Strain identifier

BacDive ID: 366

Type strain: Yes

Species: Taylorella equigenitalis

Strain Designation: 61717/77

Strain history: CIP <- 1979, NCTC, Haemophilus equigenitalis <- 1977, C.E.D. Taylor, Pub. Hlth. Lab, Cambridge, UK: strain 61717/77

NCBI tax ID(s): 743973 (strain), 29575 (species)

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General

@ref: 4030

BacDive-ID: 366

DSM-Number: 10668

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, animal pathogen

description: Taylorella equigenitalis 61717/77 is a microaerophile, mesophilic animal pathogen that was isolated from contagious equine metritis.

NCBI tax id

NCBI tax idMatching level
743973strain
29575species

strain history

@refhistory
4030<- NCTC <- C. E. D. Taylor, PHLS
119120CIP <- 1979, NCTC, Haemophilus equigenitalis <- 1977, C.E.D. Taylor, Pub. Hlth. Lab, Cambridge, UK: strain 61717/77

doi: 10.13145/bacdive366.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Taylorella
  • species: Taylorella equigenitalis
  • full scientific name: Taylorella equigenitalis (Taylor et al. 1983) Sugimoto et al. 1984

  • synonyms

    • @ref: 20215
    • synonym: Haemophilus equigenitalis

@ref: 4030

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Taylorella

species: Taylorella equigenitalis

full scientific name: Taylorella equigenitalis (Taylor et al. 1983) Sugimoto et al. 1984

strain designation: 61717/77

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.901
6948099.944negative
119120nonegativerod-shaped

colony morphology

  • @ref: 4030
  • type of hemolysis: gamma
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4030COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429; with strain-specific modifications) Composition: Horse blood 100.0 g/l Columbia agar base
37712MEDIUM 82 - for Taylorella equigenitalis and Bartonella clarridgeiaeyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Rabbitblood (50.000 ml)
119120CIP Medium 82yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=82

culture temp

@refgrowthtypetemperaturerange
4030positivegrowth37mesophilic
37712positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4030microaerophile
119120facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11912017632nitrate-reduction
11912016301nitrite-reduction
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11912035581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4030catalase+1.11.1.6
4030cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68377tryptophan deaminase-4.1.99.1
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
119120oxidase+
119120catalase+1.11.1.6
119120urease-3.5.1.5

fatty acid profile

  • @reffatty acidpercentageECL
    45339C12:00.612
    45339C14:0214
    45339C16:03416
    45339C18:0518
    45339C12:0 ALDE ?210.915
    45339C14:0 3OH/C16:1 ISO I6.715.485
    45339C15:1 ω8c1.214.792
    45339C16:1 ω7c2.415.819
    45339C18:1 ω7c /12t/9t43.817.824
    45339C18:1 ω9c0.917.769
    45339C18:2 ω6,9c/C18:0 ANTE117.724
    45339C19:0 10-methyl0.519.368
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    45339C14:01.514
    45339C16:026.816
    45339C18:03.318
    45339C12:0 ALDE ?1.510.915
    45339C14:0 3OH/C16:1 ISO I6.615.485
    45339C15:1 ω8c1.114.792
    45339C16:1 ω7c1.815.819
    45339C18:1 ω7c /12t/9t53.717.824
    45339C18:1 ω9c1.317.769
    45339C18:2 ω6,9c/C18:0 ANTE1.517.724
    45339C19:0 10-methyl119.368
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119120-+++-+-----+--+-----

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
4030-------+--+--
4030-------++/--+--
4030-------+-----
4030-+/-------+--+-
4030--------+--+-
4030--------++/--+-
4030--------+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
4030contagious equine metritisCambridgeUnited KingdomGBREurope52.20530.1192
45339Equine,CEM:Contagious equine metritisUnited KingdomGBREurope1977
119120Animal, Equine, contagious metritisUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Urogenital tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_2624.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_1399;97_1670;98_2032;99_2624&stattab=map
  • Last taxonomy: Taylorella equigenitalis subclade
  • 16S sequence: X68645
  • Sequence Identity:
  • Total samples: 1076
  • soil counts: 190
  • aquatic counts: 374
  • animal counts: 453
  • plant counts: 59

