Strain identifier

BacDive ID: 3225

Type strain: Yes

Species: Corynebacterium tuscaniense

Strain Designation: ISS-5309

Strain history: CIP <- 2006, CCUG <- 2005, R. Creti, R. Mattei, C. von Hunolstein, Rome, Italy: strain ISS-5309

NCBI tax ID(s): 302449 (species)

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General

@ref: 12343

BacDive-ID: 3225

DSM-Number: 45101

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Corynebacterium tuscaniense ISS-5309 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from human blood of a 85 year old woman.

NCBI tax id

  • NCBI tax id: 302449
  • Matching level: species

strain history

@refhistory
12343<- CCUG <- R. Creti, R. Mattei, C. von Hunolstein
67770CCUG 51321 <-- R. Creti et al. ISS-5309.
123699CIP <- 2006, CCUG <- 2005, R. Creti, R. Mattei, C. von Hunolstein, Rome, Italy: strain ISS-5309

doi: 10.13145/bacdive3225.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium tuscaniense
  • full scientific name: Corynebacterium tuscaniense corrig. Riegel et al. 2006

  • synonyms

    • @ref: 20215
    • synonym: Corynebacterium tuscaniae

@ref: 12343

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium tuscaniense

full scientific name: Corynebacterium tuscaniense Riegel et al. 2006

strain designation: ISS-5309

type strain: yes

Morphology

cell morphology

  • @ref: 123699
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

multimedia

  • @ref: 12343
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45101.jpg
  • caption: Medium 693 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12343COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
12343TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
37539MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123699CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
12343positivegrowth37mesophilic
37539positivegrowth37mesophilic
59146positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59146aerobe
123699obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123699nitrate-reduction17632
123699nitrite-reduction16301

metabolite production

  • @ref: 123699
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
123699oxidase-
123699catalase+1.11.1.6
123699urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123699-+++-+----+----+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123699---------+/-+----------------+---------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
12343human blood of a 85 year old womanItalyITAEurope
59146Human blood,85-yr-old woman,endocarditisItalyITAEurope2003-06-01Lucca
67770Blood culture from a patient with infective endocarditis
123699Human, BloodItalyITAEurope2003

isolation source categories

Cat1Cat2Cat3
#Host#Human#Female
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_697.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_386;97_489;98_560;99_697&stattab=map
  • Last taxonomy: Corynebacterium tuscaniense
  • 16S sequence: AY677186
  • Sequence Identity:
  • Total samples: 1956
  • soil counts: 4
  • aquatic counts: 40
  • animal counts: 1908
  • plant counts: 4

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
12343yes, in single cases1Risk group (German classification)
1236992Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Corynebacterium tuscaniense strain ISS-5309 16S ribosomal RNA gene, partial sequence
  • accession: AY677186
  • length: 1502
  • database: ena
  • NCBI tax ID: 302449

Genome sequences

  • @ref: 67770
  • description: Corynebacterium tuscaniense CCUG 51321T
  • accession: GCA_008693065
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 302449

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno92.599no
flagellatedno97.537no
gram-positiveyes93.601no
anaerobicno98.053no
aerobicno75.362no
halophileyes73.052no
spore-formingno93.918no
thermophileno93.804yes
glucose-utilyes83.583no
glucose-fermentno69.078no

External links

@ref: 12343

culture collection no.: JCM 15294, DSM 45101, ATCC BAA 1141, CCUG 51321, CIP 109389

straininfo link

  • @ref: 72739
  • straininfo: 297168

literature

  • topic: Phylogeny
  • Pubmed-ID: 16455875
  • title: Isolation of Corynebacterium tuscaniae sp. nov. from blood cultures of a patient with endocarditis.
  • authors: Riegel P, Creti R, Mattei R, Nieri A, von Hunolstein C
  • journal: J Clin Microbiol
  • DOI: 10.1128/JCM.44.2.307-312.2006
  • year: 2006
  • mesh: Aged, 80 and over, Bacterial Typing Techniques, Blood/*microbiology, Corynebacterium/*classification/genetics/*isolation & purification, Corynebacterium Infections/*microbiology, *Culture Media, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Endocarditis, Bacterial/*microbiology, Female, Genes, rRNA, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Cultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12343Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45101)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45101
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37539Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7124
59146Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51321)https://www.ccug.se/strain?id=51321
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72739Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297168.1StrainInfo: A central database for resolving microbial strain identifiers
123699Curators of the CIPCollection of Institut Pasteur (CIP 109389)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109389