Strain identifier

BacDive ID: 2993

Type strain: No

Species: Roseateles depolymerans

Strain Designation: 61B2

Strain history: <- T. Suyama; 61B2

NCBI tax ID(s): 76731 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4501

BacDive-ID: 2993

DSM-Number: 11814

keywords: 16S sequence, Bacteria, aerobe, mesophilic

description: Roseateles depolymerans 61B2 is an aerobe, mesophilic bacterium that was isolated from fresh water.

NCBI tax id

  • NCBI tax id: 76731
  • Matching level: species

strain history

  • @ref: 4501
  • history: <- T. Suyama; 61B2

doi: 10.13145/bacdive2993.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Roseateles
  • species: Roseateles depolymerans
  • full scientific name: Roseateles depolymerans Suyama et al. 1999

@ref: 4501

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Roseateles

species: Roseateles depolymerans

full scientific name: Roseateles depolymerans Suyama et al. 1999

strain designation: 61B2

type strain: no

Morphology

colony morphology

  • @ref: 59471
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4501HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428)yeshttps://mediadive.dsmz.de/medium/428Name: HETEROTROPHIC MEDIUM H3P (DSMZ Medium 428) Composition: Agar 19.802 g/l Na2HPO4 x 2 H2O 2.87129 g/l KH2PO4 2.27723 g/l D-Glucose 1.9802 g/l Na-lactate 0.990099 g/l Na-pyruvate 0.990099 g/l D-Mannitol 0.990099 g/l DL-Malate 0.990098 g/l Na-acetate 0.990098 g/l Yeast extract 0.990098 g/l NH4Cl 0.990098 g/l Disodium succinate 0.990098 g/l MgSO4 x 7 H2O 0.495049 g/l Ferric ammonium citrate 0.049505 g/l CaCl2 x 2 H2O 0.00990098 g/l NaVO3 x H2O 0.00495049 g/l Thiamine-HCl x 2 H2O 0.00247525 g/l Nicotinic acid 0.00247525 g/l Pyridoxine hydrochloride 0.00247525 g/l Calcium pantothenate 0.00247525 g/l H3BO3 0.00148515 g/l CoCl2 x 6 H2O 0.000990099 g/l Riboflavin 0.00049505 g/l ZnSO4 x 7 H2O 0.00049505 g/l MnCl2 x 4 H2O 0.000148515 g/l Na2MoO4 x 2 H2O 0.000148515 g/l NiCl2 x 6 H2O 9.90099e-05 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Vitamin B12 4.95049e-05 g/l Folic acid 9.90099e-06 g/l Biotin 4.9505e-06 g/l Distilled water
4501NUTRIENT AGAR (DSMZ Medium 1)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1.pdf

culture temp

@refgrowthtypetemperaturerange
59471positivegrowth30mesophilic
4501positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
59471aerobe
59471obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    59471C12:00.812
    59471C14:01.114
    59471C15:01.515
    59471C16:028.216
    59471C17:00.917
    59471C18:00.318
    59471C10:0 3OH511.423
    59471C12:0 2OH1.913.178
    59471C14:0 2OH0.515.205
    59471C14:0 3OH/C16:1 ISO I415.485
    59471C14:1 ω5c0.413.899
    59471C15:1 ω6c1.214.856
    59471C16:0 iso0.315.626
    59471C16:1 ω7c41.915.819
    59471C17:1 ω6c0.816.862
    59471C18:1 ω7c /12t/9t10.517.824
    59471C18:1 ω9c0.317.769
    59471C18:2 ω6,9c/C18:0 ANTE0.317.724
    59471Unidentified0.215.271
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
59471-+++-+--+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
59471------+++++++++++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4501fresh waterHanamuro river, Ibaraki prefectureJapanJPNAsia
59471Fresh waterHanamuro riverJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_5856.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_1238;97_1469;98_4356;99_5856&stattab=map
  • Last taxonomy: Roseateles
  • 16S sequence: AB003626
  • Sequence Identity:
  • Total samples: 1380
  • soil counts: 360
  • aquatic counts: 489
  • animal counts: 216
  • plant counts: 315

Safety information

risk assessment

  • @ref: 4501
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Roseateles depolymerans strain 61B2 (DSM11814) gene for 16S rRNA, clone:group1AB0036251452ena76731
20218Roseateles depolymerans strain 61B2 (DSM11814) gene for 16S rRNA, clone:group2AB0036261452ena76731

External links

@ref: 4501

culture collection no.: CCUG 52220, DSM 11814

straininfo link

  • @ref: 72515
  • straininfo: 49186

literature

  • topic: Phylogeny
  • Pubmed-ID: 18175673
  • title: Description of Roseateles aquatilis sp. nov. and Roseateles terrae sp. nov., in the class Betaproteobacteria, and emended description of the genus Roseateles.
  • authors: Gomila M, Bowien B, Falsen E, Moore ER, Lalucat J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65169-0
  • year: 2008
  • mesh: Bacterial Typing Techniques, Comamonadaceae/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/analysis, DNA, Ribosomal Spacer/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4501Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11814)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11814
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
59471Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 52220)https://www.ccug.se/strain?id=52220
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72515Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49186.1StrainInfo: A central database for resolving microbial strain identifiers