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
4030yes2Risk group (German classification)
1191201Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Taylorella equigenitalis strain ATCC 35865 16S ribosomal RNA gene, partial sequenceDQ0995531451ena743973
20218Taylorella equigenitalis genes for 16S ribosomal RNA, tRNA-Ile, tRNA-Ala, 23S ribosomal RNA, partial and complete sequence, strain: NCTC 11184AB1136531026ena743973
20218Taylorella equigenitalis genes for 16S ribosomal RNA, tRNA-Ile, tRNA-Ala, 23S ribosomal RNA, partial and complete sequence, strain: NCTC 11184AB113656932ena743973
20218Taylorella equigenitalis strain NCTC11184 16S ribosomal RNA gene, complete sequenceAF4081971499ena743973
4030T.equigenitalis gene for 16S rRNAX686451499ena743973

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Taylorella equigenitalis ATCC 35865 NCTC 11184GCA_900637125completencbi743973
66792Taylorella equigenitalis ATCC 35865GCA_000276685completencbi743973
66792Taylorella equigenitalis ATCC 35865743973.6wgspatric743973
66792Taylorella equigenitalis ATCC 35865743973.3completepatric743973
66792Taylorella equigenitalis ATCC 35865 strain NCTC11184 strain NCTC 11184743973.8completepatric743973
66792Taylorella equigenitalis ATCC 358652517093031completeimg743973

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno95.682no
gram-positiveno98.806no
anaerobicno94.769no
aerobicno84.842no
halophileno81.887no
spore-formingno97.762no
thermophileno96.584yes
glucose-utilno78.533no
motileno84.458no
glucose-fermentno90.308no

External links

@ref: 4030

culture collection no.: DSM 10668, ATCC 35865, CIP 7909, NCTC 11184, CCUG 10786, LMG 6222, CIP 79.7

