Archive BacDiveID:10.13145/bacdive4298.20240510.9

Strain identifier

BacDive ID: 4298

Type strain:

Species: Thiohalospira alkaliphila

Strain history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ALgr 6sp

NCBI tax ID(s): 476279 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 6765

BacDive-ID: 4298

DSM-Number: 17116

keywords: 16S sequence, Bacteria, microaerophile, Gram-negative, motile, rod-shaped

description: Thiohalospira alkaliphila DSM 17116 is a microaerophile, Gram-negative, motile bacterium that was isolated from sediments of hypersaline alkaline lakes.

NCBI tax id

NCBI tax id: 476279
Matching level: species

strain history

@ref: 6765
history: <- D. Y. Sorokin, Dept. Biotechnol., Delft Univ. Technol., Netherlands; ALgr 6sp

doi: 10.13145/bacdive4298.20240510.9

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Chromatiales
family: Ectothiorhodospiraceae
genus: Thiohalospira
species: Thiohalospira alkaliphila
full scientific name: Thiohalospira alkaliphila Sorokin et al. 2008

@ref: 6765

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Ectothiorhodospiraceae

genus: Thiohalospira

species: Thiohalospira alkaliphila

full scientific name: Thiohalospira alkaliphila Sorokin et al. 2008

type strain: yes

Morphology

cell morphology

@ref: 32537
gram stain: negative
cell length: 02-04 µm
cell width: 0.4-0.5 µm
cell shape: rod-shaped
motility: yes

pigmentation

@ref: 32537
production: yes

Culture and growth conditions

culture medium

@ref: 6765
name: THIOHALOSPIRA ALKALIPHILA MEDIUM (DSMZ Medium 1229)
growth: yes
link: https://mediadive.dsmz.de/medium/1229
composition: Name: THIOHALOSPIRA ALKALIPHILA MEDIUM (DSMZ Medium 1229) Composition: NaCl 120.0 g/l Sodium thiosulfate pentahydrate 4.96372 g/l K2HPO4 2.0 g/l (NH4)2SO4 0.5 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l EDTA 5e-06 g/l FeSO4 x 7 H2O 2e-06 g/l MgCl2 x 6 H2O Vitamin B12 NaHCO3 Distilled water

culture temp

@ref	growth	type	temperature
6765	positive	growth	28
32537	positive	growth	35
32537	positive	optimum	35

culture pH

@ref	ability	type	pH	PH range
32537	positive	growth	7-10.2	alkaliphile
32537	positive	optimum	8.5

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance
6765	microaerophile
32537	aerobe

spore formation

@ref: 32537
spore formation: no

halophily

@ref	salt	growth	tested relation	concentration
32537	NaCl	positive	growth	2.9-23.2 %
32537	NaCl	positive	optimum	11.6 %

observation

@ref: 32537
observation: aggregates in chains

Isolation, sampling and environmental information

isolation

@ref: 6765
sample type: sediments of hypersaline alkaline lakes
geographic location: Libyan Desert, Wadi El Natrun
country: Egypt
origin.country: EGY
continent: Africa

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline
#Condition	#Saline

taxonmaps

@ref: 69479
File name: preview.99_165306.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_56;96_61913;97_80221;98_108565;99_165306&stattab=map
Last taxonomy: Thiohalospira alkaliphila subclade
16S sequence: EU169227
Sequence Identity:
Total samples: 816
soil counts: 59
aquatic counts: 717
animal counts: 29
plant counts: 11

Safety information

risk assessment

@ref: 6765
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 6765
description: Thiohalospira alkaliphila strain ALgr 6sp 16S ribosomal RNA gene, partial sequence
accession: EU169227
length: 1318
database: nuccore
NCBI tax ID: 476279

GC content

@ref	GC-content	method
6765	65.6	thermal denaturation, midpoint method (Tm)
32537	65.6

External links

@ref: 6765

culture collection no.: DSM 17116, UNIQEM U372, ALgr 6sp

straininfo link

@ref: 73790
straininfo: 399817

literature

topic: Phylogeny
Pubmed-ID: 18599717
title: Thiohalospira halophila gen. nov., sp. nov. and Thiohalospira alkaliphila sp. nov., novel obligately chemolithoautotrophic, halophilic, sulfur-oxidizing gammaproteobacteria from hypersaline habitats.
authors: Sorokin DY, Tourova TP, Muyzer G, Kuenen GJ
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.65654-0
year: 2008
mesh: *Chemoautotrophic Growth, Ecosystem, Ectothiorhodospiraceae/*classification/genetics/*physiology/ultrastructure, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Salts/*metabolism, Species Specificity, Sulfur/*metabolism
topic2: Genetics

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed
6765	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17116)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-17116
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32537	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	28755	28776041
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
73790	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID399817.1	StrainInfo: A central database for resolving microbial strain identifiers