkaline | |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_165306.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_56;96_61913;97_80221;98_108565;99_165306&stattab=map
- Last taxonomy: Thiohalospira alkaliphila subclade
- 16S sequence: EU169227
- Sequence Identity:
- Total samples: 816
- soil counts: 59
- aquatic counts: 717
- animal counts: 29
- plant counts: 11
Safety information
risk assessment
- @ref: 6765
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6765
- description: Thiohalospira alkaliphila strain ALgr 6sp 16S ribosomal RNA gene, partial sequence
- accession: EU169227
- length: 1318
- database: ena
- NCBI tax ID: 476279
GC content
| @ref | GC-content | method |
|---|---|---|
| 6765 | 65.6 | thermal denaturation, midpoint method (Tm) |
| 32537 | 65.6 |
External links
@ref: 6765
culture collection no.: DSM 17116, UNIQEM U372, ALgr 6sp
straininfo link
- @ref: 73790
- straininfo: 399817
literature
- topic: Phylogeny
- Pubmed-ID: 18599717
- title: Thiohalospira halophila gen. nov., sp. nov. and Thiohalospira alkaliphila sp. nov., novel obligately chemolithoautotrophic, halophilic, sulfur-oxidizing gammaproteobacteria from hypersaline habitats.
- authors: Sorokin DY, Tourova TP, Muyzer G, Kuenen GJ
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65654-0
- year: 2008
- mesh: *Chemoautotrophic Growth, Ecosystem, Ectothiorhodospiraceae/*classification/genetics/*physiology/ultrastructure, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Salts/*metabolism, Species Specificity, Sulfur/*metabolism
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6765 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17116) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17116 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 32537 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28755 | 28776041 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 73790 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID399817.1 | StrainInfo: A central database for resolving microbial strain identifiers |