straininfo link

  • @ref: 70046
  • straininfo: 389411

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Cultivation99302The causative organism of contagious equine metritis 1977: proposal for a new species to be known as Haemophilus equigenitalis.Taylor CE, Rosenthal RO, Brown DF, Lapage SP, Hill LR, Legros RMEquine Vet J10.1111/j.2042-3306.1978.tb02242.x1978Agglutination Tests, Animals, Bacterial Infections/microbiology/*veterinary, Brucella abortus/immunology, Culture Media, DNA, Bacterial/analysis, Endometritis/microbiology/*veterinary, Female, Haemophilus/*classification/growth & development/immunology, Horse Diseases/*microbiology, Horses, Male, Pasteurella/immunology, *Terminology as TopicPhylogeny
Genetics7975202Analysis of chromosome-sized DNA and genome typing of isolated strains of Taylorella equigenitalis.Matsuda M, Asami Y, Miyazawa T, Samata T, Isayama Y, Honda M, Ide YVet Res Commun10.1007/BF018392251994Animals, Bacterial Typing Techniques, DNA, Bacterial/*analysis, *Genome, Bacterial, Haemophilus/*genetics, Horse Diseases/microbiology, HorsesPhylogeny
Enzymology9265772Profiles of fragments after pulsed-field gel electrophoresis of cleaved genomic DNA from strains of Taylorella equigenitalis isolated from horses in Norway.Matsuda M, Miyazawa T, Ishida Y, Moore JEMicrobiol Res10.1016/S0944-5013(97)80015-81997Animals, DNA, Bacterial/*genetics, Electrophoresis, Gel, Pulsed-Field, Endometritis/microbiology/*veterinary, Female, Genotype, Haemophilus/classification/*genetics, Horse Diseases/*microbiology, Horses, NorwayGenetics
Enzymology9686436Molecular genotyping by pulsed-field gel electrophoresis of restricted genomic DNA of strains of Taylorella equigenitalis isolated in Ireland and in the United States.Matsuda M, Miyazawa T, Moore JE, Buckley TC, Thomas LAVet Res Commun10.1023/a:10060022126041998Animals, DNA, Bacterial/*analysis/chemistry, Deoxyribonucleases, Type II Site-Specific/chemistry, Electrophoresis, Gel, Pulsed-Field/veterinary, Endometritis/microbiology/*veterinary, Female, Genotype, Haemophilus/*classification/genetics, Haemophilus Infections/microbiology/*veterinary, Horse Diseases/*microbiology, Horses, Ireland, Sexually Transmitted Diseases, Bacterial/microbiology/*veterinary, United StatesGenetics
Genetics10335605Detection of a common genotype among strains of Taylorella equigenitalis isolated from thoroughbred horses in Japan between 1994 and 1996.Matsuda M, Miyazawa T, Anzai TJ Basic Microbiol10.1002/(sici)1521-4028(199905)39:2<127::aid-jobm127>3.0.co;2-q1999Animals, Genotype, Haemophilus/*classification/isolation & purification, Horses/*microbiology, Japan, Time FactorsPhylogeny
Phylogeny16502103Nucleotide sequencing and analysis of 16S rDNA and 16S-23S rDNA internal spacer region (ISR) of Taylorella equigenitalis, as an important pathogen for contagious equine metritis (CEM).Kagawa S, Nagano Y, Tazumi A, Murayama O, Millar BC, Moore JE, Matsuda MVet Res Commun10.1007/s11259-006-3304-62006Animals, Base Sequence, DNA, Ribosomal Spacer/*genetics, Gram-Negative Bacterial Infections/microbiology/*veterinary, Horse Diseases/*microbiology, Horses, Molecular Sequence Data, RNA, Ribosomal, 16S/*genetics, RNA, Ribosomal, 23S/*genetics, Taylorella equigenitalis/*geneticsGenetics
Enzymology18950750The structure of the polysaccharide of the lipopolysaccharide produced by Taylorella equigenitalis type strain (ATCC 35865).Vinogradov E, MacLean LL, Brooks BW, Lutze-Wallace C, Perry MBCarbohydr Res10.1016/j.carres.2008.09.0152008Bacterial Vaccines/chemistry/immunology, Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Humans, Immune Sera/immunology, Lipopolysaccharides/*biosynthesis/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, O Antigens/*chemistry/immunology, Species Specificity, Spectrometry, Mass, Electrospray Ionization, Taylorella equigenitalis/immunology/isolation & purification/*metabolismPhylogeny
Genetics19381472Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis.Tazumi A, Sekizuka T, Moore JE, Millar BC, Taneike I, Matsuda MFolia Microbiol (Praha)10.1007/s12223-008-0076-02009Animals, Base Sequence, Consensus Sequence, *Genes, Bacterial, Gram-Negative Bacterial Infections/microbiology/veterinary, Horse Diseases/microbiology, Horses, Introns/*genetics, Molecular Sequence Data, Nucleic Acid Conformation, *RNA Processing, Post-Transcriptional, RNA, Bacterial/*genetics, RNA, Ribosomal, 23S/*genetics, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity, Taylorella equigenitalis/*geneticsPhylogeny
Genetics22541164Comparative genomic analyses of the Taylorellae.Hauser H, Richter DC, van Tonder A, Clark L, Preston AVet Microbiol10.1016/j.vetmic.2012.03.0412012Adhesins, Bacterial/genetics, Bacterial Proteins/*genetics, Fimbriae Proteins/genetics, Genetic Variation, Genome, Bacterial/*genetics, Sequence Homology, Nucleic Acid, Taylorella/classification/*genetics/metabolismPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4030Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10668)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10668
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37712Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11030
45339Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 10786)https://www.ccug.se/strain?id=10786
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70046Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389411.1StrainInfo: A central database for resolving microbial strain identifiers
119120Curators of the CIPCollection of Institut Pasteur (CIP 79.7)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2079.7