Strain identifier

BacDive ID: 2912

Type strain: No

Species: Comamonas testosteroni

Strain history: J. Tamaoka KS 0103 <-- NCIB 8893 <-- S. Dagley.

NCBI tax ID(s): 285 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9 (current version)

General

@ref: 2037

BacDive-ID: 2912

DSM-Number: 38

keywords: 16S sequence, Bacteria, human pathogen

description: Comamonas testosteroni DSM 38 is a human pathogen of the family Comamonadaceae.

NCBI tax id

  • NCBI tax id: 285
  • Matching level: species

strain history

@refhistory
2037<- ATCC <- ? <- S. Dagley
67770J. Tamaoka KS 0103 <-- NCIB 8893 <-- S. Dagley.

doi: 10.13145/bacdive2912.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Comamonas
  • species: Comamonas testosteroni
  • full scientific name: Comamonas testosteroni (Marcus and Talalay 1956) Tamaoka et al. 1987

  • synonyms

    • @ref: 20215
    • synonym: Pseudomonas testosteroni

@ref: 2037

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Comamonas

species: Comamonas testosteroni

full scientific name: Comamonas testosteroni (Marcus and Talalay 1956) Tamaoka et al. 1987 emend. Willems et al. 1991

type strain: no

Culture and growth conditions

culture medium

  • @ref: 2037
  • name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1a
  • composition: Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
2037positivegrowth30
67770positivegrowth30

Physiology and metabolism

compound production

@refcompound
2037alpha hydroxysteroid dehydrogenase
2037ß hydroxysteroid dehydrogenase
2037protocatechuate 4,5-oxygenase

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2037--------------++++--+

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_294.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_202;97_220;98_249;99_294&stattab=map
  • Last taxonomy: Comamonas
  • 16S sequence: LC379085
  • Sequence Identity:
  • Total samples: 45208
  • soil counts: 5571
  • aquatic counts: 17183
  • animal counts: 18603
  • plant counts: 3851

Safety information

risk assessment

  • @ref: 2037
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Comamonas testosteroni gene for 16S rRNA, partial sequence, strain: JCM 6223
  • accession: LC379085
  • length: 1455
  • database: nuccore
  • NCBI tax ID: 285

GC content

  • @ref: 67770
  • GC-content: 61.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 2037

culture collection no.: DSM 38, ATCC 17454, NCIB 8893, JCM 6223, IAM 14426, NCIMB 8893

straininfo link

  • @ref: 72435
  • straininfo: 47043

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology8944761Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni.Oppermann UC, Maser EEur J Biochem10.1111/j.1432-1033.1996.00744.x19963-Hydroxysteroid Dehydrogenases/biosynthesis/immunology/isolation & purification/*metabolism, 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific), Alcohol Oxidoreductases/isolation & purification/metabolism, Aldehyde Reductase, Aldo-Keto Reductases, Amino Acid Sequence, Cross Reactions, Enzyme Induction, Gram-Negative Aerobic Bacteria/drug effects/*enzymology, Molecular Sequence Data, Pseudomonas/enzymology, Sequence Analysis, Subcellular Fractions/enzymology, Substrate Specificity, Testosterone/pharmacology, Xenobiotics/metabolismMetabolism
Enzymology14674583Energization of Comamonas testosteroni ATCC 17454 for indicating toxic effects of chlorophenoxy herbicides.Loffhagen N, Hartig C, Babel WArch Environ Contam Toxicol10.1007/s00244-003-2142-620032,4-Dinitrophenol/*metabolism/pharmacokinetics/toxicity, Adenosine Triphosphate/*metabolism, Alcohol Dehydrogenase/*pharmacology, Coloring Agents/*metabolism/pharmacokinetics/toxicity, Comamonas testosteroni/*enzymology, Electron Transport, Herbicides/*metabolism/pharmacokinetics/toxicity, Hydrogen-Ion Concentration, Inactivation, Metabolic, Oxidation-Reduction, PhosphorylationMetabolism
Metabolism16133339Impact of membrane fatty acid composition on the uncoupling sensitivity of the energy conservation of Comamonas testosteroni ATCC 17454.Loffhagen N, Hartig C, Harms HAppl Microbiol Biotechnol10.1007/s00253-005-0104-92005Cell Membrane/*chemistry, Comamonas testosteroni/*classification/cytology/*metabolism, Culture Media/chemistry, Electron Transport/physiology, Energy Metabolism/*physiology, Fatty Acids/*analysis, Phosphorylation, Pyruvic Acid/chemistry/metabolism, TemperaturePhylogeny

Reference

@idauthorscataloguedoi/urltitle
2037Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 38)https://www.dsmz.de/collection/catalogue/details/culture/DSM-38
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72435Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47043.1StrainInfo: A central database for resolving microbial strain identifiers