Strain identifier

BacDive ID: 4064

Type strain: Yes

Species: Desulfonatronum thiodismutans

Strain history: CIP <- 2003, E. Pikuta, NSSTC, Huntsville, AL, USA: strain MLF1

NCBI tax ID(s): 1121414 (strain), 159290 (species)

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General

@ref: 5494

BacDive-ID: 4064

DSM-Number: 14708

keywords: 16S sequence, Bacteria, anaerobe, lithoheterotroph, mesophilic, Gram-negative, motile

description: Desulfonatronum thiodismutans DSM 14708 is an anaerobe, lithoheterotroph, mesophilic bacterium that was isolated from mud sediments of alkaline, hypersaline, soda lake.

NCBI tax id

NCBI tax idMatching level
159290species
1121414strain

strain history

@refhistory
5494<- E. V. Pikuta, NASA/NSSTC, Huntsville, USA; MLF1 <- E. V. Pikuta {2000}
407272003, E. Pikuta, NSSTC, Huntsville, AL, USA: strain MLF1
123521CIP <- 2003, E. Pikuta, NSSTC, Huntsville, AL, USA: strain MLF1

doi: 10.13145/bacdive4064.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Desulfovibrionales
  • family: Desulfonatronaceae
  • genus: Desulfonatronum
  • species: Desulfonatronum thiodismutans
  • full scientific name: Desulfonatronum thiodismutans Pikuta et al. 2003

@ref: 5494

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Desulfovibrionales

family: Desulfonatronaceae

genus: Desulfonatronum

species: Desulfonatronum thiodismutans

full scientific name: Desulfonatronum thiodismutans Pikuta et al. 2003

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthmotilityflagellum arrangementcell shape
23209negative1.2-2.7 µm0.6-0.7 µmyespolar
23209vibrio-shaped
123521negativenorod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5494DESULFONATRONUM THIODISMUTANS MEDIUM (DSMZ Medium 813a)yeshttps://mediadive.dsmz.de/medium/813aName: DESULFONATRONUM THIODISMUTANS MEDIUM (DSMZ Medium 813a) Composition: NaCl 29.9401 g/l Na2SO4 4.99002 g/l Na-formate 3.99202 g/l Na2CO3 3.49301 g/l NH4Cl 0.998004 g/l Yeast extract 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KCl 0.199601 g/l KH2PO4 0.199601 g/l MgCl2 x 6 H2O 0.0998004 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water
40727MEDIUM 224 - for anaerobic bacteria TGVyesDistilled water make up to (1000.000 ml);Glucose (10.000 g);Yeast extract (20.000 g);Resazurin (2.000 mg);Trypto casein soy broth (30.000 g);L-Cysteine (0.500 g);Hemin solution - M00149 (25.000 ml);Vitamins solution - M00850 (10.000 ml)
123521CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20
123521CIP Medium 476yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=476

culture temp

@refgrowthtypetemperaturerange
5494positivegrowth35mesophilic
23209positivegrowth15-48
23209positiveoptimum37mesophilic
40727positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
23209positivegrowth8.0-10.0alkaliphile
23209positiveoptimum9.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5494anaerobe
23209anaerobe

nutrition type

  • @ref: 23209
  • type: lithoheterotroph

spore formation

  • @ref: 23209
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23209NaClpositivegrowth1-7 %(w/v)
23209NaClpositiveoptimum3 %(w/v)
23209haloalkaliphilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2320933403elemental sulfur-electron acceptor
2320917632nitrate-electron acceptor
2320933403elemental sulfur-growth
2320936264molybdate-growth
2320916189sulfate-growth
2320916189sulfate+electron acceptor
2320917359sulfite+electron acceptor
2320916094thiosulfate+electron acceptor
2320916236ethanol+electron donor
2320915740formate+electron donor
23209yeast extract+growth

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2320917833gentamicinyesyes
232096104kanamycinyesyes
2320917698chloramphenicolyesyes
2320927902tetracyclineyesyes

enzymes

  • @ref: 23209
  • value: catalase
  • activity: -
  • ec: 1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5494mud sediments of alkaline, hypersaline, soda lakeCalifornia, Mono LakeUSAUSANorth America
23209sediments of alkaline, hypersaline, soda Mono Lake
123521Environment, Sediments of soda Mono LakeCaliforniaUnited States of AmericaUSANorth America2000

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment
#Condition#Alkaline
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_4226.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_453;96_2133;97_2569;98_3184;99_4226&stattab=map
  • Last taxonomy: Desulfonatronum thiodismutans
  • 16S sequence: AF373920
  • Sequence Identity:
  • Total samples: 28
  • soil counts: 2
  • aquatic counts: 25
  • animal counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
54941Risk group (German classification)
1235211Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5494
  • description: Desulfonatronum thiodismutans strain MLF1 16S ribosomal RNA gene, partial sequence
  • accession: AF373920
  • length: 1481
  • database: ena
  • NCBI tax ID: 159290

GC content

@refGC-contentmethod
549463high performance liquid chromatography (HPLC)
2320963.0high performance liquid chromatography (HPLC)

External links

@ref: 5494

culture collection no.: DSM 14708, ATCC BAA 395, MLF 1, CIP 107913

straininfo link

  • @ref: 73567
  • straininfo: 127989

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130014Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth.Pikuta EV, Hoover RB, Bej AK, Marsic D, Whitman WB, Cleland D, Krader PInt J Syst Evol Microbiol10.1099/ijs.0.02598-02003Base Composition, California, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Deltaproteobacteria/*classification/genetics/growth & development/metabolism, Fatty Acids/analysis, Geologic Sediments/microbiology, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sulfates/metabolismMetabolism
Genetics25081260Draft Genome Sequence of the Obligately Alkaliphilic Sulfate-Reducing Bacterium Desulfonatronum thiodismutans Strain MLF1.Trubitsyn D, Geurink C, Pikuta E, Lefevre CT, McShan WM, Gillaspy AF, Bazylinski DAGenome Announc10.1128/genomeA.00741-142014

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5494Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14708)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14708
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23209Elena V. Pikuta, Richard B. Hoover, Asim K. Bej, Damien Marsic, William B. Whitman, David Cleland, Paul Krader10.1099/ijs.0.02598-0Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growthIJSEM 53: 1327-1332 200313130014
40727Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5477
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
73567Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127989.1StrainInfo: A central database for resolving microbial strain identifiers
123521Curators of the CIPCollection of Institut Pasteur (CIP 107913)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107913