Strain identifier

BacDive ID: 349

Type strain: Yes

Species: Castellaniella caeni

Strain Designation: Ho-11

Strain history: <- ST Lee, KAIST

NCBI tax ID(s): 266123 (species)

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General

@ref: 16223

BacDive-ID: 349

DSM-Number: 22240

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Castellaniella caeni Ho-11 is a facultative anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from sludge of the aerobic treatment tanks of a municipal leachate treatment plant.

NCBI tax id

  • NCBI tax id: 266123
  • Matching level: species

strain history

@refhistory
16223<- S. T. Lee, KAIST; Ho-11
67771<- ST Lee, KAIST

doi: 10.13145/bacdive349.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Castellaniella
  • species: Castellaniella caeni
  • full scientific name: Castellaniella caeni Liu et al. 2008

@ref: 16223

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Castellaniella

species: Castellaniella caeni

full scientific name: Castellaniella caeni Liu et al. 2008

strain designation: Ho-11

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23268negative1.2-2.0 µm0.3-0.5 µmrod-shapedno
67771negative

colony morphology

  • @ref: 23268
  • colony size: 1-2 mm
  • colony color: brownish, transparent
  • colony shape: circular

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16223R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23268MacConkey agaryes
23268Nutrient agar (NA)yes
23268Trypticase Soy Agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
16223positivegrowth28mesophilic
23268positivegrowth10-37
23268positiveoptimum30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23268positivegrowth5.0-8.5alkaliphile
23268positiveoptimum6.0-7.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23268facultative anaerobe
67771aerobe

halophily

  • @ref: 23268
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

observation

  • @ref: 67771
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2326827613amygdalin-builds acid from
2326816899D-mannitol-builds acid from
2326817924D-sorbitol-builds acid from
2326817234glucose-builds acid from
2326817268myo-inositol-builds acid from
2326830849L-arabinose-builds acid from
2326862345L-rhamnose-builds acid from
2326828053melibiose-builds acid from
2326817992sucrose-builds acid from
23268168082-dehydro-D-gluconate-growth
23268161933-hydroxybenzoate-growth
23268178794-hydroxybenzoate-growth
23268174265-dehydro-D-gluconate-growth
2326827613amygdalin-growth
2326816150benzoate-growth
2326816947citrate-growth
2326817716lactose-growth
2326862318D-lyxose-growth
2326816899D-mannitol-growth
2326816024D-mannose-growth
2326816988D-ribose-growth
2326817924D-sorbitol-growth
2326865327D-xylose-growth
2326852071dextran-growth
2326816813galactitol-growth
2326816236ethanol-growth
2326828757fructose-growth
2326824265gluconate-growth
2326828087glycogen-growth
2326817268myo-inositol-growth
2326815443inulin-growth
2326817240itaconate-growth
2326830849L-arabinose-growth
2326815603L-leucine-growth
2326816643L-methionine-growth
2326817295L-phenylalanine-growth
2326862345L-rhamnose-growth
2326817266L-sorbose-growth
2326816828L-tryptophan-growth
2326817895L-tyrosine-growth
2326865328L-xylose-growth
2326818300maleic acid-growth
2326815792malonate-growth
2326817306maltose-growth
2326828053melibiose-growth
23268506227N-acetylglucosamine-growth
2326830623oxalate-growth
2326815361pyruvate-growth
2326816634raffinose-growth
2326817814salicin-growth
2326817992sucrose-growth
23268132950tartrate-growth
2326827082trehalose-growth
2326817151xylitol-growth
2326885146carboxymethylcellulose-hydrolysis
23268casein-hydrolysis
2326817029chitin-hydrolysis
2326816991dna-hydrolysis
232685291gelatin-hydrolysis
2326828017starch-hydrolysis
2326837166xylan-hydrolysis
2326829991L-aspartate+growth
23268370543-hydroxybutyrate+growth
2326830089acetate+growth
2326817128adipate+growth
2326827689decanoate+growth
2326817057cellobiose+growth
2326815963ribitol+growth
2326817108D-arabinose+growth
2326812936D-galactose+growth
2326817634D-glucose+growth
2326815740formate+growth
2326829806fumarate+growth
2326817859glutaric acid+growth
2326817754glycerol+growth
2326815428glycine+growth
2326816977L-alanine+growth
2326816467L-arginine+growth
2326817196L-asparagine+growth
2326817561L-cysteine+growth
2326818287L-fucose+growth
2326829985L-glutamate+growth
2326818050L-glutamine+growth
2326815971L-histidine+growth
2326817191L-isoleucine+growth
2326818019L-lysine+growth
2326817203L-proline+growth
2326817115L-serine+growth
2326816857L-threonine+growth
2326816414L-valine+growth
2326824996lactate+growth
2326825115malate+growth
2326817790methanol+growth
2326818401phenylacetate+growth
2326817272propionate+growth
232689300suberic acid+growth
2326830031succinate+growth
2326831011valerate+growth
232684853esculin+hydrolysis
2326817632nitrate+reduction
2326816301nitrite+reduction

