Strain identifier

BacDive ID: 3227

Type strain: Yes

Species: Corynebacterium hansenii

Strain Designation: C-138

Strain history: CIP <- 2004, F. Renaud, ISPB, Lyon, France <- A. Le Coustumier, Cahors Hosp., Cahors, France: strain C-138

NCBI tax ID(s): 394964 (species)

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General

@ref: 12351

BacDive-ID: 3227

DSM-Number: 45109

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Corynebacterium hansenii C-138 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from human liposarcoma of an 77-year-old patient.

NCBI tax id

  • NCBI tax id: 394964
  • Matching level: species

strain history

@refhistory
12351<- CCUG <- F. N. R. Renaud, Univ.Claude Bernard, Lyon; C-138
67770CCUG 53252 <-- F. N. R. Renaud C-138.
119835CIP <- 2004, F. Renaud, ISPB, Lyon, France <- A. Le Coustumier, Cahors Hosp., Cahors, France: strain C-138

doi: 10.13145/bacdive3227.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium hansenii
  • full scientific name: Corynebacterium hansenii Renaud et al. 2007

@ref: 12351

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium hansenii

full scientific name: Corynebacterium hansenii Renaud et al. 2007

strain designation: C-138

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
31927positiverod-shapedno
119835positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
19916Beige10-14 daysISP 2
19916Beige10-14 daysISP 3
19916Beige10-14 daysISP 4
19916Beige10-14 daysISP 5
19916Beige10-14 daysISP 6
19916Beige10-14 daysISP 7
119835

multicellular morphology

@refforms multicellular complexmedium name
19916noISP 2
19916noISP 3
19916noISP 4
19916noISP 5
19916noISP 6
19916noISP 7

pigmentation

  • @ref: 31927
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12351COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
12351TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19916ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19916ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19916ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19916ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19916ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19916ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33242MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119835CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
19916positiveoptimum28mesophilic
12351positivegrowth37mesophilic
31927positiveoptimum20psychrophilic
33242positivegrowth37mesophilic
67770positivegrowth37mesophilic
119835positivegrowth25-45
119835nogrowth10psychrophilic
119835nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31927aerobe
119835obligate aerobe

spore formation

  • @ref: 31927
  • spore formation: no

halophily

  • @ref: 119835
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3192728757fructose+carbon source
3192728260galactose+carbon source
3192717234glucose+carbon source
3192733942ribose+carbon source
3192717992sucrose+carbon source
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11983516947citrate-carbon source
1198354853esculin-hydrolysis
119835606565hippurate+hydrolysis
11983517632nitrate-reduction
11983516301nitrite-reduction
11983545285pyrazinamide+hydrolysis
11983517632nitrate-respiration

antibiotic resistance

  • @ref: 119835
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 119835
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11983515688acetoin-
11983517234glucose-

enzymes

@refvalueactivityec
31927catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382alkaline phosphatase-3.1.3.1
119835oxidase-
119835beta-galactosidase+3.2.1.23
119835alcohol dehydrogenase-1.1.1.1
119835gelatinase-
119835amylase+
119835DNase-
119835caseinase-3.4.21.50
119835catalase+1.11.1.6
119835tween esterase-
119835gamma-glutamyltransferase-2.3.2.2
119835lecithinase-
119835lipase-
119835lysine decarboxylase-4.1.1.18
119835ornithine decarboxylase-4.1.1.17
119835phenylalanine ammonia-lyase-4.3.1.24
119835tryptophan deaminase-
119835urease+3.5.1.5

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19916-----+-+------+----
119835-++++---------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119835----+----++++--------------+/---++----------------+/-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119835+++++--+-+---------+-----------------------------+---------+---------------+---++-------+-+--------

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentisolation date
12351human liposarcoma of an 77-year-old patient
59846Human liposarcoma,77-yr-old patient2004-03-01CahorsFranceFRAEurope
67770Human liposarcoma pus
119835Liposarcoma infectionCahorsFranceFRAEurope2004

isolation source categories

Cat1Cat2
#Infection#Patient
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_3755.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1038;97_1212;98_1453;99_3755&stattab=map
  • Last taxonomy: Corynebacterium
  • 16S sequence: AM946639
  • Sequence Identity:
  • Total samples: 1611
  • soil counts: 109
  • aquatic counts: 47
  • animal counts: 1432
  • plant counts: 23

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
12351yes, in single cases1Risk group (German classification)
199162Risk group (German classification)
1198352Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium hansenii partial 16S rRNA gene, type strain DSM 45109TAM9466391490ena394964
12351Corynebacterium hansenii strain C-138 16S ribosomal RNA gene, partial sequenceAY684044937ena394964

External links

@ref: 12351

culture collection no.: JCM 15293, DSM 45109, CCUG 53252, CIP 108444

straininfo link

  • @ref: 72741
  • straininfo: 303322

literature

  • topic: Phylogeny
  • Pubmed-ID: 17473268
  • title: Corynebacterium hansenii sp. nov., an alpha-glucosidase-negative bacterium related to Corynebacterium xerosis.
  • authors: Renaud FNR, Coustumier AL, Wilhem N, Aubel D, Riegel P, Bollet C, Freney J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64665-0
  • year: 2007
  • mesh: Aged, Bacterial Proteins/genetics, Carbon/metabolism, Corynebacterium/*classification/*isolation & purification, Corynebacterium Infections/microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/genetics, DNA-Directed RNA Polymerases/genetics, Fermentation, Genes, rRNA, Humans, Liposarcoma/complications, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, alpha-Glucosidases/*biosynthesis
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
12351Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45109)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45109
19916Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45109.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
31927Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128184
33242Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6067
59846Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 53252)https://www.ccug.se/strain?id=53252
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72741Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID303322.1StrainInfo: A central database for resolving microbial strain identifiers
119835Curators of the CIPCollection of Institut Pasteur (CIP 108444)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108444