Strain identifier

BacDive ID: 2997

Type strain: No

Species: Simplicispira metamorpha

Strain Designation: D-416

Strain history: CIP <- 2007, DSMZ <- G.A. Dubinina: strain D-416

NCBI tax ID(s): 80881 (species)

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General

@ref: 4841

BacDive-ID: 2997

DSM-Number: 12826

keywords: Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped

description: Simplicispira metamorpha D-416 is an obligate aerobe, Gram-negative, motile bacterium that was isolated from active sludge of waste water aeration tank.

NCBI tax id

  • NCBI tax id: 80881
  • Matching level: species

strain history

@refhistory
4841<- G. A. Dubinina; D-416 (Aquaspirillum sp.)
120922CIP <- 2007, DSMZ <- G.A. Dubinina: strain D-416

doi: 10.13145/bacdive2997.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Simplicispira
  • species: Simplicispira metamorpha
  • full scientific name: Simplicispira metamorpha (Terasaki 1961) Grabovich et al. 2006

  • synonyms

    @refsynonym
    20215Spirillum metamorphum
    20215Aquaspirillum metamorphum

@ref: 4841

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Simplicispira

species: Simplicispira metamorpha

full scientific name: Simplicispira metamorpha (Terasaki 1961) Grabovich et al. 2006

strain designation: D-416

type strain: no

Morphology

cell morphology

  • @ref: 120922
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36812MEDIUM 65 - for Prolinoborus fasciculusyesDistilled water make up to (1000.000 ml);Manganese II sulphate monohydrate(2.000 mg);Magnesium sulphate heptahydrate (1.000 g);Agar (15.000 g);Ammonium sulphate (1.000 g);Peptone (10.000 g);Succinic acid (1.680 g);Ferric III chloride hexahydrate (2.000mg)
4841SPIRILLUM MEDIUM (DSMZ Medium 37)yesName: SPIRILLUM MEDIUM (DSMZ Medium 37) Composition: Peptone 10.0 g/l (NH4)2SO4 1.0 g/l Succinic acid 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeCl3 x 6 H2O 0.002 g/l MnSO4 x H2O 0.002 g/l Distilled waterhttps://mediadive.dsmz.de/medium/37
4841AQUASPIRILLUM MEDIUM (DSMZ Medium 888)yesName: AQUASPIRILLUM MEDIUM (DSMZ Medium 888) Composition: Casamino acids 1.5 g/l Sodium succinate 1.0 g/l (NH4)2SO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l Agar 0.5 g/l Na2S2O3 x 5 H2O 0.1 g/l CaCl2 x 6 H2O 0.03 g/l Na2HPO4 0.01 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.0022 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l H3BO3 3e-05 g/l NiCl2 x 6 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l CuCl2 x 2 H2O 3e-05 g/l MnCl2 x 4 H2O 3e-05 g/l Pyridoxine 2.5e-05 g/l Riboflavin 1.25e-05 g/l Nicotinic acid 1.25e-05 g/l PABA 1.25e-05 g/l Calcium pantothenate 1.25e-05 g/l Thiamine 1.25e-05 g/l Biotin 5e-06 g/l Folic acid 5e-06 g/l B12 1.25e-06 g/l NaOH Distilled waterhttps://mediadive.dsmz.de/medium/888
120922CIP Medium 65yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=65
120922CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
4841positivegrowth28
36812positivegrowth30

Physiology and metabolism

oxygen tolerance

  • @ref: 120922
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12092217632nitrate-reduction
12092216301nitrite-reduction

metabolite production

  • @ref: 120922
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120922oxidase+
120922catalase+1.11.1.6
120922urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120922-+++-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4841active sludge of waste water aeration tankJaroslave regionRussiaRUSEurope
120922Environment, Active sludge of waste water aeration tankRussian FederationRUSEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Engineered#Waste#Activated sludge
#Engineered#Waste#Wastewater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
48411Risk group (German classification)
1209221Risk group (French classification)

External links

@ref: 4841

culture collection no.: DSM 12826, CIP 109450

straininfo link

  • @ref: 72519
  • straininfo: 49374

literature

  • topic: Phylogeny
  • Pubmed-ID: 16514029
  • title: Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov., and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov.
  • authors: Grabovich M, Gavrish E, Kuever J, Lysenko AM, Podkopaeva D, Dubinina G
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64027-0
  • year: 2006
  • mesh: Comamonadaceae/*classification/cytology/genetics/physiology, DNA, Ribosomal/chemistry, Industrial Waste, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4841Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12826)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12826
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36812Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7195
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
72519Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49374.1StrainInfo: A central database for resolving microbial strain identifiers
120922Curators of the CIPCollection of Institut Pasteur (CIP 109450)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109450