metabolite production

@refChebi-IDmetaboliteproduction
2326835581indoleno
2326816136hydrogen sulfideno
2326815688acetoinyes

metabolite tests

  • @ref: 23268
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
23268arginine dihydrolase-3.5.3.6
23268beta-galactosidase-3.2.1.23
23268catalase-1.11.1.6
23268cytochrome oxidase+1.9.3.1
23268lipase-
23268lysine decarboxylase-4.1.1.18
23268ornithine decarboxylase-4.1.1.17
23268tryptophan deaminase-4.1.99.1
23268urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16223sludge of the aerobic treatment tanks of a municipal leachate treatment plantDaejeonRepublic of KoreaKORAsia
67771From aerobic sludgeRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Engineered#Waste#Water treatment plant
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4888.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_739;97_862;98_3660;99_4888&stattab=map
  • Last taxonomy: Castellaniella caeni subclade
  • 16S sequence: AB166879
  • Sequence Identity:
  • Total samples: 966
  • soil counts: 323
  • aquatic counts: 494
  • animal counts: 107
  • plant counts: 42

Safety information

risk assessment

  • @ref: 16223
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16223
  • description: Castellaniella caeni gene for 16S rRNA, partial sequence
  • accession: AB166879
  • length: 1452
  • database: ena
  • NCBI tax ID: 266123

GC content

@refGC-contentmethod
1622363.5
2326863.5high performance liquid chromatography (HPLC)
6777165.2high performance liquid chromatography (HPLC)

External links

@ref: 16223

culture collection no.: DSM 22240, KCTC 12197, LMG 23411

straininfo link

  • @ref: 70029
  • straininfo: 232738

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18768620Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant.Liu QM, Ten LN, Im WT, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.65314-02008Alcaligenaceae/*classification/genetics/*isolation & purification/physiology, Anaerobiosis, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Industrial Microbiology, Locomotion, Molecular Sequence Data, Nitrogen/metabolism, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sewage/*microbiology, Ubiquinone/analysisGenetics
Phylogeny19605728Castellaniella ginsengisoli sp. nov., a beta-glucosidase-producing bacterium.Kim MK, Srinivasan S, Kim YJ, Yang DCInt J Syst Evol Microbiol10.1099/ijs.0.008300-02009Alcaligenaceae/*classification/enzymology/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Korea, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Panax, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analysis, beta-Glucosidase/*biosynthesisEnzymology
Phylogeny19819992Castellaniella daejeonensis sp. nov., isolated from soil.Lee M, Jung HM, Woo SG, Yoo SA, Ten LNInt J Syst Evol Microbiol10.1099/ijs.0.018887-02009Alcaligenaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyMetabolism
Phylogeny22523161Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana.Glaeser SP, Galatis H, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.041392-02012Alcaligenaceae/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Leeches/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Putrescine/analogs & derivatives/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analysisGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16223Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22240)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22240
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23268Qing-Mei Liu, Leonid N. Ten, Wan-Taek Im, Sung-Taik Lee10.1099/ijs.0.65314-0Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plantIJSEM 58: 2141-2146 200818768620
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70029Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID232738.1StrainInfo: A central database for resolving microbial strain identifiers