Strain identifier

BacDive ID: 3088

Type strain: No

Species: Corynebacterium glutamicum

Strain history: K. Suzuki CNF 015 <-- AJ 1517 <-- NRRL B-2243 <-- Int. Miner. & Chem. Corp..

NCBI tax ID(s): 1718 (species)

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General

@ref: 8562

BacDive-ID: 3088

DSM-Number: 20137

keywords: 16S sequence, Bacteria, mesophilic, amino acid production

description: Corynebacterium glutamicum DSM 20137 is a mesophilic bacterium that produces amino acids.

NCBI tax id

  • NCBI tax id: 1718
  • Matching level: species

strain history

@refhistory
8562<- ATCC <- NRRL <- Int. Mineral and Chemical Corp
67770K. Suzuki CNF 015 <-- AJ 1517 <-- NRRL B-2243 <-- Int. Miner. & Chem. Corp..

doi: 10.13145/bacdive3088.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium glutamicum
  • full scientific name: Corynebacterium glutamicum (Kinoshita et al. 1958) Abe et al. 1967 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Micrococcus glutamicus
    20215Brevibacterium divaricatum
    20215Corynebacterium lilium

@ref: 8562

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium glutamicum

full scientific name: Corynebacterium glutamicum (Kinoshita et al. 1958) Abe et al. 1967 emend. Nouioui et al. 2018

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8562
  • name: CORYNEBACTERIUM AGAR (DSMZ Medium 53)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/53
  • composition: Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
8562positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

compound production

@refcompound
8562amino acids
8562L glutamic acid
20216L-glutamic acid

murein

  • @ref: 8562
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 67770
  • Chebi-ID: 29985
  • metabolite: L-glutamate
  • production: yes

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYG
8562+--------+--++--+-+-

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
8562-----+--------------

Safety information

risk assessment

  • @ref: 8562
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Corynebacterium glutamicum DSM 20137 16S ribosomal RNA gene, partial sequence
  • accession: AY017109
  • length: 632
  • database: ena
  • NCBI tax ID: 1718

GC content

@refGC-contentmethod
856253.0thermal denaturation, midpoint method (Tm)
856252.9
6777056.8
6777052.9thermal denaturation, midpoint method (Tm)

External links

@ref: 8562

culture collection no.: DSM 20137, ATCC 15990, NCIB 10337, NRRL B-2243, JCM 1321, BCRC 11669, CGMCC 1.1888, IAM 12434, IMET 11081, KCTC 1448, LMG 3654, NCIMB 10337

straininfo link

  • @ref: 72606
  • straininfo: 2071

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1713055Transfer of Brevibacterium divaricatum DSM 20297T, "Brevibacterium flavum" DSM 20411, "Brevibacterium lactofermentum" DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns.Liebl W, Ehrmann M, Ludwig W, Schleifer KHInt J Syst Bacteriol10.1099/00207713-41-2-2551991Base Composition, Base Sequence, Brevibacterium/*classification/genetics, Corynebacterium/*classification/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, *Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*genetics, RNA, Ribosomal, 23S/*geneticsGenetics
Metabolism2283029Isolation and characterization of a restriction and modification deficient mutant of Brevibacterium lactofermentum.Bonnassie S, Oreglia J, Trautwetter A, Sicard AMFEMS Microbiol Lett10.1016/0378-1097(90)90361-s1990Bacteriophages/genetics/growth & development, Brevibacterium/*genetics, Corynebacterium/metabolism, DNA, Viral/metabolism, *Mutation, Nitrosoguanidines/pharmacology, *Transfection, *Transformation, Bacterial, Viral Plaque AssayPhylogeny
Enzymology3449603Characterization of the corynebacteriophage CG33.Trautwetter A, Blanco C, Bonnassie SJ Gen Microbiol10.1099/00221287-133-10-29451987Bacteriophages/genetics/*isolation & purification/ultrastructure, Corynebacterium/*analysis, DNA, Viral, Genes, Viral, Microscopy, Electron, Virus ReplicationPhylogeny
Genetics7961503Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli.Schafer A, Schwarzer A, Kalinowski J, Puhler AJ Bacteriol10.1128/jb.176.23.7309-7319.19941994Adaptation, Biological, Amino Acid Sequence, Base Sequence, Conjugation, Genetic/*genetics, Corynebacterium/*genetics, Crosses, Genetic, DNA Mutational Analysis, DNA Restriction-Modification Enzymes/*genetics, DNA, Bacterial/genetics, Escherichia coli/*genetics, Genetic Complementation Test, Genomic Library, Molecular Sequence Data, Open Reading Frames/genetics, Plasmids/genetics, Restriction Mapping, Sequence Analysis, DNA, Species SpecificityEnzymology
Metabolism23548274Impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: evidence of an atypical metabolism in Bacillus megaterium DSM 509.Shahid S, Mosrati R, Ledauphin J, Amiel C, Fontaine P, Gaillard JL, Corroler DJ Biosci Bioeng10.1016/j.jbiosc.2013.02.0172013Bacillus megaterium/classification/growth & development/*metabolism, Carbon/*metabolism, Corynebacterium glutamicum/metabolism, Nitrogen/*metabolism, Polyhydroxyalkanoates/*biosynthesis, Pseudomonas putida/metabolismPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8562Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20137)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20137
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
72606Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2071.1StrainInfo: A central database for resolving microbial strain identifiers