Strain identifier

BacDive ID: 3065

Type strain: Yes

Species: Slackia faecicanis

Strain Designation: 5WC12

Strain history: CIP <- 2004, CCUG <- Lawson, UK: strain 5WC12

NCBI tax ID(s): 255723 (species)

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General

@ref: 7050

BacDive-ID: 3065

DSM-Number: 17537

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Slackia faecicanis 5WC12 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from faeces, dog.

NCBI tax id

  • NCBI tax id: 255723
  • Matching level: species

strain history

@refhistory
7050<- CIP <- P. Lawson; 5WC12
67770CIP 108281 <-- CCUG 48399 <-- P. A. Lawson 5WC12.
119220CIP <- 2004, CCUG <- Lawson, UK: strain 5WC12

doi: 10.13145/bacdive3065.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Coriobacteriia
  • order: Eggerthellales
  • family: Eggerthellaceae
  • genus: Slackia
  • species: Slackia faecicanis
  • full scientific name: Slackia faecicanis Lawson et al. 2005

@ref: 7050

domain: Bacteria

phylum: Actinobacteria

class: Coriobacteriia

order: Coriobacteriales

family: Eggerthellaceae

genus: Slackia

species: Slackia faecicanis

full scientific name: Slackia faecicanis Lawson et al. 2005

strain designation: 5WC12

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapeconfidencemotility
31432positive1.5 µm0.5 µmrod-shaped
69480positive99.998
119220positiverod-shapedno

colony morphology

  • @ref: 119220

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7050PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
33220MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
119220CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
7050positivegrowth37mesophilic
31432positiveoptimum37mesophilic
33220positivegrowth37mesophilic
58133positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
7050anaerobe
31432anaerobe
58133anaerobe
69480anaerobe99.579
119220anaerobe

spore formation

@refspore formationconfidence
31432no
69481no100
69480no99.937

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11922017108D-arabinose-degradation
11922015824D-fructose-degradation
11922017634D-glucose-degradation
11922065327D-xylose-degradation
11922017057cellobiose-degradation
11922017716lactose-degradation
11922017306maltose-degradation
11922017814salicin-degradation
11922017992sucrose-degradation
1192204853esculin-hydrolysis
11922017632nitrate-reduction
11922016301nitrite-reduction
11922017632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838027897tryptophan-energy source
6838129016arginine+hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
11922035581indoleno
6838035581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin-
6838035581indole-

enzymes

@refvalueactivityec
31432acid phosphatase+3.1.3.2
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease+3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
119220oxidase-
119220beta-galactosidase-3.2.1.23
119220gelatinase-
119220catalase-1.11.1.6
119220urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
58133--++----------------
119220--+-----------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
7050-+---------------------------
58133-+------------+--------------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
58133+------------------------------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
7050faeces, dogUnited KingdomGBREurope
58133Canine feces,healthy male Labrador dogUnited KingdomGBREuropeReading ?
67770Canine feces
119220Animal, Dog

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_21783.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_264;96_11016;97_13346;98_16428;99_21783&stattab=map
  • Last taxonomy: Slackia faecicanis subclade
  • 16S sequence: AJ608686
  • Sequence Identity:
  • Total samples: 10486
  • soil counts: 67
  • aquatic counts: 161
  • animal counts: 10248
  • plant counts: 10

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
70501Risk group (German classification)
1192201Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7050
  • description: Slackia faecicanis partial 16S rRNA gene, type strain CCUG 48399
  • accession: AJ608686
  • length: 1476
  • database: ena
  • NCBI tax ID: 255723

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Slackia faecicanis strain DSM 17537255723.3wgspatric255723
66792Slackia faecicanis DSM 175372866339652draftimg255723
67770Slackia faecicanis DSM 17537GCA_003725295contigncbi255723

GC content

@refGC-contentmethod
705061.2
6777061.2high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.501no
gram-positiveyes94.012yes
anaerobicyes98.882yes
aerobicno97.879yes
halophileno82.141no
spore-formingno94.108no
glucose-utilyes66.49no
flagellatedno98.496no
thermophileno92.548yes
glucose-fermentyes51.689no

External links

@ref: 7050

culture collection no.: DSM 17537, CCUG 48399, CIP 108281, JCM 14555

straininfo link

  • @ref: 72585
  • straininfo: 138302

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15879263Slackia faecicanis sp. nov., isolated from canine faeces.Lawson PA, Greetham HL, Gibson GR, Giffard C, Falsen E, Collins MDInt J Syst Evol Microbiol10.1099/ijs.0.63531-02005Actinobacteria/*classification/cytology/*isolation & purification/physiology, Anaerobiosis, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Dogs/*microbiology, Feces/*microbiology, Genes, Bacterial, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAEnzymology
Genetics30643901Draft Genome Sequences of Type Strains of Gordonibacter faecihominis, Paraeggerthella hongkongensis, Parvibacter caecicola,Slackia equolifaciens, Slackia faecicanis, and Slackia isoflavoniconvertens.Danylec N, Stoll DA, Dotsch A, Huch MMicrobiol Resour Announc10.1128/MRA.01532-182019

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7050Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17537)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17537
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31432Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2773828776041
33220Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5886
58133Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48399)https://www.ccug.se/strain?id=48399
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72585Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID138302.1StrainInfo: A central database for resolving microbial strain identifiers
119220Curators of the CIPCollection of Institut Pasteur (CIP 108281)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108281