Strain identifier

BacDive ID: 286

Type strain: Yes

Species: Aeromonas sanarellii

Strain Designation: A2-67

Strain history: CIP <- 2010, CECT <- M. J. Figueras, Rovira i Virgili Univ., Reus, Spain: strain A2-67

NCBI tax ID(s): 633415 (species)

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General

@ref: 17486

BacDive-ID: 286

DSM-Number: 24094

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming, human pathogen

description: Aeromonas sanarellii A2-67 is a facultative anaerobe, mesophilic, Gram-negative human pathogen that forms circular colonies and was isolated from abrasion wound of a 70-year-old female.

NCBI tax id

  • NCBI tax id: 633415
  • Matching level: species

strain history

@refhistory
17486<- M. J. Figueras, Rovira i Virgili University, Reus, Spain; A2-67
122410CIP <- 2010, CECT <- M. J. Figueras, Rovira i Virgili Univ., Reus, Spain: strain A2-67

doi: 10.13145/bacdive286.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas sanarellii
  • full scientific name: Aeromonas sanarellii Alperi et al. 2010

@ref: 17486

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas sanarellii

full scientific name: Aeromonas sanarellii Alperi et al. 2010

strain designation: A2-67

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23262negative2.5 µm0.7 µmrod-shapedyes
69480yes98.249
69480negative99.998
122410negativerod-shapedyes

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium usedtype of hemolysishemolysis ability
232624-5 mmbeige, opaquecircular2 daysTSA
232624-5 mmtranslucentcircular2 daysTSA
23262beta1

multimedia

  • @ref: 17486
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_24094.jpg
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17486TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23262MacConkey agaryes
23262Trypticase Soy Agar (TSA)yes
40371MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122410CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
17486positivegrowth28mesophilic
23262positivegrowth45thermophilic
23262nogrowth50thermophilic
23262nogrowth4psychrophilic
23262positiveoptimum30mesophilic
40371positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23262positivegrowth9.0alkaliphile
23262nogrowth4.5

Physiology and metabolism

oxygen tolerance

  • @ref: 122410
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
23262no
69481no98
69480no99.998
122410no

halophily

  • @ref: 23262
  • salt: NaCl
  • growth: no
  • tested relation: maximum
  • concentration: 6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2326217057cellobiose-builds acid from
2326215963ribitol-builds acid from
2326217108D-arabinose-builds acid from
2326218333D-arabitol-builds acid from
2326228847D-fucose-builds acid from
2326262318D-lyxose-builds acid from
2326216024D-mannose-builds acid from
2326217924D-sorbitol-builds acid from
2326216443D-tagatose-builds acid from
2326265327D-xylose-builds acid from
2326216813galactitol-builds acid from
2326217113erythritol-builds acid from
2326228066gentiobiose-builds acid from
2326215443inulin-builds acid from
2326218403L-arabitol-builds acid from
2326218287L-fucose-builds acid from
2326262345L-rhamnose-builds acid from
2326217266L-sorbose-builds acid from
2326265328L-xylose-builds acid from
2326217716lactose-builds acid from
232626731melezitose-builds acid from
2326228053melibiose-builds acid from
23262320061methyl alpha-D-glucopyranoside-builds acid from
2326243943methyl alpha-D-mannoside-builds acid from
2326274863methyl beta-D-xylopyranoside-builds acid from
2326217268myo-inositol-builds acid from
2326216634raffinose-builds acid from
2326232528turanose-builds acid from
2326217151xylitol-builds acid from
2326217234glucose-builds gas from
23262168082-dehydro-D-gluconate-carbon source
23262174265-dehydro-D-gluconate-carbon source
2326217128adipate-carbon source
2326216947citrate-carbon source
2326216024D-mannose-carbon source
2326218401phenylacetate-carbon source
23262168082-dehydro-D-gluconate-energy source
23262174265-dehydro-D-gluconate-energy source
2326217128adipate-energy source
2326216947citrate-energy source
2326216024D-mannose-energy source
2326218401phenylacetate-energy source
232624767elastin-hydrolysis
2326216199urea-hydrolysis
2326227613amygdalin+builds acid from
2326215824D-fructose+builds acid from
2326212936D-galactose+builds acid from
2326217634D-glucose+builds acid from
2326216899D-mannitol+builds acid from
2326216988D-ribose+builds acid from
2326217754glycerol+builds acid from
2326228087glycogen+builds acid from
2326230849L-arabinose+builds acid from
2326217306maltose+builds acid from
23262506227N-acetylglucosamine+builds acid from
2326217814salicin+builds acid from
2326217992sucrose+builds acid from
2326227082trehalose+builds acid from
2326227689decanoate+carbon source
2326217634D-glucose+carbon source
2326216899D-mannitol+carbon source
2326224265gluconate+carbon source
2326230849L-arabinose+carbon source
2326225115malate+carbon source
2326217306maltose+carbon source
23262506227N-acetylglucosamine+carbon source
2326227689decanoate+energy source
2326217634D-glucose+energy source
2326216899D-mannitol+energy source
2326224265gluconate+energy source
2326230849L-arabinose+energy source
2326225115malate+energy source
2326217306maltose+energy source
23262506227N-acetylglucosamine+energy source
2326227897tryptophan+energy source
2326218305arbutin+hydrolysis
2326216991dna+hydrolysis
232624853esculin+hydrolysis
232625291gelatin+hydrolysis
2326216828L-tryptophan+hydrolysis
2326228017starch+hydrolysis
2326217632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
12241017632nitrate+reduction
12241016301nitrite+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantgroup IDis sensitive
232622676amoxicillinyesyes
232622676amoxicillinyesyes11
23262209807cefoxitinyesyes
232623542cephalothinyesyes
2326248923erythromycinyesyes
232629587ticarcillinyesyes
232629587ticarcillinyesyes18
2326273908vibriostatyesyes
232622637amikacinyesyes
23262161680aztreonamyesyes
23262478164cefepimeyesyes
232623493cefoperazoneyesyes
232623508ceftazidimeyesyes
2326229007ceftriaxoneyesyes
23262204928cefotaximeyesyes
2326217698chloramphenicolyesyes
23262100241ciprofloxacinyesyes
2326228915fosfomycinyesyes
2326217833gentamicinyesyes
23262471744imipenemyesyes
232626104kanamycinyesyes
23262100147nalidixic acidyesyes
232628232piperacillinyesyes
232628232piperacillinyes16yes
2326217076streptomycinyesyes
2326227902tetracyclineyesyes
2326228864tobramycinyesyes
2326245924trimethoprimyesyes
2326248947clavulanic acidyesyes11
232629421tazobactamyes16yes
2326248947clavulanic acidyesyes18

