Strain identifier
BacDive ID: 3948
Type strain: 
Species: Desulfatiferula olefinivorans
Strain Designation: LM2801
Strain history: A. Hirschler-Réa LM2801.
NCBI tax ID(s): 396322 (species)
version 8.1 (current version)
General
@ref: 7736
BacDive-ID: 3948
DSM-Number: 18843
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Desulfatiferula olefinivorans LM2801 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from oil-polluted sediment.
NCBI tax id
- NCBI tax id: 396322
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7736 | <- A. Hirschler-Réa, IBEAS Université de Pau et des Pays de l'Adour, BP1155, 64013 Pau cedex, France; LM2801 <- C. Cravo-Laureau {2001} |
| 67770 | A. Hirschler-Réa LM2801. |
doi: 10.13145/bacdive3948.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Desulfobacterales
- family: Desulfobacteraceae
- genus: Desulfatiferula
- species: Desulfatiferula olefinivorans
- full scientific name: Desulfatiferula olefinivorans Cravo-Laureau et al. 2007
@ref: 7736
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Desulfobacterales
family: Desulfobacteraceae
genus: Desulfatiferula
species: Desulfatiferula olefinivorans
full scientific name: Desulfatiferula olefinivorans Cravo-Laureau et al. 2007
strain designation: LM2801
type strain: yes
Morphology
cell morphology
- @ref: 32274
- gram stain: negative
- cell length: 2.9 µm
- cell width: 0.45 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 7736
- incubation period: >14 days
Culture and growth conditions
culture medium
- @ref: 7736
- name: DESULFOBACTER MEDIUM (DSMZ Medium 193)
- growth: yes
- link: https://mediadive.dsmz.de/medium/193
- composition: Name: DESULFOBACTER MEDIUM (DSMZ Medium 193; with strain-specific modifications) Composition: NaCl 6.97906 g/l Na2SO4 2.99102 g/l Na2CO3 1.49551 g/l MgCl2 x 6 H2O 1.29611 g/l Na-stearate 0.618146 g/l KCl 0.498505 g/l Na2S x 9 H2O 0.398804 g/l NH4Cl 0.299102 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149552 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Pyridoxine hydrochloride 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7736 | positive | growth | 30 | mesophilic |
| 32274 | positive | growth | 16-38 | |
| 32274 | positive | optimum | 33 | mesophilic |
| 67770 | positive | growth | 35 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 32274 | positive | growth | 6.6-8.3 |
| 32274 | positive | optimum | 7.5 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 7736 | anaerobe |
| 32274 | anaerobe |
spore formation
- @ref: 32274
- spore formation: no
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 7736 | oil-polluted sediment | Berre lagoon | France | FRA | Europe |
| 67770 | Brackish sediment of a wastewater decantation facility of an oil refinery in Berre lagoon | France | FRA | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | #Oil (Fuel) |
| #Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
- @ref: 7736
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7736
- description: Desulfatiferula olefinivorans strain LM2801 16S ribosomal RNA gene, partial sequence
- accession: DQ826724
- length: 1554
- database: ena
- NCBI tax ID: 396322
GC content
| @ref | GC-content | method |
|---|---|---|
| 7736 | 45.5 | |
| 67770 | 45.5 | high performance liquid chromatography (HPLC) |
External links
@ref: 7736
culture collection no.: DSM 18843, JCM 14469
straininfo link
- @ref: 73457
- straininfo: 319370
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 17978243 | Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium. | Cravo-Laureau C, Labat C, Joulian C, Matheron R, Hirschler-Rea A | Int J Syst Evol Microbiol | 10.1099/ijs.0.65240-0 | 2007 | Alkenes/chemistry/*metabolism, Bacterial Typing Techniques, Biodegradation, Environmental, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Deltaproteobacteria/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, *Petroleum, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification/physiology, Waste Disposal, Fluid/*methods, *Water Microbiology | Enzymology |
| Metabolism | 21810468 | Anaerobic oxidation of n-alkenes by sulphate-reducing bacteria from the genus Desulfatiferula: n-ketones as potential metabolites. | Grossi V, Cravo-Laureau C, Rontani JF, Cros M, Hirschler-Rea A | Res Microbiol | 10.1016/j.resmic.2011.07.004 | 2011 | Alkenes/*metabolism, Anaerobiosis, Biodegradation, Environmental, Fatty Acids, Unsaturated/*metabolism, Gas Chromatography-Mass Spectrometry, Ketones/*metabolism, Lipid Metabolism, Oxidation-Reduction, Sulfates/metabolism, Sulfur-Reducing Bacteria/*metabolism |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 7736 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18843) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18843 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 32274 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28514 | 28776041 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 73457 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID319370.1 | StrainInfo: A central database for resolving microbial strain identifiers |