Strain identifier

BacDive ID: 3246

Type strain: Yes

Species: Aquicella lusitana

Strain Designation: SGT-39

Strain history: CIP <- 2002, Milton S. da Costa, Coimbra, Portugal: SGT-39

NCBI tax ID(s): 254246 (species)

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General

@ref: 6377

BacDive-ID: 3246

DSM-Number: 16500

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Aquicella lusitana SGT-39 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water at the spa.

NCBI tax id

  • NCBI tax id: 254246
  • Matching level: species

strain history

@refhistory
6377<- CIP <- M. S. da Costa; SGT-39
120679CIP <- 2002, Milton S. da Costa, Coimbra, Portugal: SGT-39

doi: 10.13145/bacdive3246.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Coxiellaceae
  • genus: Aquicella
  • species: Aquicella lusitana
  • full scientific name: Aquicella lusitana Santos et al. 2004

@ref: 6377

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Coxiellaceae

genus: Aquicella

species: Aquicella lusitana

full scientific name: Aquicella lusitana Santos et al. 2004

strain designation: SGT-39

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.99
120679negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6377BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
39914MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
120679CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
6377positivegrowth37mesophilic
39914positivegrowth37mesophilic
58377positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 58377
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.837

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120679oxidase-
120679catalase-1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120679--++-+----+---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6377water at the spaSao GemilPortugalPRTEurope
58377Water,borehole at spaPortugalPRTEurope
120679Environment, Borehole water at spaPortugalPRTEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
63771Risk group (German classification)
1206791Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Aquicella lusitana strain SGT-39T 16S ribosomal RNA gene, partial sequence
  • accession: AY359282
  • length: 1482
  • database: ena
  • NCBI tax ID: 254246

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aquicella lusitana SGT-39GCA_902459475completencbi254246
66792Aquicella lusitana DSM 16500GCA_003350455scaffoldncbi254246
66792Aquicella lusitana strain DSM 16500254246.3wgspatric254246
66792Aquicella lusitana strain SGT-39254246.4completepatric254246
66792Aquicella lusitana DSM 165002756170232draftimg254246

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno81.914no
gram-positiveno98.898no
anaerobicno97.986yes
aerobicno93.283yes
halophileno81.337no
spore-formingno96.21no
thermophileno82.692no
glucose-utilyes66.926no
motileyes75.273no
glucose-fermentno87.814no

External links

@ref: 6377

culture collection no.: DSM 16500, CIP 107650, LMG 21647, CCUG 48880

straininfo link

  • @ref: 72759
  • straininfo: 88961

literature

  • topic: Phylogeny
  • Pubmed-ID: 14602611
  • title: Gamma-proteobacteria Aquicella lusitana gen. nov., sp. nov., and Aquicella siphonis sp. nov. infect protozoa and require activated charcoal for growth in laboratory media.
  • authors: Santos P, Pinhal I, Rainey FA, Empadinhas N, Costa J, Fields B, Benson R, Verissimo A, Da Costa MS
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/AEM.69.11.6533-6540.2003
  • year: 2003
  • mesh: Animals, Bacterial Typing Techniques, Charcoal/*metabolism, Coxiellaceae/classification/genetics, Culture Media, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Gammaproteobacteria/*classification/genetics/growth & development/*physiology, Hartmannella/*microbiology, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, U937 Cells
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6377Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16500)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16500
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39914Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5109
58377Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48880)https://www.ccug.se/strain?id=48880
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72759Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88961.1StrainInfo: A central database for resolving microbial strain identifiers
120679Curators of the CIPCollection of Institut Pasteur (CIP 107650)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107650