metabolite production

@refChebi-IDmetaboliteproduction
2326216136hydrogen sulfideno
2326215688acetoinno
2326235581indoleyes
12241035581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testcitrate test
2326215688acetoin-
2326235581indole+
2326216947citrate-

enzymes

@refvalueactivityec
23262arginine dihydrolase+3.5.3.6
23262beta-galactosidase+3.2.1.23
23262catalase+1.11.1.6
23262cytochrome oxidase+1.9.3.1
23262lysine decarboxylase-4.1.1.18
23262ornithine decarboxylase-4.1.1.17
23262tryptophan deaminase+4.1.99.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122410oxidase+
122410catalase+1.11.1.6
122410urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122410-++++++++-++-+++++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122410+--++----+++-----+---+-+++-+--++---++---------+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
17486abrasion wound of a 70-year-old femaleSpainESPEurope
122410Human, Wound cultureTaiwanTWNAsiaTainan2000

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host#Human#Female
#Host Body-Site#Other#Wound

taxonmaps

  • @ref: 69479
  • File name: preview.99_19.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_19&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: FJ230076
  • Sequence Identity:
  • Total samples: 1087
  • soil counts: 45
  • aquatic counts: 400
  • animal counts: 595
  • plant counts: 47

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
17486yes, in single cases1Risk group (German classification)
1224101Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17486
  • description: Aeromonas sanarellii strain A2-67 16S ribosomal RNA gene, partial sequence
  • accession: FJ230076
  • length: 1503
  • database: ena
  • NCBI tax ID: 633415

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas sanarellii LMG 24682GCA_000820085scaffoldncbi633415
66792Aeromonas sanarellii LMG 24682633415.3wgspatric633415
66792Aeromonas sanarellii LMG 246822639763092draftimg633415

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
motileyes90.925yes
flagellatedyes90.602no
gram-positiveno98.963yes
anaerobicno97.777no
aerobicyes83.969no
halophileno91.522no
spore-formingno96.448no
glucose-utilyes91.022yes
thermophileno99.691no
glucose-fermentyes86.853no

External links

@ref: 17486

culture collection no.: DSM 24094, CECT 7402, LMG 24682, CIP 110203

straininfo link

  • @ref: 69968
  • straininfo: 359330

literature

  • topic: Phylogeny
  • Pubmed-ID: 19819994
  • title: Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan.
  • authors: Alperi A, Martinez-Murcia AJ, Ko WC, Monera A, Saavedra MJ, Figueras MJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.014621-0
  • year: 2009
  • mesh: Aeromonas/*classification/genetics/*isolation & purification, Aged, Bacterial Proteins/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Gram-Negative Bacterial Infections/*microbiology, Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Taiwan
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17486Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24094)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24094
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23262Anabel Alperi, Antonio J. Martínez-Murcia, Wen-Chien Ko, Arturo Monera, Maria J. Saavedra, Maria J. Figueras10.1099/ijs.0.014621-0Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from TaiwanIJSEM 60: 2048-2055 201019819994
40371Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8037
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69968Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID359330.1StrainInfo: A central database for resolving microbial strain identifiers
122410Curators of the CIPCollection of Institut Pasteur (CIP 110203)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110203