Strain identifier
BacDive ID: 398
Type strain: 
Species: Kangiella spongicola
Strain Designation: A79
Strain history: <- M. Häggblom, Rutgers, State Univ. New Jersey, New Brunswick, USA; A79 <- J. Ahn
NCBI tax ID(s): 796379 (species)
version 8.1 (current version)
General
@ref: 16755
BacDive-ID: 398
DSM-Number: 23219
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Kangiella spongicola A79 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sponge Chondrilla nucula.
NCBI tax id
- NCBI tax id: 796379
- Matching level: species
strain history
- @ref: 16755
- history: <- M. Häggblom, Rutgers, State Univ. New Jersey, New Brunswick, USA; A79 <- J. Ahn
doi: 10.13145/bacdive398.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Oceanospirillales
- family: Kangiellaceae
- genus: Kangiella
- species: Kangiella spongicola
- full scientific name: Kangiella spongicola Ahn et al. 2011
@ref: 16755
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Oceanospirillales
family: Kangiellaceae
genus: Kangiella
species: Kangiella spongicola
full scientific name: Kangiella spongicola Ahn et al. 2011
strain designation: A79
type strain: yes
Morphology
cell morphology
- @ref: 29816
- gram stain: negative
- cell length: 2.25 µm
- cell width: 0.75 µm
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
- @ref: 16755
- name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514
- composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16755 | positive | growth | 28 | mesophilic |
| 29816 | positive | growth | 10-49 | |
| 29816 | positive | optimum | 29.5 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29816 | positive | growth | 4.5-10 | alkaliphile |
| 29816 | positive | optimum | 7.25 |
Physiology and metabolism
oxygen tolerance
- @ref: 29816
- oxygen tolerance: aerobe
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 29816 | NaCl | positive | growth | 2.0-15 % |
| 29816 | NaCl | positive | optimum | 6 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29816 | 53424 | tween 20 | + | carbon source |
| 29816 | 53426 | tween 80 | + | carbon source |
| 29816 | 17632 | nitrate | + | reduction |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29816 | acid phosphatase | + | 3.1.3.2 |
| 29816 | alkaline phosphatase | + | 3.1.3.1 |
| 29816 | catalase | + | 1.11.1.6 |
| 29816 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
- @ref: 16755
- sample type: sponge Chondrilla nucula
- host species: Chondrilla nucula
- geographic location: Florida Keys
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
- Cat1: #Host
- Cat2: #Invertebrates (Other)
- Cat3: #Porifera (Sponges)
Safety information
risk assessment
- @ref: 16755
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16755
- description: Kangiella spongicola strain A79 16S ribosomal RNA gene, partial sequence
- accession: GU339304
- length: 1473
- database: ena
- NCBI tax ID: 796379
GC content
| @ref | GC-content | method |
|---|---|---|
| 16755 | 44.9 | high performance liquid chromatography (HPLC) |
| 29816 | 44.9 |
External links
@ref: 16755
culture collection no.: DSM 23219, ATCC BAA 2076
straininfo link
- @ref: 70078
- straininfo: 399230
literature
- topic: Phylogeny
- Pubmed-ID: 20511465
- title: Kangiella spongicola sp. nov., a halophilic marine bacterium isolated from the sponge Chondrilla nucula.
- authors: Ahn J, Park JW, McConnell JA, Ahn YB, Haggblom MM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.021733-0
- year: 2010
- mesh: Aerobiosis, Animals, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Florida, Gammaproteobacteria/*classification/genetics/*isolation & purification/physiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Phylogeny, Porifera/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 16755 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23219) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-23219 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29816 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26193 | 28776041 | |
| 70078 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID399230.1 | StrainInfo: A central database for resolving microbial strain identifiers |