Strain identifier

BacDive ID: 347

Type strain: No

Species: Castellaniella defragrans

Strain Designation: 62Car

Strain history: CIP <- 1998, J. Harder, Max Planck Inst., Bremen, Germany: strain 62Car, Alcaligenes defragrans

NCBI tax ID(s): 75697 (species)

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General

@ref: 4618

BacDive-ID: 347

DSM-Number: 12142

keywords: Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Castellaniella defragrans 62Car is an aerobe, mesophilic, Gram-negative bacterium that was isolated from ditch in a forest.

NCBI tax id

  • NCBI tax id: 75697
  • Matching level: species

strain history

@refhistory
4618<- J. Harder; 62Car
118874CIP <- 1998, J. Harder, Max Planck Inst., Bremen, Germany: strain 62Car, Alcaligenes defragrans

doi: 10.13145/bacdive347.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Alcaligenaceae
  • genus: Castellaniella
  • species: Castellaniella defragrans
  • full scientific name: Castellaniella defragrans (Foss et al. 1998) Kämpfer et al. 2006

  • synonyms

    • @ref: 20215
    • synonym: Alcaligenes defragrans

@ref: 4618

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Alcaligenaceae

genus: Castellaniella

species: Castellaniella defragrans

full scientific name: Castellaniella defragrans (Foss et al. 1998) Kämpfer et al. 2006

strain designation: 62Car

type strain: no

Morphology

cell morphology

  • @ref: 118874
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118874

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4618ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315)yeshttps://mediadive.dsmz.de/medium/1315Name: ANAEROBIC THAUERA MEDIUM (DSMZ Medium 1315) Composition: NaHCO3 2.52 g/l Na2HPO4 x 2 H2O 1.45 g/l K-acetate 0.98 g/l KNO3 0.85 g/l MgSO4 x 7 H2O 0.5 g/l NH4Cl 0.3 g/l KH2PO4 0.25 g/l NaCl 0.05 g/l MnSO4 0.01 g/l CaCl2 0.01 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l NaOH 0.001 g/l alpha-lipoic acid 0.00025 g/l Riboflavin 0.00025 g/l Thiamine-HCl x 2 H2O 0.00025 g/l Vitamin B12 0.00025 g/l Pantothenic acid 0.00025 g/l p-Aminobenzoic acid 0.00025 g/l CoCl2 x 6 H2O 0.00019 g/l Nicotinic acid 0.000125 g/l Nicotine amide 0.000125 g/l Folic acid 0.0001 g/l MnCl2 x 4 H2O 0.0001 g/l Biotin 0.0001 g/l ZnCl2 7e-05 g/l Pyridoxamine hydrochloride 5e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l Na2WO4 x 2 H2O 8e-06 g/l Na2SeO3 x 5 H2O 6e-06 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
4618REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yeshttps://mediadive.dsmz.de/medium/1aName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
36189MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
118874CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4618positivegrowth30mesophilic
36189positivegrowth30mesophilic
54934positivegrowth25mesophilic
118874positivegrowth15-37
118874nogrowth5psychrophilic
118874nogrowth41thermophilic
118874nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4618aerobe
4618anaerobe
54934aerobe
118874obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
118874NaClpositivegrowth0-4 %
118874NaClnogrowth6 %
118874NaClnogrowth8 %
118874NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11887416947citrate+carbon source
1188744853esculin-hydrolysis
118874606565hippurate+hydrolysis
11887417632nitrate+builds gas from
11887417632nitrate+reduction
11887416301nitrite+builds gas from
11887416301nitrite+reduction
11887415792malonate-assimilation
11887417632nitrate+respiration

antibiotic resistance

  • @ref: 118874
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 118874
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11887415688acetoin-
11887417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
118874oxidase+
118874beta-galactosidase-3.2.1.23
118874alcohol dehydrogenase-1.1.1.1
118874gelatinase+
118874amylase-
118874DNase-
118874caseinase-3.4.21.50
118874catalase+1.11.1.6
118874tween esterase-
118874gamma-glutamyltransferase+2.3.2.2
118874lecithinase-
118874lipase-
118874lysine decarboxylase-4.1.1.18
118874ornithine decarboxylase-4.1.1.17
118874phenylalanine ammonia-lyase+4.3.1.24
118874protease-
118874tryptophan deaminase-
118874urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118874--++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118874-------------------------------+-----------------++--+++--+---++++++--+--+-+---++-------+++++++-+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
4618ditch in a forestGermanyDEUEurope
54934Ditch in a forestGermanyDEUEurope1995
118874Environment, Forest ditchGermanyDEUEuropeNorthern1995

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Forest

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
46181Risk group (German classification)
1188741Risk group (French classification)

External links

@ref: 4618

culture collection no.: DSM 12142, CCUG 39791, CIP 105604

straininfo link

  • @ref: 70027
  • straininfo: 12164

Reference

@idauthorscataloguedoi/urltitle
4618Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12142)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12142
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36189Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17641
54934Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 39791)https://www.ccug.se/strain?id=39791
68382Automatically annotated from API zym
70027Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12164.1StrainInfo: A central database for resolving microbial strain identifiers
118874Curators of the CIPCollection of Institut Pasteur (CIP 105604)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105604