Strain identifier

BacDive ID: 2808

Type strain: Yes

Species: Hungatella hathewayi

Strain Designation: 1313

Strain history: CIP <- 2007, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10224 <- DSMZ <- H. Hippe <- P.A. Lawson <- T. Steer: strain 1313

NCBI tax ID(s): 566550 (strain), 154046 (species)

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General

@ref: 5056

BacDive-ID: 2808

DSM-Number: 13479

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive

description: Hungatella hathewayi 1313 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from human faeces.

NCBI tax id

NCBI tax idMatching level
154046species
566550strain

strain history

@refhistory
5056<- H. Hippe, Leibniz-Institut DSMZ GmbH, Braunschweig, Germany; 1313 <- P. A. Lawson <- T. Steer {1999}
119849CIP <- 2007, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10224 <- DSMZ <- H. Hippe <- P.A. Lawson <- T. Steer: strain 1313

doi: 10.13145/bacdive2808.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Hungatella
  • species: Hungatella hathewayi
  • full scientific name: Hungatella hathewayi (Steer et al. 2002) Kaur et al. 2014

  • synonyms

    • @ref: 20215
    • synonym: Clostridium hathewayi

@ref: 5056

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Hungatella

species: Hungatella hathewayi

full scientific name: Hungatella hathewayi (Steer et al. 2002) Kaur et al. 2014

strain designation: 1313

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive99.738
119849positiverod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5056PY + X MEDIUM (DSMZ Medium 104b)yeshttps://mediadive.dsmz.de/medium/104bName: PY + X MEDIUM (DSMZ Medium 104b; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water
37557MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
119849CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
5056positivegrowth30mesophilic
37557positivegrowth37mesophilic
55902positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5056anaerobe
55902anaerobe
69480anaerobe99.587
119849anaerobe

spore formation

  • @ref: 69481
  • spore formation: yes
  • confidence: 90

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11984917632nitrate+reduction
11984916301nitrite-reduction

metabolite production

  • @ref: 119849
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
119849oxidase-
119849catalase-1.11.1.6
119849urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119849----------+-+-+++---

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
5056human faecesUnited KingdomGBREurope
55902Human feces,male,omnivore chemostat enrichmentUnited KingdomGBREurope1999Reading
119849Human, Faeces

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50561Risk group (German classification)
1198492Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium hathewayi strain DSM 13479 16S ribosomal RNA gene, partial sequenceHM245944846ena154046
5056Clostridium hathewayi 16S rRNA geneAJ3116201493ena154046

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Hungatella hathewayi DSM 13479GCA_000160095scaffoldncbi566550
66792Clostridium hathewayi DSM 13479566550.8wgspatric566550
66792Hungatella hathewayi DSM 13479645951835draftimg566550
66792Hungatella hathewayi DSM 13479GCA_025149285completencbi154046
66792Hungatella hathewayi strain DSM 13479154046.361completepatric154046

GC content

  • @ref: 5056
  • GC-content: 50.7-50.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes90no
motileyes74.11no
flagellatedno56.41no
gram-positiveyes83.608no
anaerobicyes97.89no
aerobicno96.359yes
halophileno85.242no
spore-formingyes81.477no
glucose-fermentyes75.847no
thermophileno98.766yes
glucose-utilyes91.683no

External links

@ref: 5056

culture collection no.: DSM 13479, CCUG 43506, CIP 109440, MTCC 10951, AIP 10224

straininfo link

  • @ref: 72334
  • straininfo: 100282

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11822669Clostridium hathewayi sp. nov., from human faeces.Steer T, Collins MD, Gibson GR, Hippe H, Lawson PASyst Appl Microbiol10.1078/0723-2020-000442001Clostridium/classification/*isolation & purification/metabolism, Feces/*microbiology, Female, Humans, Male, Phylogeny, RNA, Ribosomal/geneticsPathogenicity
Phylogeny24186873Hungatella effluvii gen. nov., sp. nov., an obligately anaerobic bacterium isolated from an effluent treatment plant, and reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov.Kaur S, Yawar M, Kumar PA, Suresh KInt J Syst Evol Microbiol10.1099/ijs.0.056986-02013Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, Probiotics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5056Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13479)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13479
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37557Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7184
55902Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43506)https://www.ccug.se/strain?id=43506
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72334Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100282.1StrainInfo: A central database for resolving microbial strain identifiers
119849Curators of the CIPCollection of Institut Pasteur (CIP 109440)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109440